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- Publisher Website: 10.1089/ten.tea.2008.0661
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Conference Paper: Effects of reconstituted collagen matrix on fates of mouse embryonic stem cells before and after induction for chondrogenic differentiation
Title | Effects of reconstituted collagen matrix on fates of mouse embryonic stem cells before and after induction for chondrogenic differentiation | ||||||||||||
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Authors | |||||||||||||
Issue Date | 2009 | ||||||||||||
Publisher | Mary Ann Liebert, Inc. Publishers. The Journal's web site is located at http://www.liebertpub.com/publication.aspx?pub_id=263 | ||||||||||||
Citation | The 7th Annual Meeting of the International Society for Stem Cell Research (ISSCR), Barcelona, Spain, 8-11 July 2009. In Tissue Engineering. Part A, 2009, v. 15 n. 10, p. 3071-3085 How to Cite? | ||||||||||||
Abstract | Embryonic stem (ES) cells are pluripotent cells with great potential in regenerative medicine. However, controlling their differentiation toward homogeneous lineages is challenging. In this study, we aim to investigate the effects of reconstituted 3D collagen matrix on the fates of mouse ES (mES) cells before and after induction for chondrogenic differentiation. Specifically, mES cells were encapsulated and cultured in 3D collagen microspheres and exposed to induction signals at different time points. Growth characteristics and differentiation status of mES cells were then evaluated. Collagen microspheres provided a suitable microenvironment supporting mES cell growth and maintaining their undifferentiated status for certain period of time. At later time points, the proportion of undifferentiated mES cells gradually decreased, accompanied by increasing proportions of mesenchymal progenitor cells. This suggests the inductive role of collagen matrix in differentiating mES cells toward mesenchymal lineages. Moreover, a lower initial collagen monomer concentration facilitated the differentiation of mES cells into chondrogenic lineages, while induction at a later time point associated with a more advanced stage of chondrogenic differentiation. This indicates that both the initial collagen concentration and the time to induce differentiation significantly affected the fates of mES cells. This study contributes to future development of ES cell-based therapies. © Copyright 2009, Mary Ann Liebert, Inc. | ||||||||||||
Persistent Identifier | http://hdl.handle.net/10722/129823 | ||||||||||||
ISSN | 2023 Impact Factor: 3.5 2023 SCImago Journal Rankings: 0.825 | ||||||||||||
ISI Accession Number ID |
Funding Information: This work was supported by grants from AOSpine (AOSBRC-07-06); the Innovation and Technology Commission, the Hong Kong Government (GHP=050=06); University Research Committee (10206799); and University's Strategic Research Theme on Development, Growth and Reproduction. The authors thank Prof. Andras Nagy for useful discussion and kind provision of the mES cells (R1), Ms. Alice Lui for technical assistance on the flow cytometry analysis, and the Department of Pediatrics and Adolescent Medicine for providing the flow cytometer for analysis. | ||||||||||||
References |
DC Field | Value | Language |
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dc.contributor.author | Yeung, CW | en_HK |
dc.contributor.author | Cheah, K | en_HK |
dc.contributor.author | Chan, D | en_HK |
dc.contributor.author | Chan, BP | en_HK |
dc.date.accessioned | 2010-12-23T08:42:29Z | - |
dc.date.available | 2010-12-23T08:42:29Z | - |
dc.date.issued | 2009 | en_HK |
dc.identifier.citation | The 7th Annual Meeting of the International Society for Stem Cell Research (ISSCR), Barcelona, Spain, 8-11 July 2009. In Tissue Engineering. Part A, 2009, v. 15 n. 10, p. 3071-3085 | en_HK |
dc.identifier.issn | 1937-3341 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/129823 | - |
dc.description.abstract | Embryonic stem (ES) cells are pluripotent cells with great potential in regenerative medicine. However, controlling their differentiation toward homogeneous lineages is challenging. In this study, we aim to investigate the effects of reconstituted 3D collagen matrix on the fates of mouse ES (mES) cells before and after induction for chondrogenic differentiation. Specifically, mES cells were encapsulated and cultured in 3D collagen microspheres and exposed to induction signals at different time points. Growth characteristics and differentiation status of mES cells were then evaluated. Collagen microspheres provided a suitable microenvironment supporting mES cell growth and maintaining their undifferentiated status for certain period of time. At later time points, the proportion of undifferentiated mES cells gradually decreased, accompanied by increasing proportions of mesenchymal progenitor cells. This suggests the inductive role of collagen matrix in differentiating mES cells toward mesenchymal lineages. Moreover, a lower initial collagen monomer concentration facilitated the differentiation of mES cells into chondrogenic lineages, while induction at a later time point associated with a more advanced stage of chondrogenic differentiation. This indicates that both the initial collagen concentration and the time to induce differentiation significantly affected the fates of mES cells. This study contributes to future development of ES cell-based therapies. © Copyright 2009, Mary Ann Liebert, Inc. | en_HK |
dc.language | eng | en_US |
dc.publisher | Mary Ann Liebert, Inc. Publishers. The Journal's web site is located at http://www.liebertpub.com/publication.aspx?pub_id=263 | en_HK |
dc.relation.ispartof | Tissue Engineering. Part A | en_HK |
dc.rights | This is a copy of an article published in the Tissue Engineering. Part A © 2009. Copyright Mary Ann Liebert, Inc. Tissue Engineering. Part A is available online at: http://www.liebertonline.com. | - |
dc.subject.mesh | Animals | - |
dc.subject.mesh | Cell Differentiation - drug effects - physiology | - |
dc.subject.mesh | Collagen - metabolism - pharmacology | - |
dc.subject.mesh | Embryonic Stem Cells - cytology - drug effects | - |
dc.subject.mesh | Extracellular Matrix - metabolism | - |
dc.title | Effects of reconstituted collagen matrix on fates of mouse embryonic stem cells before and after induction for chondrogenic differentiation | en_HK |
dc.type | Conference_Paper | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1937-3341&volume=15&issue=10&spage=3071&epage=3085&date=2009&atitle=Effects+of+reconstituted+collagen+matrix+on+fates+of+mouse+embryonic+stem+cells+before+and+after+induction+for+chondrogenic+differentiation | - |
dc.identifier.email | Cheah, K:hrmbdkc@hku.hk | en_HK |
dc.identifier.email | Chan, D:chand@hkucc.hku.hk | en_HK |
dc.identifier.email | Chan, BP:bpchan@hkucc.hku.hk | en_HK |
dc.identifier.authority | Cheah, K=rp00342 | en_HK |
dc.identifier.authority | Chan, D=rp00540 | en_HK |
dc.identifier.authority | Chan, BP=rp00087 | en_HK |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1089/ten.tea.2008.0661 | en_HK |
dc.identifier.pmid | 19331579 | - |
dc.identifier.scopus | eid_2-s2.0-73349139564 | en_HK |
dc.identifier.hkuros | 177478 | en_US |
dc.identifier.hkuros | 164807 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-73349139564&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 15 | en_HK |
dc.identifier.issue | 10 | en_HK |
dc.identifier.spage | 3071 | en_HK |
dc.identifier.epage | 3085 | en_HK |
dc.identifier.isi | WOS:000270553200028 | - |
dc.publisher.place | United States | en_HK |
dc.description.other | The 7th Annual Meeting of the International Society for Stem Cell Research (ISSCR), Barcelona, Spain, 8-11 July 2009. In Tissue Engineering. Part A, 2009, v. 15 n. 10, p. 3071-3085 | - |
dc.identifier.scopusauthorid | Yeung, CW=18635690500 | en_HK |
dc.identifier.scopusauthorid | Cheah, K=35387746200 | en_HK |
dc.identifier.scopusauthorid | Chan, D=7402216545 | en_HK |
dc.identifier.scopusauthorid | Chan, BP=7201530390 | en_HK |
dc.customcontrol.immutable | sml 170616 amended | - |
dc.identifier.issnl | 1937-3341 | - |