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Article: Ganoderma extract prevents albumin-induced oxidative damage and chemokines synthesis in cultured human proximal tubular epithelial cells
Title | Ganoderma extract prevents albumin-induced oxidative damage and chemokines synthesis in cultured human proximal tubular epithelial cells |
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Authors | |
Keywords | Chemokines Ganoderma Lingzhi Proteinuria Proximal tubular epithelial cells |
Issue Date | 2006 |
Publisher | Oxford University Press. The Journal's web site is located at http://ndt.oxfordjournals.org/ |
Citation | Nephrology Dialysis Transplantation, 2006, v. 21 n. 5, p. 1188-1197 How to Cite? |
Abstract | Background. Ganoderma lucidum (Ganoderma or lingzhi) is widely used as an alternative medicine remedy to promote health and longevity. Recent studies have indicated that components extracted from Ganoderma have a wide range of pharmacological actions including suppressing inflammation and scavenging free radicals. We recently reported that tubular secretion of interleukin-8 (IL-8) induced by albumin is important in the pathogenesis of tubulointerstitial injury in the proteinuric state. In this study, we explored the protective effect of Ganoderma extract (LZ) on albumin-induced kidney epithelial injury. Methods. Growth arrested human proximal tubular epithelial cells (PTECs) were incubated with 0.625 to 10 mg/ml human serum albumin (HSA) for up to 72 h. HSA induced DNA damage and apoptosis in PTEC in a dose- and time-dependent manner. Co-incubation of PTEC with 4 - 64 μg/ml LZ significantly reduced the oxidative damage and cytotoxic effect of HSA in a dose-dependent manner (P <0.001). Increased release of IL-8 and soluble intercellular adhesion molecules-1 (sICAM-1) in PTEC induced by HSA was ameliorated by co-incubation with Ganoderma (16 μg/ml). To explore the components of LZ that exhibited most protective effect in HSA-induced PTEC damages, LZ was further separated into two sub-fractions, LZF1 (MW <30 kDa) and LZF2 (MW <3 kDa), by molecular sieving using millipore membrane. PTEC were incubated with 5 mg/ml HSA in the presence of different doses of LZF1, LZF2 or unfractionated LZ. Results. There was no difference in the degree of protection from HSA-induced cytotoxicity or oxidative DNA damage between different fractions of LZ. However, low molecular weight LZ (<3 kDa) was most effective in reducing sICAM-1 released from HSA-activated PTEC whereas the high molecular weight LZ (unfractionated LZ) was more effective in diminishing IL-8 production. Conclusions. Our results suggest that Ganoderma significantly reduces oxidative damages and apoptosis in PTEC induced by HSA. The differential reduction of IL-8 or sICAM-1 released from HSA-activated PTEC by different components of the LZ implicates that components of Ganoderma with different molecular weights could play different roles and operate different mechanisms in preventing HSA-induced PTEC damage. © 2006 Oxford University Press. |
Persistent Identifier | http://hdl.handle.net/10722/78726 |
ISSN | 2023 Impact Factor: 4.8 2023 SCImago Journal Rankings: 1.414 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lai, KN | en_HK |
dc.contributor.author | Chan, LYY | en_HK |
dc.contributor.author | Tang, SCW | en_HK |
dc.contributor.author | Leung, JCK | en_HK |
dc.date.accessioned | 2010-09-06T07:46:03Z | - |
dc.date.available | 2010-09-06T07:46:03Z | - |
dc.date.issued | 2006 | en_HK |
dc.identifier.citation | Nephrology Dialysis Transplantation, 2006, v. 21 n. 5, p. 1188-1197 | en_HK |
dc.identifier.issn | 0931-0509 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/78726 | - |
dc.description.abstract | Background. Ganoderma lucidum (Ganoderma or lingzhi) is widely used as an alternative medicine remedy to promote health and longevity. Recent studies have indicated that components extracted from Ganoderma have a wide range of pharmacological actions including suppressing inflammation and scavenging free radicals. We recently reported that tubular secretion of interleukin-8 (IL-8) induced by albumin is important in the pathogenesis of tubulointerstitial injury in the proteinuric state. In this study, we explored the protective effect of Ganoderma extract (LZ) on albumin-induced kidney epithelial injury. Methods. Growth arrested human proximal tubular epithelial cells (PTECs) were incubated with 0.625 to 10 mg/ml human serum albumin (HSA) for up to 72 h. HSA induced DNA damage and apoptosis in PTEC in a dose- and time-dependent manner. Co-incubation of PTEC with 4 - 64 μg/ml LZ significantly reduced the oxidative damage and cytotoxic effect of HSA in a dose-dependent manner (P <0.001). Increased release of IL-8 and soluble intercellular adhesion molecules-1 (sICAM-1) in PTEC induced by HSA was ameliorated by co-incubation with Ganoderma (16 μg/ml). To explore the components of LZ that exhibited most protective effect in HSA-induced PTEC damages, LZ was further separated into two sub-fractions, LZF1 (MW <30 kDa) and LZF2 (MW <3 kDa), by molecular sieving using millipore membrane. PTEC were incubated with 5 mg/ml HSA in the presence of different doses of LZF1, LZF2 or unfractionated LZ. Results. There was no difference in the degree of protection from HSA-induced cytotoxicity or oxidative DNA damage between different fractions of LZ. However, low molecular weight LZ (<3 kDa) was most effective in reducing sICAM-1 released from HSA-activated PTEC whereas the high molecular weight LZ (unfractionated LZ) was more effective in diminishing IL-8 production. Conclusions. Our results suggest that Ganoderma significantly reduces oxidative damages and apoptosis in PTEC induced by HSA. The differential reduction of IL-8 or sICAM-1 released from HSA-activated PTEC by different components of the LZ implicates that components of Ganoderma with different molecular weights could play different roles and operate different mechanisms in preventing HSA-induced PTEC damage. © 2006 Oxford University Press. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Oxford University Press. The Journal's web site is located at http://ndt.oxfordjournals.org/ | en_HK |
dc.relation.ispartof | Nephrology Dialysis Transplantation | en_HK |
dc.rights | Nephrology, Dialysis, Transplantation. Copyright © Oxford University Press. | en_HK |
dc.subject | Chemokines | en_HK |
dc.subject | Ganoderma | en_HK |
dc.subject | Lingzhi | en_HK |
dc.subject | Proteinuria | en_HK |
dc.subject | Proximal tubular epithelial cells | en_HK |
dc.subject.mesh | Albumins | en_HK |
dc.subject.mesh | Apoptosis - drug effects | en_HK |
dc.subject.mesh | Cell Proliferation - drug effects | en_HK |
dc.subject.mesh | Cell Survival - drug effects | en_HK |
dc.subject.mesh | Cells, Cultured | en_HK |
dc.subject.mesh | Drugs, Chinese Herbal - pharmacology | en_HK |
dc.subject.mesh | Epithelial Cells - cytology - drug effects | en_HK |
dc.subject.mesh | Gene Expression Regulation | en_HK |
dc.subject.mesh | Humans | en_HK |
dc.subject.mesh | Intercellular Adhesion Molecule-1 - genetics - metabolism | en_HK |
dc.subject.mesh | Interleukin-8 - genetics - metabolism | en_HK |
dc.subject.mesh | Kidney Tubules, Proximal - cytology - drug effects | en_HK |
dc.subject.mesh | Oxidative Stress - drug effects - physiology | en_HK |
dc.subject.mesh | Reactive Oxygen Species - metabolism | en_HK |
dc.subject.mesh | Reference Values | en_HK |
dc.subject.mesh | Reverse Transcriptase Polymerase Chain Reaction | en_HK |
dc.subject.mesh | Sensitivity and Specificity | en_HK |
dc.title | Ganoderma extract prevents albumin-induced oxidative damage and chemokines synthesis in cultured human proximal tubular epithelial cells | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0931-0509&volume=21&issue=5&spage=1188&epage=1197&date=2006&atitle=Ganoderma+extract+prevents+albumin-induced+oxidative+damage+and+chemokines+synthesis+in+cultured+human+proximal+tubular+epithelial+cells | en_HK |
dc.identifier.email | Lai, KN: knlai@hku.hk | en_HK |
dc.identifier.email | Tang, SCW: scwtang@hku.hk | en_HK |
dc.identifier.email | Leung, JCK: jckleung@hku.hk | en_HK |
dc.identifier.authority | Lai, KN=rp00324 | en_HK |
dc.identifier.authority | Tang, SCW=rp00480 | en_HK |
dc.identifier.authority | Leung, JCK=rp00448 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1093/ndt/gfk085 | en_HK |
dc.identifier.pmid | 16434408 | - |
dc.identifier.scopus | eid_2-s2.0-33646201392 | en_HK |
dc.identifier.hkuros | 121476 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-33646201392&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 21 | en_HK |
dc.identifier.issue | 5 | en_HK |
dc.identifier.spage | 1188 | en_HK |
dc.identifier.epage | 1197 | en_HK |
dc.identifier.isi | WOS:000237004900010 | - |
dc.publisher.place | United Kingdom | en_HK |
dc.identifier.scopusauthorid | Lai, KN=7402135706 | en_HK |
dc.identifier.scopusauthorid | Chan, LYY=8108378300 | en_HK |
dc.identifier.scopusauthorid | Tang, SCW=7403437082 | en_HK |
dc.identifier.scopusauthorid | Leung, JCK=7202180349 | en_HK |
dc.identifier.citeulike | 599295 | - |
dc.identifier.issnl | 0931-0509 | - |