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Conference Paper: Baicalin reduces hemorrhagic transformation of rat ischemic stroke with delayed T-PA treatment
Title | Baicalin reduces hemorrhagic transformation of rat ischemic stroke with delayed T-PA treatment |
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Authors | |
Issue Date | 2016 |
Publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/freeradbiomed |
Citation | SfRBM 2016 & SFRRI 18th Biennial Meeting, San Francisco, CA., 16-19 November 2016. In Free Radical Biology and Medicine, 2016, v. 100 suppl., p. S159, abstract no. 376 How to Cite? |
Abstract | Hemorrhagic transformation (HT) is the severe complication of delayed tissue plasminogen activator (t-PA) treatment in ischemic stroke. Previously we showed that targeting peroxynitrite attenuated HT and improved neurological outcomes in ischemic stroke with thrombolytic therapy. Here we test the hypothesis that baicalin, a natural compound capable of scavenging peroxynitrite, could reduce HT in a rat cerebral ischemia-reperfusion model with delayed t-PA treatment, through targeting peroxynitrite-MMP-9 signaling. Male Sprague-Dawley (SD) rats were subjected to middle cerebral artery occlusion (MCAO) 5 hours plus reperfusion 19 hours. T-PA (10 mg/kg) or t-PA plus baicalin (50, 100, 150 mg/kg) was administrated via femoral vein at 4.5 hours after MCAO. Delayed t-PA treatment increased the mortality rates and further exacerbated blood brain barrier (BBB) permeability in surviving rats, leading to HT, increasement of brain edema, and exacerbation of neurological outcomes. Such deleterious effects of t-PA were dose-dependently and significantly attenuated by baicalin co-treatment. Delayed t-PA treatment increased 3-NT (a biomarker of peroxynitrite) formation, leading to MMP-9 overproduction and tight junction ZO-1 reduction, which were also significantly reversed by baicalin co-treatment. Taken together, baicalin could be a potential combination compound to reduce HT and other complications induced by delayed t-PA treatment, possibly via inhibiting peroxynitrite mediated MMP-9 production.
Copyright © 2016 Published by Elsevier Inc. |
Persistent Identifier | http://hdl.handle.net/10722/239472 |
ISSN | 2023 Impact Factor: 7.1 2023 SCImago Journal Rankings: 1.752 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Chen, H | - |
dc.contributor.author | Shen, J | - |
dc.date.accessioned | 2017-03-21T03:19:36Z | - |
dc.date.available | 2017-03-21T03:19:36Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | SfRBM 2016 & SFRRI 18th Biennial Meeting, San Francisco, CA., 16-19 November 2016. In Free Radical Biology and Medicine, 2016, v. 100 suppl., p. S159, abstract no. 376 | - |
dc.identifier.issn | 0891-5849 | - |
dc.identifier.uri | http://hdl.handle.net/10722/239472 | - |
dc.description.abstract | Hemorrhagic transformation (HT) is the severe complication of delayed tissue plasminogen activator (t-PA) treatment in ischemic stroke. Previously we showed that targeting peroxynitrite attenuated HT and improved neurological outcomes in ischemic stroke with thrombolytic therapy. Here we test the hypothesis that baicalin, a natural compound capable of scavenging peroxynitrite, could reduce HT in a rat cerebral ischemia-reperfusion model with delayed t-PA treatment, through targeting peroxynitrite-MMP-9 signaling. Male Sprague-Dawley (SD) rats were subjected to middle cerebral artery occlusion (MCAO) 5 hours plus reperfusion 19 hours. T-PA (10 mg/kg) or t-PA plus baicalin (50, 100, 150 mg/kg) was administrated via femoral vein at 4.5 hours after MCAO. Delayed t-PA treatment increased the mortality rates and further exacerbated blood brain barrier (BBB) permeability in surviving rats, leading to HT, increasement of brain edema, and exacerbation of neurological outcomes. Such deleterious effects of t-PA were dose-dependently and significantly attenuated by baicalin co-treatment. Delayed t-PA treatment increased 3-NT (a biomarker of peroxynitrite) formation, leading to MMP-9 overproduction and tight junction ZO-1 reduction, which were also significantly reversed by baicalin co-treatment. Taken together, baicalin could be a potential combination compound to reduce HT and other complications induced by delayed t-PA treatment, possibly via inhibiting peroxynitrite mediated MMP-9 production. Copyright © 2016 Published by Elsevier Inc. | - |
dc.language | eng | - |
dc.publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/freeradbiomed | - |
dc.relation.ispartof | Free Radical Biology & Medicine | - |
dc.rights | Posting accepted manuscript (postprint): © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | - |
dc.title | Baicalin reduces hemorrhagic transformation of rat ischemic stroke with delayed T-PA treatment | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Chen, H: chenhs@hku.hk | - |
dc.identifier.email | Shen, J: shenjg@hku.hk | - |
dc.identifier.authority | Shen, J=rp00487 | - |
dc.identifier.doi | 10.1016/j.freeradbiomed.2016.10.417 | - |
dc.identifier.hkuros | 271681 | - |
dc.identifier.volume | 100 | - |
dc.identifier.issue | suppl. | - |
dc.identifier.spage | S159, abstract no. 376 | - |
dc.identifier.epage | S159, abstract no. 376 | - |
dc.identifier.isi | WOS:000444355800377 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0891-5849 | - |