Comparing differentiation propensity among mescenchymal stem cells derived from sources of human embryonic stem cell lines, term placenta and bone morrow

Dr Lian, Qizhou   (Principal Investigator (PI))

Professor Tse Hung Fat   (Co-Investigator)

Dr Ling Lieng Hsi   (Co-Investigator)

24

2008-10-16

2010-10-15

120000

Comparing differentiation propensity among mescenchymal stem cells derived from sources of human embryonic stem cell lines, term placenta and bone morrow

bone marrow, Differentiation propensity, heart protection, human embryonic stem cells, mescenchymal stem cells, term placenta

Cell Biology,Cardiovascular Research

200809159006

Seed Fund for PI Research – Basic Research

2008

Completed

Human mescenchymal stem cells (MSCs) have been emerged as very valuable cell sources in regenerative medicine. Different tissue-origin derived MSCs share many common properties including conventional capacity of cartilage, fat and bone differentiation. However, there are many biologically differences including multilineage differentiation potential. The different tissue origin-derived MSC with distinct differentiation propensity is one of the most interesting issues and is largely unclear at present. To eventually provide the best MSC source for tissue regeneration in clinical applications and research purpose, therefore the central objective of this proposal is to systematically compare propensity of multilineage differentiation among MSCs derived from sources of human embryonic stem cells (from early embryonic-like stage ,hESC-MSCs), term placenta (from neonatal stage ,PL-MSCs) and adult bone marrow (from adult stage ,BM-MSCs). The specific aims to achieve central goal are to 1. Identifying molecular bases for specific lineage or cell type differentiation potential among hESC-MSCs, PL-MSCs and BM-MSCs by fluorescence-activated cell sorting (FACS), microarray and quantitative PCR analysis. 2. Determining propensity of multilineage differentiation (mesoderm, ectoderm and endoderm differentiation) among hESC-MSCs, PL-MSCs and BM-MSCs by in vitro differentiation studies. 3. Verifying functional characteristics of differentially differentiation potential among hESC-MSCs, PL-MSCs and BM-MSCs by in vivo transplantation studies. These studies will facilitate our understanding of biological characteristics among MSCs derived from different tissue-origins for the development of stem cell-based therapies.