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Article: Efficient induction and expansion of human alloantigen-specific CD8 regulatory T cells from naive precursors by CD40-activated B cells
Title | Efficient induction and expansion of human alloantigen-specific CD8 regulatory T cells from naive precursors by CD40-activated B cells | ||||||||||||||
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Authors | |||||||||||||||
Issue Date | 2009 | ||||||||||||||
Publisher | American Association of Immunologists. The Journal's web site is located at http://www.jimmunol.org | ||||||||||||||
Citation | Journal Of Immunology, 2009, v. 183 n. 6, p. 3742-3750 How to Cite? | ||||||||||||||
Abstract | Although recent studies have focused on CD4 + regulatory T cells (Treg), CD8 + Treg have also been reported to play important roles in the induction and maintenance of immune tolerance. Adoptive transfer of CD8 + Treg in rodents or induction of CD8 + Treg in humans can prevent or treat allograft rejection and autoimmune diseases. However, no approaches have been reported for the generation of human Ag-specific CD8 + Treg at a practical scale for clinical use. Here, we found that two novel CD8 + T cell subsets with different levels of CD8 surface expression, CD8 high and CD8 low, could be induced from naive CD8 + precursors in vitro by allogeneic CD40-activated B cells, whereas only CD8 high T cells were alloantigen-specific Treg with relatively poor alloantigen-specific cytotoxicity. Importantly, alloantigen-specific CD8 high Treg could be induced and expanded from naive CD8 +CD25 - T cells at a large scale after 3 wk of culture without exogenous cytokines. These induced alloantigen-specific Treg were CD45RO + and CCR7 - memory cells, and they expressed Foxp3, CD25, CD27, CD28, and CD62L. The induction and expansion of CD8 high Treg by CD40-activated B cells were dependent on endogenously expressed IFN-γ, IL-2, IL-4, and CTLA-4. This approach may facilitate the clinical application of CD8 + Treg-based immunotherapy in transplantation and autoimmune diseases. Copyright © 2009 by The American Association of Immunologists, Inc. | ||||||||||||||
Persistent Identifier | http://hdl.handle.net/10722/125232 | ||||||||||||||
ISSN | 2023 Impact Factor: 3.6 2023 SCImago Journal Rankings: 1.558 | ||||||||||||||
ISI Accession Number ID |
Funding Information: This work was supported in part by Seed Funding for Basic Research. University Research Committee, University of Hong Kong, Hong Kong SAR, People's Republic of China (to W.T.); Edward Sai-Kim Hotung Pediatric Education and Research Fund (to Y.L.L. and W.T.); and the University of Hong Kong Postgraduate Studentships (to J.Z., G.Q.. P.L.C.. and H.M.). | ||||||||||||||
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Zheng, J | en_HK |
dc.contributor.author | Liu, Y | en_HK |
dc.contributor.author | Qin, G | en_HK |
dc.contributor.author | Chan, PL | en_HK |
dc.contributor.author | Mao, H | en_HK |
dc.contributor.author | Lam, KT | en_HK |
dc.contributor.author | Lewis, DB | en_HK |
dc.contributor.author | Lau, YL | en_HK |
dc.contributor.author | Tu, W | en_HK |
dc.date.accessioned | 2010-10-31T11:18:58Z | - |
dc.date.available | 2010-10-31T11:18:58Z | - |
dc.date.issued | 2009 | en_HK |
dc.identifier.citation | Journal Of Immunology, 2009, v. 183 n. 6, p. 3742-3750 | en_HK |
dc.identifier.issn | 0022-1767 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/125232 | - |
dc.description.abstract | Although recent studies have focused on CD4 + regulatory T cells (Treg), CD8 + Treg have also been reported to play important roles in the induction and maintenance of immune tolerance. Adoptive transfer of CD8 + Treg in rodents or induction of CD8 + Treg in humans can prevent or treat allograft rejection and autoimmune diseases. However, no approaches have been reported for the generation of human Ag-specific CD8 + Treg at a practical scale for clinical use. Here, we found that two novel CD8 + T cell subsets with different levels of CD8 surface expression, CD8 high and CD8 low, could be induced from naive CD8 + precursors in vitro by allogeneic CD40-activated B cells, whereas only CD8 high T cells were alloantigen-specific Treg with relatively poor alloantigen-specific cytotoxicity. Importantly, alloantigen-specific CD8 high Treg could be induced and expanded from naive CD8 +CD25 - T cells at a large scale after 3 wk of culture without exogenous cytokines. These induced alloantigen-specific Treg were CD45RO + and CCR7 - memory cells, and they expressed Foxp3, CD25, CD27, CD28, and CD62L. The induction and expansion of CD8 high Treg by CD40-activated B cells were dependent on endogenously expressed IFN-γ, IL-2, IL-4, and CTLA-4. This approach may facilitate the clinical application of CD8 + Treg-based immunotherapy in transplantation and autoimmune diseases. Copyright © 2009 by The American Association of Immunologists, Inc. | en_HK |
dc.language | eng | en_HK |
dc.publisher | American Association of Immunologists. The Journal's web site is located at http://www.jimmunol.org | en_HK |
dc.relation.ispartof | Journal of Immunology | en_HK |
dc.subject.mesh | B-Lymphocytes - immunology | - |
dc.subject.mesh | CD8-Positive T-Lymphocytes - cytology - immunology | - |
dc.subject.mesh | Cell Lineage - immunology | - |
dc.subject.mesh | Isoantigens - immunology | - |
dc.subject.mesh | T-Lymphocytes, Regulatory - cytology - immunology | - |
dc.title | Efficient induction and expansion of human alloantigen-specific CD8 regulatory T cells from naive precursors by CD40-activated B cells | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-1767&volume=183&issue=6&spage=3742&epage=3750&date=2009&atitle=Efficient+induction+and+expansion+of+human+alloantigen-specific+CD8+regulatory+T+cells+from+naive+precursors+by+CD40-activated+B+cells | en_HK |
dc.identifier.email | Liu, Y:yinpingl@hku.hk | en_HK |
dc.identifier.email | Mao, H:hwmau@hku.hk | en_HK |
dc.identifier.email | Lau, YL:lauylung@hkucc.hku.hk | en_HK |
dc.identifier.email | Tu, W:wwtu@hkucc.hku.hk | en_HK |
dc.identifier.authority | Liu, Y=rp00269 | en_HK |
dc.identifier.authority | Mao, H=rp01595 | en_HK |
dc.identifier.authority | Lau, YL=rp00361 | en_HK |
dc.identifier.authority | Tu, W=rp00416 | en_HK |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.4049/jimmunol.0901329 | en_HK |
dc.identifier.pmid | 19684082 | - |
dc.identifier.scopus | eid_2-s2.0-70349339392 | en_HK |
dc.identifier.hkuros | 179381 | en_HK |
dc.identifier.hkuros | 163412 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-70349339392&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 183 | en_HK |
dc.identifier.issue | 6 | en_HK |
dc.identifier.spage | 3742 | en_HK |
dc.identifier.epage | 3750 | en_HK |
dc.identifier.isi | WOS:000270179700025 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Zheng, J=55217878700 | en_HK |
dc.identifier.scopusauthorid | Liu, Y=35240639600 | en_HK |
dc.identifier.scopusauthorid | Qin, G=35085420900 | en_HK |
dc.identifier.scopusauthorid | Chan, PL=25631876900 | en_HK |
dc.identifier.scopusauthorid | Mao, H=25632489000 | en_HK |
dc.identifier.scopusauthorid | Lam, KT=25630903400 | en_HK |
dc.identifier.scopusauthorid | Lewis, DB=7404750928 | en_HK |
dc.identifier.scopusauthorid | Lau, YL=7201403380 | en_HK |
dc.identifier.scopusauthorid | Tu, W=7006479236 | en_HK |
dc.identifier.issnl | 0022-1767 | - |