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- Publisher Website: 10.1093/molehr/7.11.1047
- Scopus: eid_2-s2.0-0034753316
- PMID: 11675471
- WOS: WOS:000172030400006
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Article: Quantification of transforming growth factor β1 (TGFβ1) mRNA expression in mouse preimplantation embryos and determination of TGFβ receptor (type I and type II) expression in mouse embryos and reproductive tract
Title | Quantification of transforming growth factor β1 (TGFβ1) mRNA expression in mouse preimplantation embryos and determination of TGFβ receptor (type I and type II) expression in mouse embryos and reproductive tract |
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Authors | |
Keywords | Embryo Oviduct Receptor TGFβ1 Uterus |
Issue Date | 2001 |
Publisher | Oxford University Press. The Journal's web site is located at http://molehr.oxfordjournals.org/ |
Citation | Molecular Human Reproduction, 2001, v. 7 n. 11, p. 1047-1056 How to Cite? |
Abstract | We hypothesized that transforming growth factor β1 (TGFβ 1) and its receptors play a role in the interaction between the preimplantation embryo and the reproductive tract. To investigate this hypothesis, TGFβ 1 mRNA in mouse embryos was quantified by competitive reverse transcription-polymerase chain reaction using an RNA mimic. TGFβ 1 was first detected in the unfertilized oocyte, disappeared after fertilization and was expressed again at the 2-cell stage (4410 ± 1330 transcripts/embryo). Its expression increased gradually, peaked at the 8-cell stage (58 600 ± 17 300 transcripts/embryo) and declined rapidly after the morula stage reaching a concentration of 1520 ± 546 transcripts/embryo at the blastocyst stage. The mRNA levels of TGFβ 1 at the 8-cell and morula stages were significantly higher than that at other cell stages (P < 0.05). The expression of TGF receptors in embryos and in the reproductive tract was also investigated. Both TGFβ 1 type I (ALK-5) and type II TGFβ receptors were detected in embryos from 1-cell to blastocyst stage by immunohistochemistry. Northern hybridization and immunohistochemistry showed a constant expression of both TGFβ receptors in the oviduct from day 1 to day 4 of pregnancy, whilst in the uterus there was a marked increase in the expression of TGFβ type I receptor on day 3. Expression of TGFβ type II receptor in the uterus remained unaltered throughout the study period. This study has shown that preimplantation mouse embryos produce TGFβ 1 and that both the embryos and the reproductive tract are responsive to TGFβ 1 in the preimplantation period. |
Persistent Identifier | http://hdl.handle.net/10722/173249 |
ISSN | 2023 Impact Factor: 3.6 2023 SCImago Journal Rankings: 1.201 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chow, JFC | en_US |
dc.contributor.author | Lee, KF | en_US |
dc.contributor.author | Chan, STH | en_US |
dc.contributor.author | Yeung, WSB | en_US |
dc.date.accessioned | 2012-10-30T06:28:46Z | - |
dc.date.available | 2012-10-30T06:28:46Z | - |
dc.date.issued | 2001 | en_US |
dc.identifier.citation | Molecular Human Reproduction, 2001, v. 7 n. 11, p. 1047-1056 | en_US |
dc.identifier.issn | 1360-9947 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/173249 | - |
dc.description.abstract | We hypothesized that transforming growth factor β1 (TGFβ 1) and its receptors play a role in the interaction between the preimplantation embryo and the reproductive tract. To investigate this hypothesis, TGFβ 1 mRNA in mouse embryos was quantified by competitive reverse transcription-polymerase chain reaction using an RNA mimic. TGFβ 1 was first detected in the unfertilized oocyte, disappeared after fertilization and was expressed again at the 2-cell stage (4410 ± 1330 transcripts/embryo). Its expression increased gradually, peaked at the 8-cell stage (58 600 ± 17 300 transcripts/embryo) and declined rapidly after the morula stage reaching a concentration of 1520 ± 546 transcripts/embryo at the blastocyst stage. The mRNA levels of TGFβ 1 at the 8-cell and morula stages were significantly higher than that at other cell stages (P < 0.05). The expression of TGF receptors in embryos and in the reproductive tract was also investigated. Both TGFβ 1 type I (ALK-5) and type II TGFβ receptors were detected in embryos from 1-cell to blastocyst stage by immunohistochemistry. Northern hybridization and immunohistochemistry showed a constant expression of both TGFβ receptors in the oviduct from day 1 to day 4 of pregnancy, whilst in the uterus there was a marked increase in the expression of TGFβ type I receptor on day 3. Expression of TGFβ type II receptor in the uterus remained unaltered throughout the study period. This study has shown that preimplantation mouse embryos produce TGFβ 1 and that both the embryos and the reproductive tract are responsive to TGFβ 1 in the preimplantation period. | en_US |
dc.language | eng | en_US |
dc.publisher | Oxford University Press. The Journal's web site is located at http://molehr.oxfordjournals.org/ | en_US |
dc.relation.ispartof | Molecular Human Reproduction | en_US |
dc.rights | Molecular Human Reproduction. Copyright © Oxford University Press. | - |
dc.subject | Embryo | - |
dc.subject | Oviduct | - |
dc.subject | Receptor | - |
dc.subject | TGFβ1 | - |
dc.subject | Uterus | - |
dc.subject.mesh | Activin Receptors, Type I - Genetics - Metabolism | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Blastocyst - Physiology | en_US |
dc.subject.mesh | Blotting, Northern | en_US |
dc.subject.mesh | Fallopian Tubes - Physiology | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Gene Expression Regulation, Developmental | en_US |
dc.subject.mesh | Genitalia, Female - Physiology | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Mice | en_US |
dc.subject.mesh | Mice, Inbred Strains | en_US |
dc.subject.mesh | Protein-Serine-Threonine Kinases | en_US |
dc.subject.mesh | Rna, Messenger - Metabolism | en_US |
dc.subject.mesh | Receptors, Transforming Growth Factor Beta - Genetics - Metabolism | en_US |
dc.subject.mesh | Reverse Transcriptase Polymerase Chain Reaction | en_US |
dc.subject.mesh | Sensitivity And Specificity | en_US |
dc.subject.mesh | Transforming Growth Factor Beta - Genetics | en_US |
dc.subject.mesh | Transforming Growth Factor Beta1 | en_US |
dc.subject.mesh | Uterus - Physiology | en_US |
dc.title | Quantification of transforming growth factor β1 (TGFβ1) mRNA expression in mouse preimplantation embryos and determination of TGFβ receptor (type I and type II) expression in mouse embryos and reproductive tract | en_US |
dc.type | Article | en_US |
dc.identifier.email | Lee, KF:ckflee@hku.hk | en_US |
dc.identifier.email | Yeung, WSB:wsbyeung@hkucc.hku.hk | en_US |
dc.identifier.authority | Lee, KF=rp00458 | en_US |
dc.identifier.authority | Yeung, WSB=rp00331 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1093/molehr/7.11.1047 | - |
dc.identifier.pmid | 11675471 | - |
dc.identifier.scopus | eid_2-s2.0-0034753316 | en_US |
dc.identifier.hkuros | 65368 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0034753316&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 7 | en_US |
dc.identifier.issue | 11 | en_US |
dc.identifier.spage | 1047 | en_US |
dc.identifier.epage | 1056 | en_US |
dc.identifier.isi | WOS:000172030400006 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Chow, JFC=7401728953 | en_US |
dc.identifier.scopusauthorid | Lee, KF=26643097500 | en_US |
dc.identifier.scopusauthorid | Chan, STH=24368283200 | en_US |
dc.identifier.scopusauthorid | Yeung, WSB=7102370745 | en_US |
dc.identifier.issnl | 1360-9947 | - |