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- Publisher Website: 10.1016/j.xpro.2019.100011
- Scopus: eid_2-s2.0-85101267327
- PMID: 32783031
- WOS: WOS:001050081900017
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Article: Purification of Affinity Tag-free Recombinant Tubulin from Insect Cells
Title | Purification of Affinity Tag-free Recombinant Tubulin from Insect Cells |
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Authors | |
Issue Date | 2020 |
Publisher | Elsevier (Cell Press): OAJ. The Journal's web site is located at https://www.cell.com/star-protocols/home |
Citation | STAR Protocols, 2020, v. 1, p. article no. 100011 How to Cite? |
Abstract | α/β-tubulin heterodimers, which can harbor diverse isotypes and post-translational modifications, polymerize into microtubules that are fundamental to many cellular processes. Due to long-standing challenges in generating recombinant tubulin, however, it has been difficult to examine the properties of specific tubulin isotypes. Here, we provide a protocol for purifying milligrams of affinity tag-free, isotypically pure recombinant tubulin. Our method can be applicable to any tubulin of interest, opening the door to dissecting how tubulin diversity regulates microtubule function. |
Persistent Identifier | http://hdl.handle.net/10722/284516 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Ti, SC | - |
dc.contributor.author | Wieczorek, M | - |
dc.contributor.author | Kapoor, TM | - |
dc.date.accessioned | 2020-08-07T08:58:46Z | - |
dc.date.available | 2020-08-07T08:58:46Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | STAR Protocols, 2020, v. 1, p. article no. 100011 | - |
dc.identifier.uri | http://hdl.handle.net/10722/284516 | - |
dc.description.abstract | α/β-tubulin heterodimers, which can harbor diverse isotypes and post-translational modifications, polymerize into microtubules that are fundamental to many cellular processes. Due to long-standing challenges in generating recombinant tubulin, however, it has been difficult to examine the properties of specific tubulin isotypes. Here, we provide a protocol for purifying milligrams of affinity tag-free, isotypically pure recombinant tubulin. Our method can be applicable to any tubulin of interest, opening the door to dissecting how tubulin diversity regulates microtubule function. | - |
dc.language | eng | - |
dc.publisher | Elsevier (Cell Press): OAJ. The Journal's web site is located at https://www.cell.com/star-protocols/home | - |
dc.relation.ispartof | STAR Protocols | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.title | Purification of Affinity Tag-free Recombinant Tubulin from Insect Cells | - |
dc.type | Article | - |
dc.identifier.email | Ti, SC: jeffti@hku.hk | - |
dc.identifier.authority | Ti, SC=rp02617 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1016/j.xpro.2019.100011 | - |
dc.identifier.pmid | 32783031 | - |
dc.identifier.pmcid | PMC7416841 | - |
dc.identifier.scopus | eid_2-s2.0-85101267327 | - |
dc.identifier.hkuros | 311691 | - |
dc.identifier.volume | 1 | - |
dc.identifier.spage | article no. 100011 | - |
dc.identifier.epage | article no. 100011 | - |
dc.identifier.eissn | 2666-1667 | - |
dc.identifier.isi | WOS:001050081900017 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 2666-1667 | - |