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Conference Paper: The effect of 3-hydroxy-3-methylglutaryl-CoA synthase extends beyond the isoprenoid pathway
Title | The effect of 3-hydroxy-3-methylglutaryl-CoA synthase extends beyond the isoprenoid pathway |
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Authors | |
Issue Date | 2018 |
Publisher | Global Engage. |
Citation | 5th Plant Genomics & Gene Editing Congress Asia, Bangkok, Thailand, 26-27 March 2018 How to Cite? |
Abstract | The mevalonate (MVA) pathway generates isoprenoids including phytosterols. Dietary phytosterols are important because they can lower blood cholesterol levels. In the MVA pathway, 3-hydroxy-3-methylglutaryl-CoA synthase (HMGS) catalyses the condensation of acetoacetyl-CoA and acetyl-CoA to form HMG-CoA. Previous studies have shown that the recombinant Brassica juncea HMGS1 (BjHMGS1) mutant S359A displayed 10-fold higher enzyme activity than wild-type (wt) BjHMGS1 while transgenic tobacco S359A-overexpressors (OE-S359A) accumulated sterol content, and possessed higher growth rate and seed yield than OE-wtBjHMGS1. Herein, omics methods were employed to understand the differences brought about by HMGS overexpression. Comparative transcriptomics analysis verified
that the improved effect of OE-S359A resulted from an increase in downstream isoprenoid biosynthesis-, primary metabolismand plant hormone-related gene expression. Proteomics and metabolomics data will be discussed to demonstrate that HMGS links the MVA pathway to the central metabolic pathways. |
Description | Invited talk - Plant Engineering Technologies |
Persistent Identifier | http://hdl.handle.net/10722/267653 |
DC Field | Value | Language |
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dc.contributor.author | Liao, P | - |
dc.contributor.author | Chye, ML | - |
dc.date.accessioned | 2019-02-26T07:36:06Z | - |
dc.date.available | 2019-02-26T07:36:06Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | 5th Plant Genomics & Gene Editing Congress Asia, Bangkok, Thailand, 26-27 March 2018 | - |
dc.identifier.uri | http://hdl.handle.net/10722/267653 | - |
dc.description | Invited talk - Plant Engineering Technologies | - |
dc.description.abstract | The mevalonate (MVA) pathway generates isoprenoids including phytosterols. Dietary phytosterols are important because they can lower blood cholesterol levels. In the MVA pathway, 3-hydroxy-3-methylglutaryl-CoA synthase (HMGS) catalyses the condensation of acetoacetyl-CoA and acetyl-CoA to form HMG-CoA. Previous studies have shown that the recombinant Brassica juncea HMGS1 (BjHMGS1) mutant S359A displayed 10-fold higher enzyme activity than wild-type (wt) BjHMGS1 while transgenic tobacco S359A-overexpressors (OE-S359A) accumulated sterol content, and possessed higher growth rate and seed yield than OE-wtBjHMGS1. Herein, omics methods were employed to understand the differences brought about by HMGS overexpression. Comparative transcriptomics analysis verified that the improved effect of OE-S359A resulted from an increase in downstream isoprenoid biosynthesis-, primary metabolismand plant hormone-related gene expression. Proteomics and metabolomics data will be discussed to demonstrate that HMGS links the MVA pathway to the central metabolic pathways. | - |
dc.language | eng | - |
dc.publisher | Global Engage. | - |
dc.relation.ispartof | 5th Plant Genomics & Gene Editing Congress Asia, 2018 | - |
dc.title | The effect of 3-hydroxy-3-methylglutaryl-CoA synthase extends beyond the isoprenoid pathway | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Liao, P: h1092030@connect.hku.hk | - |
dc.identifier.email | Chye, ML: mlchye@hku.hk | - |
dc.identifier.authority | Chye, ML=rp00687 | - |
dc.identifier.hkuros | 290342 | - |
dc.publisher.place | Malaysia | - |