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- Publisher Website: 10.1371/journal.pbio.1001249
- Scopus: eid_2-s2.0-84856480470
- PMID: 22291574
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Article: Stochastic Expression of the Interferon-beta Gene
Title | Stochastic Expression of the Interferon-beta Gene |
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Authors | |
Keywords | Animals Cell Line Fibroblasts/*immunology/virology Flow Cytometry Gene Expression Regulation Genetic Variation Host-Pathogen Interactions Interferon-alpha/biosynthesis/genetics Interferon-beta/biosynthesis/*genetics Membrane Proteins/genetics/metabolism Mice Nerve Tissue Proteins/genetics/metabolism Poly I-C/genetics Recombinant Fusion Proteins/genetics/immunology Sendai virus/growth & development/*immunology Signal Transduction Stochastic Processes Transcription Factors/genetics/immunology Transcription, Genetic Virus Replication |
Issue Date | 2012 |
Publisher | Public Library of Science. The Journal's web site is located at http://www.plosbiology.org/plosonline/?request=index-html |
Citation | PLoS Biology, 2012, v. 10 n. 1, p. article no. e1001249 How to Cite? |
Abstract | Virus infection of mammalian cells induces the production of high levels of type I interferons (IFNalpha and beta), cytokines that orchestrate antiviral innate and adaptive immunity. Previous studies have shown that only a fraction of the infected cells produce IFN. However, the mechanisms responsible for this stochastic expression are poorly understood. Here we report an in depth analysis of IFN-expressing and non-expressing mouse cells infected with Sendai virus. Mouse embryonic fibroblasts in which an internal ribosome entry site/yellow fluorescent protein gene was inserted downstream from the endogenous IFNbeta gene were used to distinguish between the two cell types, and they were isolated from each other using fluorescence-activated cell sorting methods. Analysis of the separated cells revealed that stochastic IFNbeta expression is a consequence of cell-to-cell variability in the levels and/or activities of limiting components at every level of the virus induction process, ranging from viral replication and expression, to the sensing of viral RNA by host factors, to activation of the signaling pathway, to the levels of activated transcription factors. We propose that this highly complex stochastic IFNbeta gene expression evolved to optimize both the level and distribution of type I IFNs in response to virus infection. |
Persistent Identifier | http://hdl.handle.net/10722/219914 |
ISSN | 2023 Impact Factor: 7.8 2023 SCImago Journal Rankings: 3.822 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Zhao, M | - |
dc.contributor.author | Zhang, J | - |
dc.contributor.author | Phatnan, H | - |
dc.contributor.author | Scheu, S | - |
dc.contributor.author | Maniatis, T | - |
dc.date.accessioned | 2015-10-02T04:41:05Z | - |
dc.date.available | 2015-10-02T04:41:05Z | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | PLoS Biology, 2012, v. 10 n. 1, p. article no. e1001249 | - |
dc.identifier.issn | 1544-9173 | - |
dc.identifier.uri | http://hdl.handle.net/10722/219914 | - |
dc.description.abstract | Virus infection of mammalian cells induces the production of high levels of type I interferons (IFNalpha and beta), cytokines that orchestrate antiviral innate and adaptive immunity. Previous studies have shown that only a fraction of the infected cells produce IFN. However, the mechanisms responsible for this stochastic expression are poorly understood. Here we report an in depth analysis of IFN-expressing and non-expressing mouse cells infected with Sendai virus. Mouse embryonic fibroblasts in which an internal ribosome entry site/yellow fluorescent protein gene was inserted downstream from the endogenous IFNbeta gene were used to distinguish between the two cell types, and they were isolated from each other using fluorescence-activated cell sorting methods. Analysis of the separated cells revealed that stochastic IFNbeta expression is a consequence of cell-to-cell variability in the levels and/or activities of limiting components at every level of the virus induction process, ranging from viral replication and expression, to the sensing of viral RNA by host factors, to activation of the signaling pathway, to the levels of activated transcription factors. We propose that this highly complex stochastic IFNbeta gene expression evolved to optimize both the level and distribution of type I IFNs in response to virus infection. | - |
dc.language | eng | - |
dc.publisher | Public Library of Science. The Journal's web site is located at http://www.plosbiology.org/plosonline/?request=index-html | - |
dc.relation.ispartof | PLoS Biology | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | Animals | - |
dc.subject | Cell Line | - |
dc.subject | Fibroblasts/*immunology/virology | - |
dc.subject | Flow Cytometry | - |
dc.subject | Gene Expression Regulation | - |
dc.subject | Genetic Variation | - |
dc.subject | Host-Pathogen Interactions | - |
dc.subject | Interferon-alpha/biosynthesis/genetics | - |
dc.subject | Interferon-beta/biosynthesis/*genetics | - |
dc.subject | Membrane Proteins/genetics/metabolism | - |
dc.subject | Mice | - |
dc.subject | Nerve Tissue Proteins/genetics/metabolism | - |
dc.subject | Poly I-C/genetics | - |
dc.subject | Recombinant Fusion Proteins/genetics/immunology | - |
dc.subject | Sendai virus/growth & development/*immunology | - |
dc.subject | Signal Transduction | - |
dc.subject | Stochastic Processes | - |
dc.subject | Transcription Factors/genetics/immunology | - |
dc.subject | Transcription, Genetic | - |
dc.subject | Virus Replication | - |
dc.title | Stochastic Expression of the Interferon-beta Gene | - |
dc.type | Article | - |
dc.identifier.email | Zhang, J: jzhang1@hku.hk | - |
dc.identifier.authority | Zhang, J=rp01713 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1371/journal.pbio.1001249 | - |
dc.identifier.pmid | 22291574 | - |
dc.identifier.pmcid | PMC3265471 | - |
dc.identifier.scopus | eid_2-s2.0-84856480470 | - |
dc.identifier.volume | 10 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | article no. e1001249 | - |
dc.identifier.epage | article no. e1001249 | - |
dc.identifier.isi | WOS:000300420400018 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 1544-9173 | - |