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- Scopus: eid_2-s2.0-0024432530
- PMID: 2679131
- WOS: WOS:A1989CC64500048
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Article: Diabetes decreases creatine kinase enzyme activity and mRNA level in the rat heart
Title | Diabetes decreases creatine kinase enzyme activity and mRNA level in the rat heart |
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Authors | |
Issue Date | 1989 |
Citation | American Journal of Physiology - Endocrinology and Metabolism, 1989, v. 257 n. 4, p. 20/4 How to Cite? |
Abstract | Several of the adenosinetriphosphatase enzymes that are responsible for cardiac muscle contraction rely on high-energy phosphates supplied by the creatine kinase (CK) system. Experimental diabetes mellitus has been shown to cause a decrease in the maximal contractile performance of the heart. We postulated that the decrease in contractile performance may be explained in part by a decrease in CK enzyme activity. To evaluate this possibility, we determined the level of CK activity and isoenzyme distribution in ventricular homogenates from normal, diabetic, and insulin-treated diabetic rats. We found that total CK activity was decreased by 35% in diabetic hearts and that a 66% reduction in the cardiac-specific MB isoenzyme occurs. Using a cDNA probe for CK-muscle (M) RNA in Northern blot analysis, we determined that a 61.1% decrease in CK-M mRNA occurs in diabetes. Chronic insulin therapy for 1 mo restores CK-M mRNA levels and enzyme activity. In conclusion, diabetes-induced CK enzyme decreases are mediated in part by a lower level of CK-M mRNA that codes for the major CK-M subunit protein. Decreased performance of the CK system may contribute to diabetic cardiomyopathy. |
Persistent Identifier | http://hdl.handle.net/10722/195219 |
ISSN | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Popovich, BK | - |
dc.contributor.author | Boheler, KR | - |
dc.contributor.author | Dillmann, WH | - |
dc.date.accessioned | 2014-02-25T01:40:19Z | - |
dc.date.available | 2014-02-25T01:40:19Z | - |
dc.date.issued | 1989 | - |
dc.identifier.citation | American Journal of Physiology - Endocrinology and Metabolism, 1989, v. 257 n. 4, p. 20/4 | - |
dc.identifier.issn | 0002-9513 | - |
dc.identifier.uri | http://hdl.handle.net/10722/195219 | - |
dc.description.abstract | Several of the adenosinetriphosphatase enzymes that are responsible for cardiac muscle contraction rely on high-energy phosphates supplied by the creatine kinase (CK) system. Experimental diabetes mellitus has been shown to cause a decrease in the maximal contractile performance of the heart. We postulated that the decrease in contractile performance may be explained in part by a decrease in CK enzyme activity. To evaluate this possibility, we determined the level of CK activity and isoenzyme distribution in ventricular homogenates from normal, diabetic, and insulin-treated diabetic rats. We found that total CK activity was decreased by 35% in diabetic hearts and that a 66% reduction in the cardiac-specific MB isoenzyme occurs. Using a cDNA probe for CK-muscle (M) RNA in Northern blot analysis, we determined that a 61.1% decrease in CK-M mRNA occurs in diabetes. Chronic insulin therapy for 1 mo restores CK-M mRNA levels and enzyme activity. In conclusion, diabetes-induced CK enzyme decreases are mediated in part by a lower level of CK-M mRNA that codes for the major CK-M subunit protein. Decreased performance of the CK system may contribute to diabetic cardiomyopathy. | - |
dc.language | eng | - |
dc.relation.ispartof | American Journal of Physiology - Endocrinology and Metabolism | - |
dc.title | Diabetes decreases creatine kinase enzyme activity and mRNA level in the rat heart | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.pmid | 2679131 | - |
dc.identifier.scopus | eid_2-s2.0-0024432530 | - |
dc.identifier.volume | 257 | - |
dc.identifier.issue | 4 | - |
dc.identifier.spage | 20/4 | - |
dc.identifier.epage | 20/4 | - |
dc.identifier.isi | WOS:A1989CC64500048 | - |
dc.identifier.issnl | 0002-9513 | - |