File Download
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1063/1.4817769
- Scopus: eid_2-s2.0-84883311013
- PMID: 24404050
- WOS: WOS:000323907600019
- Find via
Supplementary
- Citations:
- Appears in Collections:
Conference Paper: Fabrication of uniform multi-compartment particles using microfludic electrospray technology for cell co-culture studies
Title | Fabrication of uniform multi-compartment particles using microfludic electrospray technology for cell co-culture studies |
---|---|
Authors | |
Keywords | Cell-cell interaction Extracellular matrices High field strengths Hydrogel particles Mechanical environment Monodisperse droplets Multiple compartments Significant mixing |
Issue Date | 2013 |
Publisher | American Institute of Physics. The Journal's web site is located at http://bmf.aip.org |
Citation | The 3rd European Conference on Microfluidics (μFlu'12), Heidelberg, Germany, 3-5 December 2012. In Biomicrofluidics, 2013, v. 7 n. 4, article no. 044117, p. 1-8 How to Cite? |
Abstract | In this work, we demonstrate a robust and reliable approach to fabricate multi-compartment particles for cell co-culture studies. By taking advantage of the laminar flow within our microfluidic nozzle, multiple parallel streams of liquids flow towards the nozzle without significant mixing. Afterwards, the multiple parallel streams merge into a single stream, which is sprayed into air, forming monodisperse droplets under an electric field with a high field strength. The resultant multi-compartment droplets are subsequently cross-linked in a calcium chloride solution to form calcium alginate micro-particles with multiple compartments. Each compartment of the particles can be used for encapsulating different types of cells or biological cell factors. These hydrogel particles with cross-linked alginate chains show similarity in the physical and mechanical environment as the extracellular matrix of biological cells. Thus, the multi-compartment particles provide a promising platform for cell studies and co-culture of different cells. In our study, cells are encapsulated in the multi-compartment particles and the viability of cells is quantified using a fluorescence microscope after the cells are stained for a live/dead assay. The high cell viability after encapsulation indicates the cytocompatibility and feasibility of our technique. Our multi-compartment particles have great potential as a platform for studying cell-cell interactions as well as interactions of cells with extracellular factors. © 2013 AIP Publishing LLC. |
Description | Footnote in article: Paper submitted as part of the 3rd European Conference on Microfluidics ... 2012 |
Persistent Identifier | http://hdl.handle.net/10722/190009 |
ISSN | 2023 Impact Factor: 2.6 2023 SCImago Journal Rankings: 0.516 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Liu, Z | en_US |
dc.contributor.author | Shum, HC | en_US |
dc.date.accessioned | 2013-09-17T15:04:37Z | - |
dc.date.available | 2013-09-17T15:04:37Z | - |
dc.date.issued | 2013 | en_US |
dc.identifier.citation | The 3rd European Conference on Microfluidics (μFlu'12), Heidelberg, Germany, 3-5 December 2012. In Biomicrofluidics, 2013, v. 7 n. 4, article no. 044117, p. 1-8 | - |
dc.identifier.issn | 1932-1058 | - |
dc.identifier.uri | http://hdl.handle.net/10722/190009 | - |
dc.description | Footnote in article: Paper submitted as part of the 3rd European Conference on Microfluidics ... 2012 | - |
dc.description.abstract | In this work, we demonstrate a robust and reliable approach to fabricate multi-compartment particles for cell co-culture studies. By taking advantage of the laminar flow within our microfluidic nozzle, multiple parallel streams of liquids flow towards the nozzle without significant mixing. Afterwards, the multiple parallel streams merge into a single stream, which is sprayed into air, forming monodisperse droplets under an electric field with a high field strength. The resultant multi-compartment droplets are subsequently cross-linked in a calcium chloride solution to form calcium alginate micro-particles with multiple compartments. Each compartment of the particles can be used for encapsulating different types of cells or biological cell factors. These hydrogel particles with cross-linked alginate chains show similarity in the physical and mechanical environment as the extracellular matrix of biological cells. Thus, the multi-compartment particles provide a promising platform for cell studies and co-culture of different cells. In our study, cells are encapsulated in the multi-compartment particles and the viability of cells is quantified using a fluorescence microscope after the cells are stained for a live/dead assay. The high cell viability after encapsulation indicates the cytocompatibility and feasibility of our technique. Our multi-compartment particles have great potential as a platform for studying cell-cell interactions as well as interactions of cells with extracellular factors. © 2013 AIP Publishing LLC. | - |
dc.language | eng | en_US |
dc.publisher | American Institute of Physics. The Journal's web site is located at http://bmf.aip.org | - |
dc.relation.ispartof | Biomicrofluidics | en_US |
dc.rights | Copyright 2013 American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. The following article appeared in Biomicrofluidics, 2013, v. 7 n. 4, article no. 044117, p. 1-8 and may be found at https://doi.org/10.1063/1.4817769 | - |
dc.subject | Cell-cell interaction | - |
dc.subject | Extracellular matrices | - |
dc.subject | High field strengths | - |
dc.subject | Hydrogel particles | - |
dc.subject | Mechanical environment | - |
dc.subject | Monodisperse droplets | - |
dc.subject | Multiple compartments | - |
dc.subject | Significant mixing | - |
dc.title | Fabrication of uniform multi-compartment particles using microfludic electrospray technology for cell co-culture studies | en_US |
dc.type | Conference_Paper | en_US |
dc.identifier.email | Shum, HC: ashum@hku.hk | en_US |
dc.identifier.authority | Shum, HC=rp01439 | en_US |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1063/1.4817769 | - |
dc.identifier.pmid | 24404050 | - |
dc.identifier.pmcid | PMC3758358 | - |
dc.identifier.scopus | eid_2-s2.0-84883311013 | - |
dc.identifier.hkuros | 223197 | en_US |
dc.identifier.hkuros | 223302 | - |
dc.identifier.volume | 7 | - |
dc.identifier.issue | 4 | - |
dc.identifier.spage | article no. 044117, p. 1 | - |
dc.identifier.epage | article no. 044117, p. 8 | - |
dc.identifier.isi | WOS:000323907600019 | - |
dc.publisher.place | United States | - |
dc.customcontrol.immutable | sml 140102 | - |
dc.identifier.issnl | 1932-1058 | - |