Structure and function study of Mp1p of pathogenic Talaromyces (Penicillium) marneffei: a novel virulence mechanism for the evasion of host defense

Dr Kao, Richard Yi Tsun   (Principal Investigator (PI))

Dr Sze Kong Hung   (Co-Investigator)

Professor Yuen Kwok Yung   (Co-Investigator)

36

2017-07-01

2017-06-30

1196004

Structure and function study of Mp1p of pathogenic Talaromyces (Penicillium) marneffei: a novel virulence mechanism for the evasion of host defense

NMR spectroscopy, protein structure, Talaromyces marneffei, Virulence factor Mp1p, virulence mechanism

Structural Biology,Microbiology

Biology and Medicine (M)

17124717

General Research Fund (GRF)

2017

Completed

1 Comparative metabolomics study on macrophage cells infected with WT TM vs Mp1p knockout strain 2 Use site-directed mutagenesis method to weaken the AA binding affinity of Mp1p-LBD2 to probe the effect of altering binding affinity and oligomeric state of Mp1p-LBD2. 3 Solve the solution structure of the apo-Mp1p-LBD2 by NMR methods and investigate whether it will be a monomeric or dimeric structure. 4 Backbone dynamics studies of Mp1p-LBD2 to compare the flexibility of apo-Mp1p-LBD2 and ligand bound Mp1p-LBD2. 5 Systematic structure and function characterizations of the Mp1p-LBD2 homologue:Mp1p-LBD1. 6 Determine the full-length Mp1p structure to investigate whether Mp1p-LBD1 and Mp1p-LBD2 interact with each other.