Application of real-time fluorescent biosensors in living plants
Grant Data
Project Title
Application of real-time fluorescent biosensors in living plants
Principal Investigator
Dr Lim, Shey Li
(Principal Investigator (PI))
Duration
36
Start Date
2023-05-01
Amount
13130
Conference Title
Application of real-time fluorescent biosensors in living plants
Keywords
Application, real-time, fluorescent, biosensors, plants
Discipline
Plant Sciences/ Plant BiologyBiological Imaging
Panel
Biology and Medicine (M)
HKU Project Code
PDFS2324-7S01
Grant Type
RGC Postdoctoral Fellowship Scheme 2023/24
Funding Year
2023
Status
On-going
Objectives
To develop second-generation NADPH and NADH/NAD+ biosensors (mCherry-iNAPs/SoNar) for in planta application.To introduce the novel pyruvate biosensors (PyronicSF and Dead-PyronicSF) to Arabidopsis for in planta application.To prepare plant cell atlases to visualize the ATP, NADPH, NADH/NAD+ ratio, and pyruvate pools in different subcellular compartments and plant organs and examine their dynamic changes under various experimental conditions4. To study photosynthesis of the Arabidopsis bundle sheath cells and compare with that of mesophyll cells using the novel sensors developed in this project.