Development of DNA and viral vector vaccines against Epstein-Barr virus-positive nasopharyngeal carcinoma
Grant Data
Project Title
Development of DNA and viral vector vaccines against Epstein-Barr virus-positive nasopharyngeal carcinoma
Principal Investigator
Professor Lin, Chen-Lung
(Principal Investigator (PI))
Co-Investigator(s)
Dr Xu Xiaoning
(Co-Investigator)
Professor Austyn Jonathan M.
(Co-Investigator)
Duration
24
Start Date
2003-09-01
Amount
624000
Conference Title
Development of DNA and viral vector vaccines against Epstein-Barr virus-positive nasopharyngeal carcinoma
Presentation Title
Keywords
null
Discipline
Cancer
HKU Project Code
HKU 7444/03M
Grant Type
General Research Fund (GRF)
Funding Year
2003
Status
Completed
Objectives
To design and parpare DNA vaccine encoding EBNA1 and LMP2, and the MVA vaccine encoding the cytokine GM-CSF, the chemokine RANTES, the EBV- specific lytic antigen EBNA-1, and EBV-specific latent antigen LMP2; to identify the efficacy of these vaccines in vitro on their capacity in attracting dendritic cells and also in the induction of EBNA-1 specific CD4+T cell response and LMP2-specific CTL immunity; to verify the efficacy of these vaccines in a mouse NPC and a B cell lymphoma model, including the potency of CD4+ and CTL immunity, and also to test the effect of the dosage, route, and the effect of combinational use of both the DNA and MVA vaccines.