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- Publisher Website: 10.1111/j.1750-3841.2012.02802.x
- Scopus: eid_2-s2.0-84864608780
- PMID: 22809296
- WOS: WOS:000307385800030
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Article: The Impact of NaCl Substitution with KCl on Proteinase Activities Cell-Free Extract and Cell-Free Supernatant at Different pH Levels and Salt Concentrations: Lactobacillus delbrueckii ssp. bulgaricus and Streptococcus thermophilus
Title | The Impact of NaCl Substitution with KCl on Proteinase Activities Cell-Free Extract and Cell-Free Supernatant at Different pH Levels and Salt Concentrations: Lactobacillus delbrueckii ssp. bulgaricus and Streptococcus thermophilus |
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Authors | |
Keywords | Kcl L. Delbrueckii Ssp. Bulgaricus Nacl S. Thermophilus Salt Substitution |
Issue Date | 2012 |
Publisher | Wiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-1147 |
Citation | Journal Of Food Science, 2012, v. 77 n. 8, p. M490-M498 How to Cite? |
Abstract | The effect of NaCl substitution with KCl at different pH levels (6.0, 5.5, and 5.0) and salt concentrations on proteinase activities of cell-free and supernatant of Lactobacillus delbrueckii ssp. bulgaricus 11824 (L. bulgaricus) and Streptococcus thermophilus MS (ST) was investigated. MRS broths were separately mixed with 4 salt treatments (NaCl only, 1NaCl:1KCl, 1NaCl:3KCl, and KCl only) at 2 different concentrations (5% and 10%) and incubated at 37 °C for 22 h. The cell pellets were used to prepare proteinase of cell-free extract and the cell-free supernatants were used as source of extracellular proteinases. The proteolytic activities and protein contents of both fractions were determined. The supernatants after incubation of both fractions with 3 milk caseins (α-, β-, κ-casein) were subjected to angiotensin-converting-enzyme inhibitory (ACE-inhibitory) activity and proteolytic activity by ortho-phthalaldehyde (OPA) method. Significant differences were observed in ACE-inhibitory activities and proteolytic (OPA) between salt treatments of cell-free extract and cell-free supernatant of L. bulgaricus and S. thermophilus at same salt concentration and same pH level. There was a significant effect of pH level and salt treatments interaction on ACE-inhibitory activity, OPA activity and azocasein activity. Practical Application: To reduce sodium concentration in cheese by substituting of NaCl with KCl, it was important to study the effect on starter culture proteinases which play a vital role in ripening and texture profile of cheese. © 2012 Institute of Food Technologists ®. |
Persistent Identifier | http://hdl.handle.net/10722/179295 |
ISSN | 2023 Impact Factor: 3.2 2023 SCImago Journal Rankings: 0.783 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ayyash, MM | en_US |
dc.contributor.author | Sherkat, F | en_US |
dc.contributor.author | Shah, NP | en_US |
dc.date.accessioned | 2012-12-19T09:53:55Z | - |
dc.date.available | 2012-12-19T09:53:55Z | - |
dc.date.issued | 2012 | en_US |
dc.identifier.citation | Journal Of Food Science, 2012, v. 77 n. 8, p. M490-M498 | en_US |
dc.identifier.issn | 0022-1147 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/179295 | - |
dc.description.abstract | The effect of NaCl substitution with KCl at different pH levels (6.0, 5.5, and 5.0) and salt concentrations on proteinase activities of cell-free and supernatant of Lactobacillus delbrueckii ssp. bulgaricus 11824 (L. bulgaricus) and Streptococcus thermophilus MS (ST) was investigated. MRS broths were separately mixed with 4 salt treatments (NaCl only, 1NaCl:1KCl, 1NaCl:3KCl, and KCl only) at 2 different concentrations (5% and 10%) and incubated at 37 °C for 22 h. The cell pellets were used to prepare proteinase of cell-free extract and the cell-free supernatants were used as source of extracellular proteinases. The proteolytic activities and protein contents of both fractions were determined. The supernatants after incubation of both fractions with 3 milk caseins (α-, β-, κ-casein) were subjected to angiotensin-converting-enzyme inhibitory (ACE-inhibitory) activity and proteolytic activity by ortho-phthalaldehyde (OPA) method. Significant differences were observed in ACE-inhibitory activities and proteolytic (OPA) between salt treatments of cell-free extract and cell-free supernatant of L. bulgaricus and S. thermophilus at same salt concentration and same pH level. There was a significant effect of pH level and salt treatments interaction on ACE-inhibitory activity, OPA activity and azocasein activity. Practical Application: To reduce sodium concentration in cheese by substituting of NaCl with KCl, it was important to study the effect on starter culture proteinases which play a vital role in ripening and texture profile of cheese. © 2012 Institute of Food Technologists ®. | en_US |
dc.language | eng | en_US |
dc.publisher | Wiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-1147 | en_US |
dc.relation.ispartof | Journal of Food Science | en_US |
dc.subject | Kcl | en_US |
dc.subject | L. Delbrueckii Ssp. Bulgaricus | en_US |
dc.subject | Nacl | en_US |
dc.subject | S. Thermophilus | en_US |
dc.subject | Salt Substitution | en_US |
dc.title | The Impact of NaCl Substitution with KCl on Proteinase Activities Cell-Free Extract and Cell-Free Supernatant at Different pH Levels and Salt Concentrations: Lactobacillus delbrueckii ssp. bulgaricus and Streptococcus thermophilus | en_US |
dc.type | Article | en_US |
dc.identifier.email | Shah, NP: npshah@hku.hk | en_US |
dc.identifier.authority | Shah, NP=rp01571 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1111/j.1750-3841.2012.02802.x | en_US |
dc.identifier.pmid | 22809296 | - |
dc.identifier.scopus | eid_2-s2.0-84864608780 | en_US |
dc.identifier.hkuros | 205697 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-84864608780&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 77 | en_US |
dc.identifier.issue | 8 | en_US |
dc.identifier.spage | M490 | en_US |
dc.identifier.epage | M498 | en_US |
dc.identifier.eissn | 1750-3841 | - |
dc.identifier.isi | WOS:000307385800030 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Ayyash, MM=16202006000 | en_US |
dc.identifier.scopusauthorid | Sherkat, F=6602248294 | en_US |
dc.identifier.scopusauthorid | Shah, NP=7401823907 | en_US |
dc.identifier.issnl | 0022-1147 | - |