File Download
There are no files associated with this item.
Supplementary
-
Citations:
- Appears in Collections:
Conference Paper: C-Type Natriuretic peptide (CNP) release does not contribute to endothelium-derived hyperpolarizing factor (EDHF) mediated relaxation in porcine coronary artery
Title | C-Type Natriuretic peptide (CNP) release does not contribute to endothelium-derived hyperpolarizing factor (EDHF) mediated relaxation in porcine coronary artery |
---|---|
Authors | |
Issue Date | 2008 |
Publisher | Federation of American Societies for Experimental Biology. Meeting abstracts can be accessed via http://www.fasebj.org/search.dtl |
Citation | The 2008 Annual Meeting of Experimental Biology (EB 2008) - ASPET's Centennial Meeting, San Diego, CA., 5-9 April 2008. In The FASEB Journal, 2008, v. 22 meeting abstract suppl., no. 914.3 How to Cite? |
Abstract | Objective: The release of CNP accounts for EDHF-mediated relaxations in the rat mesenteric artery1 and is Gi protein dependent2. EDHF-mediated relaxations in the porcine coronary artery are not Gi dependent3. This study investigates whether or not CNP release accounts for EDHF-responses in this artery.
Methods: (1) CNP concentration was measured by enzyme immunoassay in bathing solution containing porcine coronary arterial rings where EDHF response was initiated by bradykinin (100 nM). (2) EDHF-mediated relaxations induced by SFLLRN were compared with those to exogenous CNP in the presence of phosphoramidon (10 µM; known to potentiate CNP induced relaxations).
Results: There was no significant difference in the CNP concentrations between chambers containing rings that did or did not exhibit a EDHF-mediated response. Relaxations induced by CNP alone were potentiated by phosphoramidon (maximal relaxation 60.7 ± 7.7 % vs 84.1 ± 7.5 %, control vs phosphoramidon, P<0.05) but the EDHF-mediated responses to SFLLRN were not (maximal relaxation 69.9 ± 9.1 % vs 72.8 ± 9.9 %, control vs phosphoramidon)
Conclusions: Unlike in the mesenteric artery and the heart of the rat, CNP release does not contribute to EDHF-mediated relaxations in the porcine coronary artery.
FOOTNOTES
Support: HKU CRCG (No. 21374077)
REFERENCES
Villar , et al. Cardiovasc Res 2007914.3
Chauhan , et al. PNAS 2003914.3
Ng , et al. J Vasc Res 200512 S2010 |
Persistent Identifier | http://hdl.handle.net/10722/112777 |
ISSN | 2023 Impact Factor: 4.4 2023 SCImago Journal Rankings: 1.412 |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ng, JKF | en_HK |
dc.contributor.author | Leung, SWS | en_HK |
dc.contributor.author | Man, RYK | en_HK |
dc.contributor.author | Vanhoutte, PMGR | en_HK |
dc.date.accessioned | 2010-09-26T03:46:45Z | - |
dc.date.available | 2010-09-26T03:46:45Z | - |
dc.date.issued | 2008 | en_HK |
dc.identifier.citation | The 2008 Annual Meeting of Experimental Biology (EB 2008) - ASPET's Centennial Meeting, San Diego, CA., 5-9 April 2008. In The FASEB Journal, 2008, v. 22 meeting abstract suppl., no. 914.3 | - |
dc.identifier.issn | 0892-6638 | - |
dc.identifier.uri | http://hdl.handle.net/10722/112777 | - |
dc.description.abstract | Objective: The release of CNP accounts for EDHF-mediated relaxations in the rat mesenteric artery1 and is Gi protein dependent2. EDHF-mediated relaxations in the porcine coronary artery are not Gi dependent3. This study investigates whether or not CNP release accounts for EDHF-responses in this artery. Methods: (1) CNP concentration was measured by enzyme immunoassay in bathing solution containing porcine coronary arterial rings where EDHF response was initiated by bradykinin (100 nM). (2) EDHF-mediated relaxations induced by SFLLRN were compared with those to exogenous CNP in the presence of phosphoramidon (10 µM; known to potentiate CNP induced relaxations). Results: There was no significant difference in the CNP concentrations between chambers containing rings that did or did not exhibit a EDHF-mediated response. Relaxations induced by CNP alone were potentiated by phosphoramidon (maximal relaxation 60.7 ± 7.7 % vs 84.1 ± 7.5 %, control vs phosphoramidon, P<0.05) but the EDHF-mediated responses to SFLLRN were not (maximal relaxation 69.9 ± 9.1 % vs 72.8 ± 9.9 %, control vs phosphoramidon) Conclusions: Unlike in the mesenteric artery and the heart of the rat, CNP release does not contribute to EDHF-mediated relaxations in the porcine coronary artery. FOOTNOTES Support: HKU CRCG (No. 21374077) REFERENCES Villar , et al. Cardiovasc Res 2007914.3 Chauhan , et al. PNAS 2003914.3 Ng , et al. J Vasc Res 200512 S2010 | - |
dc.language | eng | en_HK |
dc.publisher | Federation of American Societies for Experimental Biology. Meeting abstracts can be accessed via http://www.fasebj.org/search.dtl | en_HK |
dc.relation.ispartof | The FASEB Journal | en_HK |
dc.title | C-Type Natriuretic peptide (CNP) release does not contribute to endothelium-derived hyperpolarizing factor (EDHF) mediated relaxation in porcine coronary artery | en_HK |
dc.type | Conference_Paper | en_HK |
dc.identifier.email | Ng, JKF: jkfng@hkucc.hku.hk | en_HK |
dc.identifier.email | Leung, SWS: swsleung@HKUCC.hku.hk | en_HK |
dc.identifier.email | Man, RYK: rykman@hkucc.hku.hk | en_HK |
dc.identifier.email | Vanhoutte, PMGR: vanhoutt@hku.hk | en_HK |
dc.identifier.authority | Ng, JKF=rp00544 | en_HK |
dc.identifier.authority | Leung, SWS=rp00235 | en_HK |
dc.identifier.authority | Man, RYK=rp00236 | en_HK |
dc.identifier.hkuros | 168524 | en_HK |
dc.identifier.hkuros | 147371 | - |
dc.identifier.volume | 22 | - |
dc.identifier.issue | meeting abstract suppl. | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0892-6638 | - |