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- Publisher Website: 10.1083/jcb.200801022
- Scopus: eid_2-s2.0-51649088232
- PMID: 18762577
- WOS: WOS:000259050700017
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Article: TLR ligand-induced podosome disassembly in dendritic cells is ADAM17 dependent
Title | TLR ligand-induced podosome disassembly in dendritic cells is ADAM17 dependent | ||||||
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Authors | |||||||
Issue Date | 2008 | ||||||
Publisher | Rockefeller University Press. The Journal's web site is located at http://www.jcb.org | ||||||
Citation | Journal Of Cell Biology, 2008, v. 182 n. 5, p. 993-1005 How to Cite? | ||||||
Abstract | Toll-like receptor (TLR) signaling induces a rapid reorganization of the actin cytoskeleton in cultured mouse dendritic cells (DC), leading to enhanced antigen endocytosis and a concomitant loss of filamentous actin-rich podosomes. We show that as podosomes are lost, TLR signaling induces prominent focal contacts and a transient reduction in DC migratory capacity in vitro. We further show that podosomes in mouse DC are foci of pronounced gelatinase activity, dependent on the enzyme membrane type I matrix metalloprotease (MT1-MMP), and that DC transiently lose the ability to degrade the extracellular matrix after TLR signaling. Surprisingly, MMP inhibitors block TLR signaling-induced podosome disassembly, although stimulated endocytosis is unaffected, which demonstrates that the two phenomena are not obligatorily coupled. Podosome disassembly caused by TLR signaling occurs normally in DC lacking MT1-MMP, and instead requires the tumor necrosis factor α-converting enzyme ADAM17 (a disintegrin and metalloprotease 17), which demonstrates a novel role for this sheddase in regulating an actin-based structure. © 2008 West et al. | ||||||
Persistent Identifier | http://hdl.handle.net/10722/58262 | ||||||
ISSN | 2023 Impact Factor: 7.4 2023 SCImago Journal Rankings: 3.717 | ||||||
ISI Accession Number ID |
Funding Information: We are grateful to the Medical Research Council for program grant GO200536 (to C. Watts and A. R. Prescott) and the Hong Kong Research Grant Council (grant CERG 751303 to Z. Zhou) for funding. | ||||||
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | West, MA | en_HK |
dc.contributor.author | Prescott, AR | en_HK |
dc.contributor.author | Kui, MC | en_HK |
dc.contributor.author | Zhou, Z | en_HK |
dc.contributor.author | RoseJohn, S | en_HK |
dc.contributor.author | Scheller, J | en_HK |
dc.contributor.author | Watts, C | en_HK |
dc.date.accessioned | 2010-05-31T03:27:00Z | - |
dc.date.available | 2010-05-31T03:27:00Z | - |
dc.date.issued | 2008 | en_HK |
dc.identifier.citation | Journal Of Cell Biology, 2008, v. 182 n. 5, p. 993-1005 | en_HK |
dc.identifier.issn | 0021-9525 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/58262 | - |
dc.description.abstract | Toll-like receptor (TLR) signaling induces a rapid reorganization of the actin cytoskeleton in cultured mouse dendritic cells (DC), leading to enhanced antigen endocytosis and a concomitant loss of filamentous actin-rich podosomes. We show that as podosomes are lost, TLR signaling induces prominent focal contacts and a transient reduction in DC migratory capacity in vitro. We further show that podosomes in mouse DC are foci of pronounced gelatinase activity, dependent on the enzyme membrane type I matrix metalloprotease (MT1-MMP), and that DC transiently lose the ability to degrade the extracellular matrix after TLR signaling. Surprisingly, MMP inhibitors block TLR signaling-induced podosome disassembly, although stimulated endocytosis is unaffected, which demonstrates that the two phenomena are not obligatorily coupled. Podosome disassembly caused by TLR signaling occurs normally in DC lacking MT1-MMP, and instead requires the tumor necrosis factor α-converting enzyme ADAM17 (a disintegrin and metalloprotease 17), which demonstrates a novel role for this sheddase in regulating an actin-based structure. © 2008 West et al. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Rockefeller University Press. The Journal's web site is located at http://www.jcb.org | en_HK |
dc.relation.ispartof | Journal of Cell Biology | en_HK |
dc.rights | Journal of Cellular Biochemistry. Copyright © John Wiley & Sons, Inc. | en_HK |
dc.subject.mesh | ADAM Proteins - metabolism - physiology | en_HK |
dc.subject.mesh | Actin Cytoskeleton - metabolism - ultrastructure | en_HK |
dc.subject.mesh | Actins - metabolism | en_HK |
dc.subject.mesh | Animals | en_HK |
dc.subject.mesh | Cell Movement | en_HK |
dc.subject.mesh | Dendritic Cells - enzymology - metabolism - ultrastructure | en_HK |
dc.subject.mesh | Extracellular Matrix - metabolism | en_HK |
dc.subject.mesh | Female | en_HK |
dc.subject.mesh | Gelatinases - metabolism | en_HK |
dc.subject.mesh | Ligands | en_HK |
dc.subject.mesh | Matrix Metalloproteinases - antagonists & inhibitors - metabolism | en_HK |
dc.subject.mesh | Mice | en_HK |
dc.subject.mesh | Mice, Inbred Strains | en_HK |
dc.subject.mesh | Pinocytosis - drug effects | en_HK |
dc.subject.mesh | Protease Inhibitors - pharmacology | en_HK |
dc.subject.mesh | Signal Transduction - drug effects | en_HK |
dc.subject.mesh | Toll-Like Receptors - metabolism | en_HK |
dc.title | TLR ligand-induced podosome disassembly in dendritic cells is ADAM17 dependent | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0730-2312&volume=182&spage=993&epage=1005&date=2008&atitle=Tlr+Ligand-induced+Podosome+Disassembly+In+Dendritic+Cells+Is+Adam17+Dependent | en_HK |
dc.identifier.email | Zhou, Z:zhongjun@hkucc.hku.hk | en_HK |
dc.identifier.authority | Zhou, Z=rp00503 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1083/jcb.200801022 | en_HK |
dc.identifier.pmid | 18762577 | en_HK |
dc.identifier.scopus | eid_2-s2.0-51649088232 | en_HK |
dc.identifier.hkuros | 153649 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-51649088232&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 182 | en_HK |
dc.identifier.issue | 5 | en_HK |
dc.identifier.spage | 993 | en_HK |
dc.identifier.epage | 1005 | en_HK |
dc.identifier.isi | WOS:000259050700017 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | West, MA=35605973600 | en_HK |
dc.identifier.scopusauthorid | Prescott, AR=7005983965 | en_HK |
dc.identifier.scopusauthorid | Kui, MC=24780760100 | en_HK |
dc.identifier.scopusauthorid | Zhou, Z=8631856300 | en_HK |
dc.identifier.scopusauthorid | RoseJohn, S=7005838522 | en_HK |
dc.identifier.scopusauthorid | Scheller, J=7004433319 | en_HK |
dc.identifier.scopusauthorid | Watts, C=7202527327 | en_HK |
dc.identifier.issnl | 0021-9525 | - |