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- Publisher Website: 10.1049/mnl.2017.0348
- Scopus: eid_2-s2.0-85045124864
- WOS: WOS:000429200100001
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Article: Multi-Binding Biotinylated Iron Oxide Nanoparticles (biotin-IONPs) as a Promising Versatile Material for Magnetic Biomedical Applications
Title | Multi-Binding Biotinylated Iron Oxide Nanoparticles (biotin-IONPs) as a Promising Versatile Material for Magnetic Biomedical Applications |
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Authors | |
Keywords | Biomedical materials Biomedical MRI Drug delivery systems Drugs Iron compounds |
Issue Date | 2018 |
Publisher | The Institution of Engineering and Technology. The Journal's web site is located at http://www.ietdl.org/MNL |
Citation | Micro and Nano Letters, 2018, v. 13 n. 4, p. 415-420 How to Cite? |
Abstract | The tetrameric streptavidin and avidin both have four sites that can specifically bind with biotin. Taking advantage of this high affinity binding, it is possible to realise multi-binding of biotinylated nanoparticles to avidin or streptavidin molecules. The iron oxide nanoparticles (IONPs) have been actively investigated in biomedical applications. In this work, biotinylated IONPs (biotin-IONPs) with around 10.5 nm magnetic core size were synthesised. They exhibited long-term hydrophilic stability, and the activity of the biotin content was verified by avidin-horseradish peroxidase induced chromogenic experiment. These biotin-IONPs were then studied as magnetic labels in substrate-based and substrate-free magnetic bio-labelling scheme of alpha-fetoprotein. The multi-binding ability of these biotin-IONPs with streptavidin functionalised detection antibody (streptavidin-detection antibody) was proved both on microplate well surfaces and in solution. Consequently, biotin-IONPs are promising magnetic label materials for the improvement of magnetic biodetection. In addition, the avidin-induced biotin-IONP assemblies were produced in MilliQ water solution and in water-in-oil microemulsion system. In summary, based on their multi-binding nature, the biotin-IONPs can be considered as a versatile material to improve the sensitivity of magnetic biodetection and to be further built into assemblies for future biomedical applications such as magnetic resonance imaging and target drug delivery. |
Persistent Identifier | http://hdl.handle.net/10722/259268 |
ISSN | 2023 Impact Factor: 1.5 2023 SCImago Journal Rankings: 0.262 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Du, Y | - |
dc.contributor.author | Pong, PWT | - |
dc.date.accessioned | 2018-09-03T04:04:09Z | - |
dc.date.available | 2018-09-03T04:04:09Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | Micro and Nano Letters, 2018, v. 13 n. 4, p. 415-420 | - |
dc.identifier.issn | 1750-0443 | - |
dc.identifier.uri | http://hdl.handle.net/10722/259268 | - |
dc.description.abstract | The tetrameric streptavidin and avidin both have four sites that can specifically bind with biotin. Taking advantage of this high affinity binding, it is possible to realise multi-binding of biotinylated nanoparticles to avidin or streptavidin molecules. The iron oxide nanoparticles (IONPs) have been actively investigated in biomedical applications. In this work, biotinylated IONPs (biotin-IONPs) with around 10.5 nm magnetic core size were synthesised. They exhibited long-term hydrophilic stability, and the activity of the biotin content was verified by avidin-horseradish peroxidase induced chromogenic experiment. These biotin-IONPs were then studied as magnetic labels in substrate-based and substrate-free magnetic bio-labelling scheme of alpha-fetoprotein. The multi-binding ability of these biotin-IONPs with streptavidin functionalised detection antibody (streptavidin-detection antibody) was proved both on microplate well surfaces and in solution. Consequently, biotin-IONPs are promising magnetic label materials for the improvement of magnetic biodetection. In addition, the avidin-induced biotin-IONP assemblies were produced in MilliQ water solution and in water-in-oil microemulsion system. In summary, based on their multi-binding nature, the biotin-IONPs can be considered as a versatile material to improve the sensitivity of magnetic biodetection and to be further built into assemblies for future biomedical applications such as magnetic resonance imaging and target drug delivery. | - |
dc.language | eng | - |
dc.publisher | The Institution of Engineering and Technology. The Journal's web site is located at http://www.ietdl.org/MNL | - |
dc.relation.ispartof | Micro and Nano Letters | - |
dc.subject | Biomedical materials | - |
dc.subject | Biomedical MRI | - |
dc.subject | Drug delivery systems | - |
dc.subject | Drugs | - |
dc.subject | Iron compounds | - |
dc.title | Multi-Binding Biotinylated Iron Oxide Nanoparticles (biotin-IONPs) as a Promising Versatile Material for Magnetic Biomedical Applications | - |
dc.type | Article | - |
dc.identifier.email | Pong, PWT: ppong@hkucc.hku.hk | - |
dc.identifier.authority | Pong, PWT=rp00217 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1049/mnl.2017.0348 | - |
dc.identifier.scopus | eid_2-s2.0-85045124864 | - |
dc.identifier.hkuros | 288671 | - |
dc.identifier.volume | 13 | - |
dc.identifier.issue | 4 | - |
dc.identifier.spage | 415 | - |
dc.identifier.epage | 420 | - |
dc.identifier.isi | WOS:000429200100001 | - |
dc.publisher.place | United Kingdom | - |
dc.identifier.issnl | 1750-0443 | - |