Rapid Long-Read Profiling of Genomic Structural Variants To Improve The Clinical Stratification of ALL
Grant Data
Project Title
Rapid Long-Read Profiling of Genomic Structural Variants To Improve The Clinical Stratification of ALL
Principal Investigator
Professor Wong, Wing Hon Jason
(Principal Investigator (PI))
Duration
24
Start Date
2022-12-01
Amount
1058368
Conference Title
Rapid Long-Read Profiling of Genomic Structural Variants To Improve The Clinical Stratification of ALL
Keywords
Rapid Long-Read Profiling , Genomic Structural Variants , Improve The Clinical Stratification
Discipline
Biology and Medicine
HKU Project Code
MHP/054/21
Grant Type
Mainland-Hong Kong Joint Funding Scheme (MHKJFS)
Funding Year
2022
Status
On-going
Objectives
The purpose of this project is to develop a targeted long read sequencing based platform for the rapid detection of structural variants (SVs) in cancer genomes using Cas9-targeted technology.The project will involve the development of:(1) A scalable bioinformatics platform for SV detection.(2) Perform long read sequencing whole genome sequencing and higher-order chromatin interactions to identify functional SVs in acute lymphoblastic leukaemia (ALL).(3) Develop PCR-free CRISPR/Cas9 based targeted sequencing panel for rapid SV detection.The methods involved will include bioinformatics analysis, nanopore long read sequencing and Cas9-based targeted excision of DNA for sequencing.This project will enable detection of genomic variants that cannot be accurately detected by existing methods. It will fill the gap in the field of cancer precision medicine, and especially benefiting ALL patients.