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Conference Paper: Vitamin D, a novel treatment for intracerebral hemorrhage: a preclinical study
Title | Vitamin D, a novel treatment for intracerebral hemorrhage: a preclinical study |
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Authors | |
Issue Date | 2020 |
Publisher | The Hong Kong Neurosurgical Society. |
Citation | 27th Annual Scientific Meeting of The Hong Kong Neurosurgical Society: Smart Hospital and Artificial Intelligence in Neurosurgery, Virtual Conference, Hong Kong, 18-19 December 2020 How to Cite? |
Abstract | Objective: Intracerebral hemorrhage (ICH) is second deadly CNS diseases with high mortality rate up to 60%. Current treatments for ICH are limited in therapeutic significant and resulted in poor prognosis. Studies in recent decades revealed that Vitamin D (Vit D) would significantly benefit in ischemic stroke. However, actions of Vit D in ICH have yet been explored. Our study is aimed to fill this knowledge gap.
Method: We employed a standardized collagenase-induced ICH model in male mice. 1000IU/kg/day of
Vit D were given to post-ICH mice orally with Vit D normal and Vit D deficient status for 4 weeks. To evaluate the therapeutic effects of vitamin D in ICH, three neurofunctional assessments were performed every 7 days after ICH. Underline pathophysiological mechanisms in neuronal survival, astrogliosis and myelination were investigated with histology and western blot.
Result: Overall, significantly better functional outcomes were achieved with Vit D treatment mice. We recorded that higher mortality in Vit D deficient group and measured with 12% larger in hematoma volume. Immunohistological staining to the brain slides on 28th day: (i) more survived neurons and less reactive astrocytes were found in the peri-lesion area in Vit D treatment group; (ii) higher expression level of neuronal myelination markers were found in Vit D treatment. Western bolt studies confirmed the above findings.
Conclusion: These results indicate that Vit D may serve as neuroprotective agent in promoting neurofunctional recovery after ICH. Actions of Vit D in ICH are: (i) promoted neuronal survival; (ii) reduced proliferation of reactive astrocytes; (iii) enhanced neuronal myelination.
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Description | Oral presentation - Free Paper VII |
Persistent Identifier | http://hdl.handle.net/10722/295809 |
DC Field | Value | Language |
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dc.contributor.author | Cheng, Y | - |
dc.contributor.author | Leung, GKK | - |
dc.date.accessioned | 2021-02-08T08:14:19Z | - |
dc.date.available | 2021-02-08T08:14:19Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | 27th Annual Scientific Meeting of The Hong Kong Neurosurgical Society: Smart Hospital and Artificial Intelligence in Neurosurgery, Virtual Conference, Hong Kong, 18-19 December 2020 | - |
dc.identifier.uri | http://hdl.handle.net/10722/295809 | - |
dc.description | Oral presentation - Free Paper VII | - |
dc.description.abstract | Objective: Intracerebral hemorrhage (ICH) is second deadly CNS diseases with high mortality rate up to 60%. Current treatments for ICH are limited in therapeutic significant and resulted in poor prognosis. Studies in recent decades revealed that Vitamin D (Vit D) would significantly benefit in ischemic stroke. However, actions of Vit D in ICH have yet been explored. Our study is aimed to fill this knowledge gap. Method: We employed a standardized collagenase-induced ICH model in male mice. 1000IU/kg/day of Vit D were given to post-ICH mice orally with Vit D normal and Vit D deficient status for 4 weeks. To evaluate the therapeutic effects of vitamin D in ICH, three neurofunctional assessments were performed every 7 days after ICH. Underline pathophysiological mechanisms in neuronal survival, astrogliosis and myelination were investigated with histology and western blot. Result: Overall, significantly better functional outcomes were achieved with Vit D treatment mice. We recorded that higher mortality in Vit D deficient group and measured with 12% larger in hematoma volume. Immunohistological staining to the brain slides on 28th day: (i) more survived neurons and less reactive astrocytes were found in the peri-lesion area in Vit D treatment group; (ii) higher expression level of neuronal myelination markers were found in Vit D treatment. Western bolt studies confirmed the above findings. Conclusion: These results indicate that Vit D may serve as neuroprotective agent in promoting neurofunctional recovery after ICH. Actions of Vit D in ICH are: (i) promoted neuronal survival; (ii) reduced proliferation of reactive astrocytes; (iii) enhanced neuronal myelination. | - |
dc.language | eng | - |
dc.publisher | The Hong Kong Neurosurgical Society. | - |
dc.relation.ispartof | 27th Annual Scientific Meeting (Virtual Conference) of The Hong Kong Neurosurgical Society, 2020 | - |
dc.title | Vitamin D, a novel treatment for intracerebral hemorrhage: a preclinical study | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Leung, GKK: gkkleung@hku.hk | - |
dc.identifier.authority | Leung, GKK=rp00522 | - |
dc.identifier.hkuros | 321108 | - |
dc.publisher.place | Hong Kong | - |