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- Publisher Website: 10.1210/en.2008-1239
- Scopus: eid_2-s2.0-66449100451
- PMID: 19164472
- WOS: WOS:000265407500048
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Article: Opposite effects of interleukin-1α and transforming growth factor-β2 induce stage-specific regulation of junctional adhesion molecule-B gene in sertoli cells
Title | Opposite effects of interleukin-1α and transforming growth factor-β2 induce stage-specific regulation of junctional adhesion molecule-B gene in sertoli cells |
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Authors | |
Issue Date | 2009 |
Publisher | The Endocrine Society. The Journal's web site is located at http://endo.endojournals.org |
Citation | Endocrinology, 2009, v. 150 n. 5, p. 2404-2412 How to Cite? |
Abstract | In the mammalian testis, junctional adhesion molecule-B (JAM-B) is found at the blood-testis barrier between Sertoli cells and the apical ectoplasmic specializations between Sertoli and germ cells. The expression of JAM-B is tightly regulated to allow the transit of developing germ cells across the blood-testis barrier and the timely release of mature spermatids at stage VIII. In this study, the basal transcription of JAM-B in the mouse Sertoli cell line, MSC-1 cells, was examined. We found that the constitutive expression of JAM-B is carried out by the binding of specificity proteins (Sps), ETS domain transcription factor Elk-1 (Elk1), neuron-restrictive silencer factor (NRSF), and E2F transcription factor 3 (E2F3) to various cis-acting elements including TG interacting factor (TGIF), Elk-1, NRSF, and proximal Sp1 (pSp1) + E2F binding motifs. We also investigated the effects of two cytokines IL-1α and TGF-β2 on JAM-B expression. IL-1α promotes JAM-B expression by facilitating the binding of Elk-1 to TGIF and pSp1 + E2F motifs in a p38-dependent manner, which leads to an additive effect on Sp1- and NRSF-mediated JAM-B transactivation. TGF-/32 inhibits JAM-B transcription via the activation of mothers against decapentaplegic (Smad) proteins and activated Smads compete with specificity proteins (Sp1 and Sp3) for the TGIF motif, resulting in JAM-B repression. IL-1a and Smad3 expression have been reported to be stage specific. IL-1α is absent in theseminferous epithelium at stages VII-VIII, whereas a high level of nuclear Smad3 level is found at the same stages. This study shows for the first time that IL-1 a and TGF-/32 regulate JAM-B expression in an opposite manner, and in vitro data obtained herein provide some clues on how junctions are regulated in the testis. Copyright © 2009 by The Endocrine Society. |
Persistent Identifier | http://hdl.handle.net/10722/179133 |
ISSN | 2023 Impact Factor: 3.8 2023 SCImago Journal Rankings: 1.285 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wang, Y | en_US |
dc.contributor.author | Lui, WY | en_US |
dc.date.accessioned | 2012-12-19T09:52:15Z | - |
dc.date.available | 2012-12-19T09:52:15Z | - |
dc.date.issued | 2009 | en_US |
dc.identifier.citation | Endocrinology, 2009, v. 150 n. 5, p. 2404-2412 | en_US |
dc.identifier.issn | 0013-7227 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/179133 | - |
dc.description.abstract | In the mammalian testis, junctional adhesion molecule-B (JAM-B) is found at the blood-testis barrier between Sertoli cells and the apical ectoplasmic specializations between Sertoli and germ cells. The expression of JAM-B is tightly regulated to allow the transit of developing germ cells across the blood-testis barrier and the timely release of mature spermatids at stage VIII. In this study, the basal transcription of JAM-B in the mouse Sertoli cell line, MSC-1 cells, was examined. We found that the constitutive expression of JAM-B is carried out by the binding of specificity proteins (Sps), ETS domain transcription factor Elk-1 (Elk1), neuron-restrictive silencer factor (NRSF), and E2F transcription factor 3 (E2F3) to various cis-acting elements including TG interacting factor (TGIF), Elk-1, NRSF, and proximal Sp1 (pSp1) + E2F binding motifs. We also investigated the effects of two cytokines IL-1α and TGF-β2 on JAM-B expression. IL-1α promotes JAM-B expression by facilitating the binding of Elk-1 to TGIF and pSp1 + E2F motifs in a p38-dependent manner, which leads to an additive effect on Sp1- and NRSF-mediated JAM-B transactivation. TGF-/32 inhibits JAM-B transcription via the activation of mothers against decapentaplegic (Smad) proteins and activated Smads compete with specificity proteins (Sp1 and Sp3) for the TGIF motif, resulting in JAM-B repression. IL-1a and Smad3 expression have been reported to be stage specific. IL-1α is absent in theseminferous epithelium at stages VII-VIII, whereas a high level of nuclear Smad3 level is found at the same stages. This study shows for the first time that IL-1 a and TGF-/32 regulate JAM-B expression in an opposite manner, and in vitro data obtained herein provide some clues on how junctions are regulated in the testis. Copyright © 2009 by The Endocrine Society. | en_US |
dc.language | eng | en_US |
dc.publisher | The Endocrine Society. The Journal's web site is located at http://endo.endojournals.org | en_US |
dc.relation.ispartof | Endocrinology | en_US |
dc.title | Opposite effects of interleukin-1α and transforming growth factor-β2 induce stage-specific regulation of junctional adhesion molecule-B gene in sertoli cells | en_US |
dc.type | Article | en_US |
dc.identifier.email | Lui, WY: wylui@hku.hk | en_US |
dc.identifier.authority | Lui, WY=rp00756 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1210/en.2008-1239 | en_US |
dc.identifier.pmid | 19164472 | - |
dc.identifier.scopus | eid_2-s2.0-66449100451 | en_US |
dc.identifier.hkuros | 156256 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-66449100451&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 150 | en_US |
dc.identifier.issue | 5 | en_US |
dc.identifier.spage | 2404 | en_US |
dc.identifier.epage | 2412 | en_US |
dc.identifier.eissn | 1945-7170 | - |
dc.identifier.isi | WOS:000265407500048 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Wang, Y=7601513691 | en_US |
dc.identifier.scopusauthorid | Lui, WY=35220192400 | en_US |
dc.identifier.issnl | 0013-7227 | - |