Tracking, visualizing and characterization of normal and ""auto-reactive"" T and B cells in steady state and in ""danger""

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Grant Data
Project Title
Tracking, visualizing and characterization of normal and ""auto-reactive"" T and B cells in steady state and in ""danger""
Principal Investigator
Dr Huang, Fang-Ping   (Principal Investigator (PI))
Co-Investigator(s)
Dr MacPherson George Gordon   (Co-Investigator)
Duration
12
Start Date
2001-02-01
Amount
120000
Conference Title
Tracking, visualizing and characterization of normal and ""auto-reactive"" T and B cells in steady state and in ""danger""
Presentation Title
Keywords
null
Discipline
N/A
HKU Project Code
N/A
Grant Type
Research Initiation Programme
Funding Year
2000
Status
Completed
Objectives
To study mechanisms of peripheral tolerance by tracking adoptively transferred labeledT and B cells in semi-allogeneic and syngeneic rat models; to determine the effects of inflammatory stimuli on antigen presenting cells and immune effector T and B lymphocytes both in normal and in ""auto-reactive"" situations; to compare dendritic cell (DC) functions and allogeneic T and B cell responses between conventional and specific pathogen free (SPF) animals; to compare cytokine expression profiles (IL-10, IL-12, IFN-[gamma], TGF-[beta] etc.) of proliferating and non-proliferating CD8+ and CD4+ T cells, and of host APC, after co-injection of bacterial product (LPS) into the animals; to characterize and to compare cells with T regulatory (Treg) phenotypes generated in the semi-allogeneic and syngeneic models after cell transfer under steady state and in the presence of inflamatory stimuli.