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Conference Paper: Effects of Docosahexaenoic Acid and Eicosapentaenoic Acid on the Differential Protein Expression in Human Hepatocellular Carcinoma Cells

TitleEffects of Docosahexaenoic Acid and Eicosapentaenoic Acid on the Differential Protein Expression in Human Hepatocellular Carcinoma Cells
Authors
Issue Date2007
PublisherInternational Institute of Anticancer Research. The Journal's web site is located at http://cgp.iiarjournals.org/
Citation
The 2nd International Conference of the Hellenic Proteomics Society, Crete, Greece, 23-25 May 2007. Abstract in Cancer Genomics & Proteomics, 2007, v. 4 n. 4, p. 279-280 How to Cite?
AbstractHepatocellular carcinoma (HCC) plays a leading role in causing cancer death due to its high metastatic potential and frequent tumor recurrence rate after treatment. Docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) are two important omega 3-polyunsaturated fatty acids (ˆ3-PUFAs) which have been shown to be able to inhibit the growth of hepatocarcinoma in animal models; however, the mechanisms are not fully understood. Adopting a proteomic approach, we investigated the effects of DHA and EPA on the expression of proteins in the human hepatocellular carcinoma cell line PLC with no metastatic potential. Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) was employed for protein separation in the subcellular fractions prior to fluorescent protein staining by SyproRuby protein gel stain. Comparison of differential protein expression between control and fatty acid treatments and between DHA and EPA treatments was enabled by image analysis and protein spots were identified by mass spectrometry (MALDI-TOF/TOF). Results indicate that DHA (200 ÌM) was more effective at causing PLC cell death than EPA (200 ÌM). DHA down-regulated the expression of annexin A2, a protein which is closely associated with angiogenesis and metastasis, and placental anticoagulant protein II (PP4-X), which is involved in the morphological diversification and dissemination of human carcinoma. The N-myc downstream regulated gene 1, a metastasis suppressor gene, is up-regulated in EPA-treated cancer cells whereas the ubiquinol-cytochrome c reductase core protein I (UQCRC1) is down-regulated. UQCRC1 is involved in mitochondria-to-nucleus retrograde response in human cancer and is highly expressed in breast and ovarian tumors. These data not only facilitate the identification of differentially expressed proteins that are involved in hepatocellular carcinogenesis, but may also provide candidate biomarkers for the development of therapeutic tools with dietary fatty acids.
Persistent Identifierhttp://hdl.handle.net/10722/110347
ISSN
2023 Impact Factor: 2.6
2023 SCImago Journal Rankings: 0.722

 

DC FieldValueLanguage
dc.contributor.authorJor, IWYen_HK
dc.contributor.authorLee, CYKen_HK
dc.contributor.authorSit, WHen_HK
dc.contributor.authorWong, Len_HK
dc.contributor.authorFan, STen_HK
dc.contributor.authorKwan, Men_HK
dc.contributor.authorWan, JMFen_HK
dc.date.accessioned2010-09-26T02:02:01Z-
dc.date.available2010-09-26T02:02:01Z-
dc.date.issued2007en_HK
dc.identifier.citationThe 2nd International Conference of the Hellenic Proteomics Society, Crete, Greece, 23-25 May 2007. Abstract in Cancer Genomics & Proteomics, 2007, v. 4 n. 4, p. 279-280en_HK
dc.identifier.issn1109-6535-
dc.identifier.urihttp://hdl.handle.net/10722/110347-
dc.description.abstractHepatocellular carcinoma (HCC) plays a leading role in causing cancer death due to its high metastatic potential and frequent tumor recurrence rate after treatment. Docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) are two important omega 3-polyunsaturated fatty acids (ˆ3-PUFAs) which have been shown to be able to inhibit the growth of hepatocarcinoma in animal models; however, the mechanisms are not fully understood. Adopting a proteomic approach, we investigated the effects of DHA and EPA on the expression of proteins in the human hepatocellular carcinoma cell line PLC with no metastatic potential. Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) was employed for protein separation in the subcellular fractions prior to fluorescent protein staining by SyproRuby protein gel stain. Comparison of differential protein expression between control and fatty acid treatments and between DHA and EPA treatments was enabled by image analysis and protein spots were identified by mass spectrometry (MALDI-TOF/TOF). Results indicate that DHA (200 ÌM) was more effective at causing PLC cell death than EPA (200 ÌM). DHA down-regulated the expression of annexin A2, a protein which is closely associated with angiogenesis and metastasis, and placental anticoagulant protein II (PP4-X), which is involved in the morphological diversification and dissemination of human carcinoma. The N-myc downstream regulated gene 1, a metastasis suppressor gene, is up-regulated in EPA-treated cancer cells whereas the ubiquinol-cytochrome c reductase core protein I (UQCRC1) is down-regulated. UQCRC1 is involved in mitochondria-to-nucleus retrograde response in human cancer and is highly expressed in breast and ovarian tumors. These data not only facilitate the identification of differentially expressed proteins that are involved in hepatocellular carcinogenesis, but may also provide candidate biomarkers for the development of therapeutic tools with dietary fatty acids.-
dc.languageengen_HK
dc.publisherInternational Institute of Anticancer Research. The Journal's web site is located at http://cgp.iiarjournals.org/-
dc.relation.ispartofCancer Genomics & Proteomicsen_HK
dc.titleEffects of Docosahexaenoic Acid and Eicosapentaenoic Acid on the Differential Protein Expression in Human Hepatocellular Carcinoma Cellsen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailSit, WH: whsit@HKUCC.hku.hken_HK
dc.identifier.emailWan, JMF: jmfwan@hkusua.hku.hken_HK
dc.identifier.authorityWan, JMF=rp00798en_HK
dc.identifier.hkuros129400en_HK
dc.identifier.volume4en_HK
dc.identifier.issue4en_HK
dc.identifier.spage279en_HK
dc.identifier.epage280-
dc.publisher.placeGreece-
dc.identifier.issnl1109-6535-

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