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Conference Paper: Cyclooxygenase-2 is an essential mediator of gonadotropins-induced apoptosis of human ovarian surface epithelial cells
Title | Cyclooxygenase-2 is an essential mediator of gonadotropins-induced apoptosis of human ovarian surface epithelial cells |
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Authors | |
Issue Date | 2006 |
Publisher | Hong Kong Academy of Medicine |
Citation | The 4th International Huaxia Congress of Endocrinology, Hong Kong, 15–18 December 2006. In Hong Kong Medical Journal, 2006, v. 12 n. 6 S4, p. 69 Abstract no. O53 How to Cite? |
Abstract | Objective: To investigate the signaling mechanism underlying gonadotropins-mediated apoptosis of human ovarian
surface epithelial (OSE) cells. Methods: OSE cells were treated with surge concentrations of gonadotropins (FSH
and LH/hCG), membrane, cytosolic, and nuclear fractions were prepared by differential centrifugation. Expression of
N-cadherin, b-catenin, and cyclooxygnease-2 (COX-2) were examined by immunoblot analysis. b-catenin/T-cell factor
(TCF) transcriptional activity was measured in a luiferase reporter gene assay. Results: We demonstrated that disruption
of N-cadherin-mediated cell-cell adhesion by gonadotropins is an important molecular event in the apoptosis in human
OSE. This downregulation of N-cadherin was correlated with a redistribution of b-catenin from the plasma membrane to
the nucleus, resulting in the activation of b-catenin/T Cell Factor (TCF) transcription. These events were mediated via a
phosphatidylinositol 3-kinase (PI3K)-dependent phosphorylation of the inhibitory serine 9 residue of glycogen synthase
kinase-3b (GSK3-b). In addition, we showed that COX-2, a target of b-catenin/TCF, is an important mediator of the
gonadotropins-induced apoptosis of OSE. Conclusion: These findings shed new light on the molecular mechanism
underlying OSE survival and death, a process that is anticipated to have important implication in the ovulation and
ovarian cancer risk. |
Persistent Identifier | http://hdl.handle.net/10722/110452 |
ISSN | 2023 Impact Factor: 3.1 2023 SCImago Journal Rankings: 0.261 |
DC Field | Value | Language |
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dc.contributor.author | Pon, YL | en_HK |
dc.contributor.author | Wong, AST | en_HK |
dc.date.accessioned | 2010-09-26T02:06:32Z | - |
dc.date.available | 2010-09-26T02:06:32Z | - |
dc.date.issued | 2006 | en_HK |
dc.identifier.citation | The 4th International Huaxia Congress of Endocrinology, Hong Kong, 15–18 December 2006. In Hong Kong Medical Journal, 2006, v. 12 n. 6 S4, p. 69 Abstract no. O53 | - |
dc.identifier.issn | 1024-2708 | - |
dc.identifier.uri | http://hdl.handle.net/10722/110452 | - |
dc.description.abstract | Objective: To investigate the signaling mechanism underlying gonadotropins-mediated apoptosis of human ovarian surface epithelial (OSE) cells. Methods: OSE cells were treated with surge concentrations of gonadotropins (FSH and LH/hCG), membrane, cytosolic, and nuclear fractions were prepared by differential centrifugation. Expression of N-cadherin, b-catenin, and cyclooxygnease-2 (COX-2) were examined by immunoblot analysis. b-catenin/T-cell factor (TCF) transcriptional activity was measured in a luiferase reporter gene assay. Results: We demonstrated that disruption of N-cadherin-mediated cell-cell adhesion by gonadotropins is an important molecular event in the apoptosis in human OSE. This downregulation of N-cadherin was correlated with a redistribution of b-catenin from the plasma membrane to the nucleus, resulting in the activation of b-catenin/T Cell Factor (TCF) transcription. These events were mediated via a phosphatidylinositol 3-kinase (PI3K)-dependent phosphorylation of the inhibitory serine 9 residue of glycogen synthase kinase-3b (GSK3-b). In addition, we showed that COX-2, a target of b-catenin/TCF, is an important mediator of the gonadotropins-induced apoptosis of OSE. Conclusion: These findings shed new light on the molecular mechanism underlying OSE survival and death, a process that is anticipated to have important implication in the ovulation and ovarian cancer risk. | - |
dc.language | eng | en_HK |
dc.publisher | Hong Kong Academy of Medicine | - |
dc.relation.ispartof | Hong Kong Medical Journal | en_HK |
dc.title | Cyclooxygenase-2 is an essential mediator of gonadotropins-induced apoptosis of human ovarian surface epithelial cells | en_HK |
dc.type | Conference_Paper | en_HK |
dc.identifier.email | Wong, AST: awong1@hkucc.hku.hk | en_HK |
dc.identifier.authority | Wong, AST=rp00805 | en_HK |
dc.identifier.hkuros | 130206 | en_HK |
dc.identifier.issnl | 1024-2708 | - |