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Conference Paper: Sulindac metabolites induces XAF1 expression byinhibition of extracellular signal-regulated kinase 1/2phosphorylation in human gastric cancer cells

TitleSulindac metabolites induces XAF1 expression byinhibition of extracellular signal-regulated kinase 1/2phosphorylation in human gastric cancer cells
Authors
Issue Date2005
PublisherBlackwell Publishing Asia.
Citation
Asian Pacific Digestive Week, Seoul, Korea, 25-28 September 2005. In Journal of Gastroenterology and Hepatology, 2005, v. 20 n. S2, p. A298 Abstract no. WO018 How to Cite?
AbstractBackground/Aims Sulindac metabolites can inhibit the phospho-rylation of extracellular signal-regulated kinase (ERK) 1/2 and induceapoptosis with mechanisms not fully understood. Recently, XIAP-associated factor 1 (XAF1) has been identified to negatively regulatethe function of X-linked inhibitor of apoptosis (XIAP). In this study,we investigated the role of XAF1 in gastric cancer cell lines inducedby ERK1/2 suppression and treatment with sulindac metabolites.Methods Three human gastric cancer cell lines were studied,including AGS (p53 wide type), BCG823 (p53 mutant) and Kato III(p53 deficiency). The effect of U0126 (a MEK-specific inhibitor) onMEK/ERK signaling pathway and XAF1 expression were detected byWestern blotting, so did sulindac metabolites. The growth rate ofBCG823 and its XAF1 stable transfectants were evaluated by MTTassay with or without treatment of two sulindac metabolites, sulfideand sulfone, respectively.Results The original expressions of XAF1 proteins were very lowin AGS and BCG823 cells. But the levels were increased after treat-ment with U0126 (30 uM) for 24 h without down-regulation of XIAP.The expression of XAF1 had no alteration in Kato III cells with orwithout ERK inhibitor. Phospho-p44/42 and p-MEK1/2 proteinlevels were markedly decreased by treatment of U0126 in these threegastric cancer cells. XAF1 expression could also be induced by sulin-dac metabolites, sulfide and sulfone. Both sulfide and sulfone sup-pressed the growth of BCG823 cells. Stable transfection with XAF1rendered BCG823 cells more susceptible to sulindac metabolites-induced apoptosis in doses (Sulfide 60–160 uM, Sulfone 200–600 uM) and time (1–3 days) dependent manner.Conclusions These findings suggested that inactivation of ERK1/2could induce XAF1 expression which may correlate with the differ-ent type of p53 in gastric cancer cells. Over-expression of XAF1 mayimprove the sensitivity of gastric cancer cells to sulindac metabolites.
Persistent Identifierhttp://hdl.handle.net/10722/102236
ISSN
2023 Impact Factor: 3.7
2023 SCImago Journal Rankings: 1.179

 

DC FieldValueLanguage
dc.contributor.authorYu, Len_HK
dc.contributor.authorWang, Jen_HK
dc.contributor.authorZou, Ben_HK
dc.contributor.authorLin, MCen_HK
dc.contributor.authorWu, YLen_HK
dc.contributor.authorWong, BCYen_HK
dc.date.accessioned2010-09-25T20:22:31Z-
dc.date.available2010-09-25T20:22:31Z-
dc.date.issued2005en_HK
dc.identifier.citationAsian Pacific Digestive Week, Seoul, Korea, 25-28 September 2005. In Journal of Gastroenterology and Hepatology, 2005, v. 20 n. S2, p. A298 Abstract no. WO018en_HK
dc.identifier.issn0815-9319en_HK
dc.identifier.urihttp://hdl.handle.net/10722/102236-
dc.description.abstractBackground/Aims Sulindac metabolites can inhibit the phospho-rylation of extracellular signal-regulated kinase (ERK) 1/2 and induceapoptosis with mechanisms not fully understood. Recently, XIAP-associated factor 1 (XAF1) has been identified to negatively regulatethe function of X-linked inhibitor of apoptosis (XIAP). In this study,we investigated the role of XAF1 in gastric cancer cell lines inducedby ERK1/2 suppression and treatment with sulindac metabolites.Methods Three human gastric cancer cell lines were studied,including AGS (p53 wide type), BCG823 (p53 mutant) and Kato III(p53 deficiency). The effect of U0126 (a MEK-specific inhibitor) onMEK/ERK signaling pathway and XAF1 expression were detected byWestern blotting, so did sulindac metabolites. The growth rate ofBCG823 and its XAF1 stable transfectants were evaluated by MTTassay with or without treatment of two sulindac metabolites, sulfideand sulfone, respectively.Results The original expressions of XAF1 proteins were very lowin AGS and BCG823 cells. But the levels were increased after treat-ment with U0126 (30 uM) for 24 h without down-regulation of XIAP.The expression of XAF1 had no alteration in Kato III cells with orwithout ERK inhibitor. Phospho-p44/42 and p-MEK1/2 proteinlevels were markedly decreased by treatment of U0126 in these threegastric cancer cells. XAF1 expression could also be induced by sulin-dac metabolites, sulfide and sulfone. Both sulfide and sulfone sup-pressed the growth of BCG823 cells. Stable transfection with XAF1rendered BCG823 cells more susceptible to sulindac metabolites-induced apoptosis in doses (Sulfide 60–160 uM, Sulfone 200–600 uM) and time (1–3 days) dependent manner.Conclusions These findings suggested that inactivation of ERK1/2could induce XAF1 expression which may correlate with the differ-ent type of p53 in gastric cancer cells. Over-expression of XAF1 mayimprove the sensitivity of gastric cancer cells to sulindac metabolites.-
dc.languageengen_HK
dc.publisherBlackwell Publishing Asia.en_HK
dc.relation.ispartofJournal of Gastroenterology and Hepatologyen_HK
dc.titleSulindac metabolites induces XAF1 expression byinhibition of extracellular signal-regulated kinase 1/2phosphorylation in human gastric cancer cellsen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0815-9319&volume=20&spage=Supp A299&epage=&date=2005&atitle=Sulindac+Metabolites+Induces+XAF1+Expression+by+Inhibition+of+Extracellular+Signal-regulated+Kinase+1/2+Phosphorylation+in+Human+Gastric+Cancer+Cells.+ Oral+presentation,+Asian+Pacific+Digestive+Week+2005,+Sept.+25-28,+2005,+Seoul,+Koreaen_HK
dc.identifier.emailYu, L: graceyu1028@sohu.comen_HK
dc.identifier.emailWang, J: jidewang@gmail.comen_HK
dc.identifier.emailZou, B: zoubing@hkucc.hku.hken_HK
dc.identifier.emailLin, MC: mcllin@HKUCC.hku.hken_HK
dc.identifier.emailWong, BCY: bcywong@hku.hken_HK
dc.identifier.authorityWang, J=rp00491en_HK
dc.identifier.authorityLin, MC=rp00746en_HK
dc.identifier.authorityWong, BCY=rp00429en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1440-1746.2005.04101.x-
dc.identifier.hkuros117562en_HK
dc.identifier.volume20en_HK
dc.identifier.issnl0815-9319-

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