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Conference Paper: Preventing root caries development under oral biofilm challenge
Title | Preventing root caries development under oral biofilm challenge |
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Authors | |
Keywords | Antimicrobials Biofilm Caries Collagen and Demineralization |
Issue Date | 2013 |
Publisher | Sage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925 |
Citation | The 91st General Session & Exhibition of the International Association for Dental Research (IADR), Seattle, Washington, USA, 20-23 March 2013. In Journal of Dental Research, 2013, v. 92 n. Special Issue A: abstract no. 3277 How to Cite? |
Abstract | Objectives: To study the preventive effects of chlorhexidine against root caries under oral biofilm in an artificial mouth.
Methods: Sixteen human tooth-root disks were inoculated with a salivary sample that was produced by mixing the unstimulated saliva of three adults who had no untreated caries. The disks were incubated in an artificial mouth fed with a 5% sucrose solution three times daily for one week. Eight disks received a twice daily rinse of 0.12% chlorhexidine (test group). The other eight disks were rinsed in distilled water (control). The biofilm was then studied with three techniques: colony forming unit (CFU) counting, scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). The changes in the chemical structure of the root surface were studied using Fourier transform infra-Red spectroscopy. Type-I collagen and proteoglycans on the root surface were quantified using immunocytochemical staining.
Results: The log CFU for the test and control groups were 4.21 and 8.27, respectively (p<0.001). The CFU count of Streptococci and Lactobacilli were negligible. Both the SEM and the CLSM showed suppressed bacteria growth in the test group. The log [amide-I: HPO42-] of the test and control groups were 1.11 and 1.93, respectively (p=0.02). The mean counts of sound type-I collagen in the test and control groups were 16.8/μm2 and 13.0/μm2, respectively (p<0.001), whereas the mean counts of intact proteoglycans were 5.6/μm2 and 3.5/μm2, respectively (P<0.001).
Conclusions: Chlorhexidine suppressed the growth of selected cariogenic bacteria in oral biofilm on the root surface and thus protected tooth-root from cariogenic challenge.
This abstract is based on research that was funded entirely or partially by an outside source: This study was supported by the Research Grant Council (Hong Kong) General Research Fund (Grant No. HKU 765111M). |
Description | Poster Presentation Session 384: Cariogenic Biofilms Composition and Properties |
Persistent Identifier | http://hdl.handle.net/10722/183218 |
ISSN | 2023 Impact Factor: 5.7 2023 SCImago Journal Rankings: 1.909 |
DC Field | Value | Language |
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dc.contributor.author | Mei, L | en_US |
dc.contributor.author | Chu, CH | en_US |
dc.contributor.author | Lo, ECM | en_US |
dc.contributor.author | Samaranayake, LP | en_US |
dc.date.accessioned | 2013-05-15T01:48:18Z | - |
dc.date.available | 2013-05-15T01:48:18Z | - |
dc.date.issued | 2013 | en_US |
dc.identifier.citation | The 91st General Session & Exhibition of the International Association for Dental Research (IADR), Seattle, Washington, USA, 20-23 March 2013. In Journal of Dental Research, 2013, v. 92 n. Special Issue A: abstract no. 3277 | en_US |
dc.identifier.issn | 0022-0345 | - |
dc.identifier.uri | http://hdl.handle.net/10722/183218 | - |
dc.description | Poster Presentation | - |
dc.description | Session 384: Cariogenic Biofilms Composition and Properties | - |
dc.description.abstract | Objectives: To study the preventive effects of chlorhexidine against root caries under oral biofilm in an artificial mouth. Methods: Sixteen human tooth-root disks were inoculated with a salivary sample that was produced by mixing the unstimulated saliva of three adults who had no untreated caries. The disks were incubated in an artificial mouth fed with a 5% sucrose solution three times daily for one week. Eight disks received a twice daily rinse of 0.12% chlorhexidine (test group). The other eight disks were rinsed in distilled water (control). The biofilm was then studied with three techniques: colony forming unit (CFU) counting, scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). The changes in the chemical structure of the root surface were studied using Fourier transform infra-Red spectroscopy. Type-I collagen and proteoglycans on the root surface were quantified using immunocytochemical staining. Results: The log CFU for the test and control groups were 4.21 and 8.27, respectively (p<0.001). The CFU count of Streptococci and Lactobacilli were negligible. Both the SEM and the CLSM showed suppressed bacteria growth in the test group. The log [amide-I: HPO42-] of the test and control groups were 1.11 and 1.93, respectively (p=0.02). The mean counts of sound type-I collagen in the test and control groups were 16.8/μm2 and 13.0/μm2, respectively (p<0.001), whereas the mean counts of intact proteoglycans were 5.6/μm2 and 3.5/μm2, respectively (P<0.001). Conclusions: Chlorhexidine suppressed the growth of selected cariogenic bacteria in oral biofilm on the root surface and thus protected tooth-root from cariogenic challenge. This abstract is based on research that was funded entirely or partially by an outside source: This study was supported by the Research Grant Council (Hong Kong) General Research Fund (Grant No. HKU 765111M). | - |
dc.language | eng | en_US |
dc.publisher | Sage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925 | - |
dc.relation.ispartof | Journal of Dental Research | en_US |
dc.rights | Journal of Dental Research. Copyright © Sage Publications, Inc. | - |
dc.subject | Antimicrobials | - |
dc.subject | Biofilm | - |
dc.subject | Caries | - |
dc.subject | Collagen and Demineralization | - |
dc.title | Preventing root caries development under oral biofilm challenge | en_US |
dc.type | Conference_Paper | en_US |
dc.identifier.email | Mei, L: leimei@hkusua.hku.hk | en_US |
dc.identifier.email | Chu, CH: chchu@hku.hk | en_US |
dc.identifier.email | Lo, ECM: hrdplcm@hkucc.hku.hk | en_US |
dc.identifier.email | Samaranayake, LP: lakshman@hku.hk | en_US |
dc.identifier.authority | Mei, L=rp01840 | en_US |
dc.identifier.authority | Chu, CH=rp00022 | en_US |
dc.identifier.authority | Lo, ECM=rp00015 | en_US |
dc.identifier.hkuros | 214397 | en_US |
dc.identifier.volume | 92 | en_US |
dc.identifier.issue | Special Issue A: abstract no. 3277 | en_US |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0022-0345 | - |