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Conference Paper: New Mechanistic Insights into PARP-1-mediated DNA Damage Response
Title | New Mechanistic Insights into PARP-1-mediated DNA Damage Response |
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Authors | |
Issue Date | 2016 |
Citation | Seminar, Department of Biomedical Sciences, City University of Hong Kong, Hong Kong,15 December 2016 How to Cite? |
Abstract | PARP-1 is a major poly(ADP-ribose) transferase that plays an important role in DNA damage repair. PARP-1 has been shown to be a DNA single-strand and double-strand break sensor protein that helps rapidly recruit many downstream DNA repair proteins to damaged DNA sites in a poly(ADP-ribose) dependent manner. In this presentation I will talk about a new mechanism of PARP-1-mediated DNA damage response. We identified and provided the biochemical and structural evidence that PARP-1 interacts with a critical protein called Timeless. We demonstrated that rapid and transient accumulation of Timeless at laser-induced DNA damage sites requires PARP-1 but not poly(ADP-ribosyl)ation and that Timeless is co-trapped with PARP-1 at DNA lesions upon PARP inhibition. Furthermore, we show that Timeless and PARP-1 interaction is required for efficient homologous recombination repair. |
Persistent Identifier | http://hdl.handle.net/10722/252983 |
DC Field | Value | Language |
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dc.contributor.author | Qian, C | - |
dc.date.accessioned | 2018-05-09T07:24:56Z | - |
dc.date.available | 2018-05-09T07:24:56Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | Seminar, Department of Biomedical Sciences, City University of Hong Kong, Hong Kong,15 December 2016 | - |
dc.identifier.uri | http://hdl.handle.net/10722/252983 | - |
dc.description.abstract | PARP-1 is a major poly(ADP-ribose) transferase that plays an important role in DNA damage repair. PARP-1 has been shown to be a DNA single-strand and double-strand break sensor protein that helps rapidly recruit many downstream DNA repair proteins to damaged DNA sites in a poly(ADP-ribose) dependent manner. In this presentation I will talk about a new mechanism of PARP-1-mediated DNA damage response. We identified and provided the biochemical and structural evidence that PARP-1 interacts with a critical protein called Timeless. We demonstrated that rapid and transient accumulation of Timeless at laser-induced DNA damage sites requires PARP-1 but not poly(ADP-ribosyl)ation and that Timeless is co-trapped with PARP-1 at DNA lesions upon PARP inhibition. Furthermore, we show that Timeless and PARP-1 interaction is required for efficient homologous recombination repair. | - |
dc.language | eng | - |
dc.relation.ispartof | City University of Hong Kong, Department of Biomedical Sciences, Seminar | - |
dc.title | New Mechanistic Insights into PARP-1-mediated DNA Damage Response | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Qian, C: cmqian@hku.hk | - |
dc.identifier.authority | Qian, C=rp01371 | - |
dc.identifier.hkuros | 280806 | - |