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Article: Stem-like cancer cells are inducible by increasing genomic instability in cancer cells
Title | Stem-like cancer cells are inducible by increasing genomic instability in cancer cells | ||||||||
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Authors | |||||||||
Issue Date | 2010 | ||||||||
Publisher | American Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/ | ||||||||
Citation | Journal Of Biological Chemistry, 2010, v. 285 n. 7, p. 4931-4940 How to Cite? | ||||||||
Abstract | The existence of cancer stem cells (CSCs) or stem-like cancer cells (SLCCs) is regarded as the cause of tumor formation and recurrence. However, the origin of such cells remains controversial with two competing hypotheses:CSCsare either transformed from tissue adult stem cells or dedifferentiated from transformed progenitor cells. Compelling evidence has determined the chromosomal aneuploidy to be one of the hallmarks of cancer cells, indicating genome instability plays an important role in tumorigenesis, for which CSCs are believed to be the initiator. To gain direct evidence that genomic instability is involved in the induction of SLCCs, we utilized multiple approaches to enhance genomic instability and monitored the percentage of SLCC in cultured cancer cells. Using side population (SP) cells as a marker for SLCC in human nasopharyngeal carcinoma (NPC) and CD133 for human neuroblastoma cells,wefound thatDNAdamageinducers,UVand mitomycin C were capable of increasing SP cells in NPCCNE-2 and neuroblastoma SKN-SH cells. Likewise, either overexpression of a key regulator of cell cycle, Mad2, or knock down of Aurora B, an important kinase in mitosis, or Cdh1, a key E3 ligase in cell cycle, resulted in a significant increase of SP cells in CNE-2. More interestingly, enrichment of SP cells was observed in recurrent tumor tissues as compared with the primary tumor in the same NPC patients. Our study thus suggested that, beside transformation of tissue stem cells leading to CSC generation, genomic instability could be another potential mechanism resulting in SLCC formation, especially at tumor recurrence stage. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc. | ||||||||
Persistent Identifier | http://hdl.handle.net/10722/124474 | ||||||||
ISSN | 2020 Impact Factor: 5.157 2023 SCImago Journal Rankings: 1.766 | ||||||||
PubMed Central ID | |||||||||
ISI Accession Number ID |
Funding Information: The work was supported by the Chinese National 973 Program (Grants 2006CB910104 and 2010CB912201), the Chinese National 863 Program (Grant 2006AA02A404), and the Guangdong Province-National Natural Science Foundation of China Cooperation Program (Grant u0732005). | ||||||||
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Liang, Y | en_HK |
dc.contributor.author | Zhong, Z | en_HK |
dc.contributor.author | Huang, Y | en_HK |
dc.contributor.author | Deng, W | en_HK |
dc.contributor.author | Cao, J | en_HK |
dc.contributor.author | Tsao, G | en_HK |
dc.contributor.author | Liu, Q | en_HK |
dc.contributor.author | Pei, D | en_HK |
dc.contributor.author | Kang, T | en_HK |
dc.contributor.author | Zeng, YX | en_HK |
dc.date.accessioned | 2010-10-31T10:36:26Z | - |
dc.date.available | 2010-10-31T10:36:26Z | - |
dc.date.issued | 2010 | en_HK |
dc.identifier.citation | Journal Of Biological Chemistry, 2010, v. 285 n. 7, p. 4931-4940 | en_HK |
dc.identifier.issn | 0021-9258 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/124474 | - |
dc.description.abstract | The existence of cancer stem cells (CSCs) or stem-like cancer cells (SLCCs) is regarded as the cause of tumor formation and recurrence. However, the origin of such cells remains controversial with two competing hypotheses:CSCsare either transformed from tissue adult stem cells or dedifferentiated from transformed progenitor cells. Compelling evidence has determined the chromosomal aneuploidy to be one of the hallmarks of cancer cells, indicating genome instability plays an important role in tumorigenesis, for which CSCs are believed to be the initiator. To gain direct evidence that genomic instability is involved in the induction of SLCCs, we utilized multiple approaches to enhance genomic instability and monitored the percentage of SLCC in cultured cancer cells. Using side population (SP) cells as a marker for SLCC in human nasopharyngeal carcinoma (NPC) and CD133 for human neuroblastoma cells,wefound thatDNAdamageinducers,UVand mitomycin C were capable of increasing SP cells in NPCCNE-2 and neuroblastoma SKN-SH cells. Likewise, either overexpression of a key regulator of cell cycle, Mad2, or knock down of Aurora B, an important kinase in mitosis, or Cdh1, a key E3 ligase in cell cycle, resulted in a significant increase of SP cells in CNE-2. More interestingly, enrichment of SP cells was observed in recurrent tumor tissues as compared with the primary tumor in the same NPC patients. Our study thus suggested that, beside transformation of tissue stem cells leading to CSC generation, genomic instability could be another potential mechanism resulting in SLCC formation, especially at tumor recurrence stage. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc. | en_HK |
dc.language | eng | en_HK |
dc.publisher | American Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/ | en_HK |
dc.relation.ispartof | Journal of Biological Chemistry | en_HK |
dc.rights | Journal of Biological Chemistry. Copyright © American Society for Biochemistry and Molecular Biology, Inc. | - |
dc.rights | This research was originally published in [Journal Name]. Author(s). Title. Journal Name. Year. Vol:pp-pp. © the American Society for Biochemistry and Molecular Biology | - |
dc.subject.mesh | ATP-Binding Cassette Transporters - metabolism | - |
dc.subject.mesh | Antigens, CD - metabolism | - |
dc.subject.mesh | Blotting, Western | - |
dc.subject.mesh | Genomic Instability - genetics - physiology | - |
dc.subject.mesh | Neoplastic Stem Cells - cytology - metabolism | - |
dc.title | Stem-like cancer cells are inducible by increasing genomic instability in cancer cells | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9258&volume=285&issue=7&spage=4931&epage=4940&date=2010&atitle=Stem-like+cancer+cells+are+inducible+by+increasing+genomic+instability+in+cancer+cells | - |
dc.identifier.email | Deng, W: wdeng@hkucc.hku.hk | en_HK |
dc.identifier.email | Tsao, G: gswtsao@hku.hk | en_HK |
dc.identifier.authority | Deng, W=rp01640 | en_HK |
dc.identifier.authority | Tsao, G=rp00399 | en_HK |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1074/jbc.M109.048397 | en_HK |
dc.identifier.pmid | 20007324 | - |
dc.identifier.pmcid | PMC2836097 | - |
dc.identifier.scopus | eid_2-s2.0-77951192102 | en_HK |
dc.identifier.hkuros | 182598 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-77951192102&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 285 | en_HK |
dc.identifier.issue | 7 | en_HK |
dc.identifier.spage | 4931 | en_HK |
dc.identifier.epage | 4940 | en_HK |
dc.identifier.isi | WOS:000275274800070 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Liang, Y=26421277700 | en_HK |
dc.identifier.scopusauthorid | Zhong, Z=41763173300 | en_HK |
dc.identifier.scopusauthorid | Huang, Y=8977779900 | en_HK |
dc.identifier.scopusauthorid | Deng, W=7202223673 | en_HK |
dc.identifier.scopusauthorid | Cao, J=14520965900 | en_HK |
dc.identifier.scopusauthorid | Tsao, G=7102813116 | en_HK |
dc.identifier.scopusauthorid | Liu, Q=12809604100 | en_HK |
dc.identifier.scopusauthorid | Pei, D=7102806599 | en_HK |
dc.identifier.scopusauthorid | Kang, T=7402965550 | en_HK |
dc.identifier.scopusauthorid | Zeng, YX=7402981579 | en_HK |
dc.identifier.citeulike | 11284570 | - |
dc.identifier.issnl | 0021-9258 | - |