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- Publisher Website: 10.3168/jds.2012-5761
- Scopus: eid_2-s2.0-84871621229
- PMID: 23084889
- WOS: WOS:000312524300006
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Article: The effect of NaCl substitution with KCl on proteinase activities of cell-free extract and cell-free supernatant at different pH levels and salt concentrations: Lactobacillus acidophilus and Lactobacillus casei
Title | The effect of NaCl substitution with KCl on proteinase activities of cell-free extract and cell-free supernatant at different pH levels and salt concentrations: Lactobacillus acidophilus and Lactobacillus casei |
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Authors | |
Keywords | Kcl Lactobacillus Acidophilus Lactobacillus Casei Salt Substitution |
Issue Date | 2013 |
Publisher | Elsevier Inc. The Journal's web site is located at http://www.journalofdairyscience.org/ |
Citation | Journal Of Dairy Science, 2013, v. 96 n. 1, p. 40-49 How to Cite? |
Abstract | The aim of this study was to investigate the effect of substitution of NaCl with KCl at different pH levels and salt concentrations on proteinase activity of cell-free extract and cell-free supernatant of the probiotics Lactobacillus acidophilus and Lactobacillus casei. de Man, Rogosa, and Sharpe broth aliquots were mixed with 2 pure salts (NaCl and KCl) and 2 salt concentrations at 2 concentration levels (5 and 10%), inoculated with Lactobacillus acidophilus or Lactobacillus casei, and incubated aerobically at 37°C for 22 h. The cultures were then centrifuged at 4,000 ×g for 30 min, and the collected cell pellets were used to prepare cell-wall proteinases and the supernatants used as a source of supernatant (extracellular) proteinases. The proteolytic activity and protein content of both portions were determined. After incubation of both portions with 3 milk caseins (α-, β-, κ-casein), the supernatants were individually subjected to analysis of angiotensin-converting enzyme (ACE)-inhibitory activity and proteolytic activity using the o-phthalaldehyde method. Significant differences were observed in ACE-inhibitory and proteolytic activities between salt substitution treatments of cell-free extract and cell-free supernatant from both probiotic strains at the same salt concentration and pH level. © 2013 American Dairy Science Association. |
Persistent Identifier | http://hdl.handle.net/10722/179322 |
ISSN | 2023 Impact Factor: 3.7 2023 SCImago Journal Rankings: 1.219 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Ayyash, MM | en_US |
dc.contributor.author | Sherkat, F | en_US |
dc.contributor.author | Shah, NP | en_US |
dc.date.accessioned | 2012-12-19T09:54:09Z | - |
dc.date.available | 2012-12-19T09:54:09Z | - |
dc.date.issued | 2013 | en_US |
dc.identifier.citation | Journal Of Dairy Science, 2013, v. 96 n. 1, p. 40-49 | en_US |
dc.identifier.issn | 0022-0302 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/179322 | - |
dc.description.abstract | The aim of this study was to investigate the effect of substitution of NaCl with KCl at different pH levels and salt concentrations on proteinase activity of cell-free extract and cell-free supernatant of the probiotics Lactobacillus acidophilus and Lactobacillus casei. de Man, Rogosa, and Sharpe broth aliquots were mixed with 2 pure salts (NaCl and KCl) and 2 salt concentrations at 2 concentration levels (5 and 10%), inoculated with Lactobacillus acidophilus or Lactobacillus casei, and incubated aerobically at 37°C for 22 h. The cultures were then centrifuged at 4,000 ×g for 30 min, and the collected cell pellets were used to prepare cell-wall proteinases and the supernatants used as a source of supernatant (extracellular) proteinases. The proteolytic activity and protein content of both portions were determined. After incubation of both portions with 3 milk caseins (α-, β-, κ-casein), the supernatants were individually subjected to analysis of angiotensin-converting enzyme (ACE)-inhibitory activity and proteolytic activity using the o-phthalaldehyde method. Significant differences were observed in ACE-inhibitory and proteolytic activities between salt substitution treatments of cell-free extract and cell-free supernatant from both probiotic strains at the same salt concentration and pH level. © 2013 American Dairy Science Association. | en_US |
dc.language | eng | en_US |
dc.publisher | Elsevier Inc. The Journal's web site is located at http://www.journalofdairyscience.org/ | en_US |
dc.relation.ispartof | Journal of Dairy Science | en_US |
dc.subject | Kcl | en_US |
dc.subject | Lactobacillus Acidophilus | en_US |
dc.subject | Lactobacillus Casei | en_US |
dc.subject | Salt Substitution | en_US |
dc.title | The effect of NaCl substitution with KCl on proteinase activities of cell-free extract and cell-free supernatant at different pH levels and salt concentrations: Lactobacillus acidophilus and Lactobacillus casei | en_US |
dc.type | Article | en_US |
dc.identifier.email | Shah, NP: npshah@hku.hk | en_US |
dc.identifier.authority | Shah, NP=rp01571 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.3168/jds.2012-5761 | en_US |
dc.identifier.pmid | 23084889 | - |
dc.identifier.scopus | eid_2-s2.0-84871621229 | en_US |
dc.identifier.hkuros | 240690 | - |
dc.identifier.isi | WOS:000312524300006 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Ayyash, MM=16202006000 | en_US |
dc.identifier.scopusauthorid | Sherkat, F=6602248294 | en_US |
dc.identifier.scopusauthorid | Shah, NP=7401823907 | en_US |
dc.identifier.issnl | 0022-0302 | - |