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Article: Regulation of macrophage polarization through surface topography design to facilitate implant-to-bone osteointegration
Title | Regulation of macrophage polarization through surface topography design to facilitate implant-to-bone osteointegration |
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Authors | |
Issue Date | 2021 |
Publisher | American Association for the Advancement of Science. The Journal's web site is located at http://www.scienceadvances.org/ |
Citation | Science Advances, 2021, v. 7 n. 14, p. eabf6654 How to Cite? |
Abstract | Proper immune responses are critical for successful biomaterial implantation. Here, four scales of honeycomb-like TiO2 structures were custom made on titanium (Ti) substrates to investigate cellular behaviors of RAW 264.7 macrophages and their immunomodulation on osteogenesis. We found that the reduced scale of honeycomb-like TiO2 structures could significantly activate the anti-inflammatory macrophage phenotype (M2), in which the 90-nanometer sample induced the highest expression level of CD206, interleukin-4, and interleukin-10 and released the highest amount of bone morphogenetic protein-2 among other scales. Afterward, the resulting immune microenvironment favorably triggered osteogenic differentiation of murine mesenchymal stem cells in vitro and subsequent implant-to-bone osteointegration in vivo. Furthermore, transcriptomic analysis revealed that the minimal scale of TiO2 honeycomb-like structure (90 nanometers) facilitated macrophage filopodia formation and up-regulated the Rho family of guanosine triphosphatases (RhoA, Rac1, and CDC42), which reinforced the polarization of macrophages through the activation of the RhoA/Rho-associated protein kinase signaling pathway. |
Persistent Identifier | http://hdl.handle.net/10722/302352 |
ISSN | 2021 Impact Factor: 14.957 2020 SCImago Journal Rankings: 5.928 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Zhu, Y | - |
dc.contributor.author | Liang, H | - |
dc.contributor.author | Liy, X | - |
dc.contributor.author | Wu, J | - |
dc.contributor.author | Yang, C | - |
dc.contributor.author | Wong, TM | - |
dc.contributor.author | Kwan, KYH | - |
dc.contributor.author | Cheung, KMC | - |
dc.contributor.author | Wu, S | - |
dc.contributor.author | Yeung, KWK | - |
dc.date.accessioned | 2021-09-06T03:31:03Z | - |
dc.date.available | 2021-09-06T03:31:03Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Science Advances, 2021, v. 7 n. 14, p. eabf6654 | - |
dc.identifier.issn | 2375-2548 | - |
dc.identifier.uri | http://hdl.handle.net/10722/302352 | - |
dc.description.abstract | Proper immune responses are critical for successful biomaterial implantation. Here, four scales of honeycomb-like TiO2 structures were custom made on titanium (Ti) substrates to investigate cellular behaviors of RAW 264.7 macrophages and their immunomodulation on osteogenesis. We found that the reduced scale of honeycomb-like TiO2 structures could significantly activate the anti-inflammatory macrophage phenotype (M2), in which the 90-nanometer sample induced the highest expression level of CD206, interleukin-4, and interleukin-10 and released the highest amount of bone morphogenetic protein-2 among other scales. Afterward, the resulting immune microenvironment favorably triggered osteogenic differentiation of murine mesenchymal stem cells in vitro and subsequent implant-to-bone osteointegration in vivo. Furthermore, transcriptomic analysis revealed that the minimal scale of TiO2 honeycomb-like structure (90 nanometers) facilitated macrophage filopodia formation and up-regulated the Rho family of guanosine triphosphatases (RhoA, Rac1, and CDC42), which reinforced the polarization of macrophages through the activation of the RhoA/Rho-associated protein kinase signaling pathway. | - |
dc.language | eng | - |
dc.publisher | American Association for the Advancement of Science. The Journal's web site is located at http://www.scienceadvances.org/ | - |
dc.relation.ispartof | Science Advances | - |
dc.rights | Science Advances. Copyright © American Association for the Advancement of Science. | - |
dc.title | Regulation of macrophage polarization through surface topography design to facilitate implant-to-bone osteointegration | - |
dc.type | Article | - |
dc.identifier.email | Wong, TM: wongtm@hku.hk | - |
dc.identifier.email | Kwan, KYH: kyhkwan@hku.hk | - |
dc.identifier.email | Cheung, KMC: cheungmc@hku.hk | - |
dc.identifier.email | Yeung, KWK: wkkyeung@hku.hk | - |
dc.identifier.authority | Wong, TM=rp01689 | - |
dc.identifier.authority | Kwan, KYH=rp02014 | - |
dc.identifier.authority | Cheung, KMC=rp00387 | - |
dc.identifier.authority | Yeung, KWK=rp00309 | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1126/sciadv.abf6654 | - |
dc.identifier.hkuros | 324729 | - |
dc.identifier.volume | 7 | - |
dc.identifier.issue | 14 | - |
dc.identifier.spage | eabf6654 | - |
dc.identifier.epage | eabf6654 | - |
dc.identifier.isi | WOS:000636455600032 | - |
dc.publisher.place | United States | - |