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- Publisher Website: 10.3389/fmicb.2021.748121
- Scopus: eid_2-s2.0-85118640052
- PMID: 34745052
- WOS: WOS:000716611100001
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Article: Eradication of Porphyromonas gingivalis Persisters Through Colloidal Bismuth Subcitrate Synergistically Combined With Metronidazole
Title | Eradication of Porphyromonas gingivalis Persisters Through Colloidal Bismuth Subcitrate Synergistically Combined With Metronidazole |
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Authors | |
Keywords | Porphyromonas gingivalis persisters eradication bismuth drugs metronidazole |
Issue Date | 2021 |
Publisher | Frontiers Research Foundation. The Journal's web site is located at http://www.frontiersin.org/microbiology/ |
Citation | Frontiers in Microbiology, 2021, v. 12, p. article no. 748121 How to Cite? |
Abstract | Microbial persisters enable the development of certain intrinsic strategies for survival with extreme tolerance to multiple antimicrobials. Porphyromonas gingivalis is considered to be the “keystone” periodontopathogen. Indeed, periodontitis, as a highly common inflammatory disease, is the major cause of severe tooth loss and edentulism in adults globally, and yet it is crucially involved in various systemic comorbidities like diabetes. We have recently revealed P. gingivalis persisters-induced perturbation of immuno-inflammatory responses and effective suppression of this key pathogen by bismuth drugs. This study further explored novel approaches to eradicating P. gingivalis persisters through synergistic combination of colloidal bismuth subcitrate (CBS) with traditional antibiotics. P. gingivalis (ATCC 33277) cells in planktonic and biofilm states were cultured to stationary phase, and then treated with metronidazole (100 mg/L), amoxicillin (100 mg/L), CBS, (100 μM) and combinations of these medications, respectively. Persister survival rate was calculated by colony-forming unit. Cell viability and cytotoxicity of CBS were assessed in human gingival epithelial cells (HGECs). Notably, CBS combined with metronidazole enabled the effective eradication of P. gingivalis persisters in planktonic mode, and nearly eliminated their existence in biofilm mode. Importantly, CBS exhibited no effects on the viability of HGECs, along with minimal cytotoxicity (<5%) even at a high concentration (400 μM). This pioneering study shows that P. gingivalis persisters could be well eliminated via the synergistic combination of CBS with metronidazole. Our findings may contribute to developing novel approaches to tackling periodontitis and inflammatory systemic comorbidities. |
Persistent Identifier | http://hdl.handle.net/10722/306673 |
ISSN | 2023 Impact Factor: 4.0 2023 SCImago Journal Rankings: 1.065 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | WANG, C | - |
dc.contributor.author | Li, X | - |
dc.contributor.author | Cheng, T | - |
dc.contributor.author | Sun, H | - |
dc.contributor.author | Jin, L | - |
dc.date.accessioned | 2021-10-22T07:37:58Z | - |
dc.date.available | 2021-10-22T07:37:58Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Frontiers in Microbiology, 2021, v. 12, p. article no. 748121 | - |
dc.identifier.issn | 1664-302X | - |
dc.identifier.uri | http://hdl.handle.net/10722/306673 | - |
dc.description.abstract | Microbial persisters enable the development of certain intrinsic strategies for survival with extreme tolerance to multiple antimicrobials. Porphyromonas gingivalis is considered to be the “keystone” periodontopathogen. Indeed, periodontitis, as a highly common inflammatory disease, is the major cause of severe tooth loss and edentulism in adults globally, and yet it is crucially involved in various systemic comorbidities like diabetes. We have recently revealed P. gingivalis persisters-induced perturbation of immuno-inflammatory responses and effective suppression of this key pathogen by bismuth drugs. This study further explored novel approaches to eradicating P. gingivalis persisters through synergistic combination of colloidal bismuth subcitrate (CBS) with traditional antibiotics. P. gingivalis (ATCC 33277) cells in planktonic and biofilm states were cultured to stationary phase, and then treated with metronidazole (100 mg/L), amoxicillin (100 mg/L), CBS, (100 μM) and combinations of these medications, respectively. Persister survival rate was calculated by colony-forming unit. Cell viability and cytotoxicity of CBS were assessed in human gingival epithelial cells (HGECs). Notably, CBS combined with metronidazole enabled the effective eradication of P. gingivalis persisters in planktonic mode, and nearly eliminated their existence in biofilm mode. Importantly, CBS exhibited no effects on the viability of HGECs, along with minimal cytotoxicity (<5%) even at a high concentration (400 μM). This pioneering study shows that P. gingivalis persisters could be well eliminated via the synergistic combination of CBS with metronidazole. Our findings may contribute to developing novel approaches to tackling periodontitis and inflammatory systemic comorbidities. | - |
dc.language | eng | - |
dc.publisher | Frontiers Research Foundation. The Journal's web site is located at http://www.frontiersin.org/microbiology/ | - |
dc.relation.ispartof | Frontiers in Microbiology | - |
dc.rights | This Document is Protected by copyright and was first published by Frontiers. All rights reserved. It is reproduced with permission. | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | Porphyromonas gingivalis | - |
dc.subject | persisters | - |
dc.subject | eradication | - |
dc.subject | bismuth drugs | - |
dc.subject | metronidazole | - |
dc.title | Eradication of Porphyromonas gingivalis Persisters Through Colloidal Bismuth Subcitrate Synergistically Combined With Metronidazole | - |
dc.type | Article | - |
dc.identifier.email | Li, X: llx815@hku.hk | - |
dc.identifier.email | Cheng, T: chengtfc@hku.hk | - |
dc.identifier.email | Sun, H: hsun@hku.hk | - |
dc.identifier.email | Jin, L: ljjin@hkucc.hku.hk | - |
dc.identifier.authority | Li, X=rp02494 | - |
dc.identifier.authority | Sun, H=rp00777 | - |
dc.identifier.authority | Jin, L=rp00028 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.3389/fmicb.2021.748121 | - |
dc.identifier.pmid | 34745052 | - |
dc.identifier.pmcid | PMC8565575 | - |
dc.identifier.scopus | eid_2-s2.0-85118640052 | - |
dc.identifier.hkuros | 329205 | - |
dc.identifier.volume | 12 | - |
dc.identifier.spage | article no. 748121 | - |
dc.identifier.epage | article no. 748121 | - |
dc.identifier.isi | WOS:000716611100001 | - |
dc.publisher.place | Switzerland | - |