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- Publisher Website: 10.1016/j.jviromet.2010.01.015
- Scopus: eid_2-s2.0-77951024413
- PMID: 20117137
- WOS: WOS:000277763700008
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Article: Typing of human and animal strains of influenza virus with conserved signature peptides of matrix M1 protein by high resolution mass spectrometry
Title | Typing of human and animal strains of influenza virus with conserved signature peptides of matrix M1 protein by high resolution mass spectrometry |
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Authors | |
Keywords | Human Influenza virus Type Proteomics Mass spectrometry Animal Flu Matrix protein |
Issue Date | 2010 |
Citation | Journal of Virological Methods, 2010, v. 165, n. 2, p. 178-185 How to Cite? |
Abstract | The use of high resolution mass spectrometry to detect signature peptides within proteolytic digests of the isolated matrix M1 protein, and whole virus digests, for both human and animal strains of influenza is shown to be able to rapidly and reliably type the virus. Conserved sequences for predicted tryptic peptides were identified through alignments of matrix M1 protein sequences across all human, avian and swine strains of the influenza virus. Peptides with unique masses, when compared with those from the in silico digestion of all influenza antigens and those proteins known to contaminate egg grown strains, were identified using the purpose built FluGest algorithm. Their frequency of occurrence within the matrix M1 protein across all type A and type B strains was established with the FluAlign algorithm. The subsequent detection of the signature peptides of matrix M1 protein within proteolytic digests of type A and type B human and avian strains has been demonstrated. © 2010 Elsevier B.V. |
Persistent Identifier | http://hdl.handle.net/10722/250936 |
ISSN | 2023 Impact Factor: 2.2 2023 SCImago Journal Rankings: 0.510 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Schwahn, Alexander B. | - |
dc.contributor.author | Wong, Jason W.H. | - |
dc.contributor.author | Downard, Kevin M. | - |
dc.date.accessioned | 2018-02-01T01:54:07Z | - |
dc.date.available | 2018-02-01T01:54:07Z | - |
dc.date.issued | 2010 | - |
dc.identifier.citation | Journal of Virological Methods, 2010, v. 165, n. 2, p. 178-185 | - |
dc.identifier.issn | 0166-0934 | - |
dc.identifier.uri | http://hdl.handle.net/10722/250936 | - |
dc.description.abstract | The use of high resolution mass spectrometry to detect signature peptides within proteolytic digests of the isolated matrix M1 protein, and whole virus digests, for both human and animal strains of influenza is shown to be able to rapidly and reliably type the virus. Conserved sequences for predicted tryptic peptides were identified through alignments of matrix M1 protein sequences across all human, avian and swine strains of the influenza virus. Peptides with unique masses, when compared with those from the in silico digestion of all influenza antigens and those proteins known to contaminate egg grown strains, were identified using the purpose built FluGest algorithm. Their frequency of occurrence within the matrix M1 protein across all type A and type B strains was established with the FluAlign algorithm. The subsequent detection of the signature peptides of matrix M1 protein within proteolytic digests of type A and type B human and avian strains has been demonstrated. © 2010 Elsevier B.V. | - |
dc.language | eng | - |
dc.relation.ispartof | Journal of Virological Methods | - |
dc.subject | Human | - |
dc.subject | Influenza virus | - |
dc.subject | Type | - |
dc.subject | Proteomics | - |
dc.subject | Mass spectrometry | - |
dc.subject | Animal | - |
dc.subject | Flu | - |
dc.subject | Matrix protein | - |
dc.title | Typing of human and animal strains of influenza virus with conserved signature peptides of matrix M1 protein by high resolution mass spectrometry | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.jviromet.2010.01.015 | - |
dc.identifier.pmid | 20117137 | - |
dc.identifier.scopus | eid_2-s2.0-77951024413 | - |
dc.identifier.volume | 165 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | 178 | - |
dc.identifier.epage | 185 | - |
dc.identifier.isi | WOS:000277763700008 | - |
dc.identifier.issnl | 0166-0934 | - |