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- Publisher Website: 10.1002/pmic.200900846
- Scopus: eid_2-s2.0-77954709515
- PMID: 20455212
- WOS: WOS:000280615200011
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Article: Protein expression during the embryonic development of a gastropod
Title | Protein expression during the embryonic development of a gastropod | ||||||
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Authors | |||||||
Keywords | 2-DE Animal proteomics Apple snail de novo sequencing Embryonic development Gastropod | ||||||
Issue Date | 2010 | ||||||
Publisher | Wiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/proteomics | ||||||
Citation | Proteomics, 2010, v. 10 n. 14, p. 2701-2711 How to Cite? | ||||||
Abstract | Despite the potential use of gastropod embryos in basic and applied research, little is known about their protein expression. We examined, for the first time, changes in proteomic profile during embryonic development of Pomacea canaliculata from an embryo without a shell (stage II) to an embryo with a fully formed shell (stage III) to understand the roles that proteins play in critical developmental events, such as the formation of shell, operculum and heart, and the differentiation of head and foot. To analyze protein expression during development, we used 2-DE to detect, MS to analyze, and de novo peptide sequencing followed by MS-BLAST to identify the proteins. The de novo cross-species protein identification method was adopted because of a lack of genomic and proteomic data in the whole class of Gastropoda. 2-DE detected approximately 700 protein spots. Among the 125 spots that were abundant, 52% were identified, a marked improvement over the conventional direct MS-BLAST method. These proteins function in perivitelline fluid utilization, shell formation, protein synthesis and folding, and cell cycle and cell fate determination, providing evidence to support that this embryonic period is a period of dynamic protein synthesis and metabolism. The data shall provide a basis for further studies of how gastropod embryos respond to natural and human-induced changes in the environment. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA. | ||||||
Persistent Identifier | http://hdl.handle.net/10722/130016 | ||||||
ISSN | 2023 Impact Factor: 3.4 2023 SCImago Journal Rankings: 1.011 | ||||||
ISI Accession Number ID |
Funding Information: The authors thank Professor Andrej Shevchenko for his guidance on de novo protein identification, Pak Ki Wong for collecting snails from the field, Yin Ki Tam and Jason Tam for conducting mass spectrometry analyses, and two anonymous reviewers for critical comments. This study was supported by a HKBU postgraduate scholarship to J. S., a grant (HKBU FRG/08-09/II-08) to J. W. Q., and a grant (AoE/P-04/04-2-II) to P. Y. Q. | ||||||
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sun, J | en_HK |
dc.contributor.author | Zhang, Y | en_HK |
dc.contributor.author | Thiyagarajan, V | en_HK |
dc.contributor.author | Qian, PY | en_HK |
dc.contributor.author | Qiu, JW | en_HK |
dc.date.accessioned | 2010-12-23T08:45:37Z | - |
dc.date.available | 2010-12-23T08:45:37Z | - |
dc.date.issued | 2010 | en_HK |
dc.identifier.citation | Proteomics, 2010, v. 10 n. 14, p. 2701-2711 | en_HK |
dc.identifier.issn | 1615-9853 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/130016 | - |
dc.description.abstract | Despite the potential use of gastropod embryos in basic and applied research, little is known about their protein expression. We examined, for the first time, changes in proteomic profile during embryonic development of Pomacea canaliculata from an embryo without a shell (stage II) to an embryo with a fully formed shell (stage III) to understand the roles that proteins play in critical developmental events, such as the formation of shell, operculum and heart, and the differentiation of head and foot. To analyze protein expression during development, we used 2-DE to detect, MS to analyze, and de novo peptide sequencing followed by MS-BLAST to identify the proteins. The de novo cross-species protein identification method was adopted because of a lack of genomic and proteomic data in the whole class of Gastropoda. 2-DE detected approximately 700 protein spots. Among the 125 spots that were abundant, 52% were identified, a marked improvement over the conventional direct MS-BLAST method. These proteins function in perivitelline fluid utilization, shell formation, protein synthesis and folding, and cell cycle and cell fate determination, providing evidence to support that this embryonic period is a period of dynamic protein synthesis and metabolism. The data shall provide a basis for further studies of how gastropod embryos respond to natural and human-induced changes in the environment. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA. | en_HK |
dc.language | eng | en_US |
dc.publisher | Wiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/proteomics | en_HK |
dc.relation.ispartof | Proteomics | en_HK |
dc.subject | 2-DE | en_HK |
dc.subject | Animal proteomics | en_HK |
dc.subject | Apple snail | en_HK |
dc.subject | de novo sequencing | en_HK |
dc.subject | Embryonic development | en_HK |
dc.subject | Gastropod | en_HK |
dc.title | Protein expression during the embryonic development of a gastropod | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1615-9861&volume=10&issue=14&spage=2701&epage=2711&date=2010&atitle=Protein+expression+during+the+embryonic+development+of+a+gastropod | en_US |
dc.identifier.email | Thiyagarajan, V: rajan@hkucc.hku.hk | en_HK |
dc.identifier.authority | Thiyagarajan, V=rp00796 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1002/pmic.200900846 | en_HK |
dc.identifier.pmid | 20455212 | - |
dc.identifier.scopus | eid_2-s2.0-77954709515 | en_HK |
dc.identifier.hkuros | 177919 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-77954709515&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 10 | en_HK |
dc.identifier.issue | 14 | en_HK |
dc.identifier.spage | 2701 | en_HK |
dc.identifier.epage | 2711 | en_HK |
dc.identifier.isi | WOS:000280615200011 | - |
dc.publisher.place | Germany | en_HK |
dc.identifier.scopusauthorid | Sun, J=8734512600 | en_HK |
dc.identifier.scopusauthorid | Zhang, Y=36038958500 | en_HK |
dc.identifier.scopusauthorid | Thiyagarajan, V=6602476830 | en_HK |
dc.identifier.scopusauthorid | Qian, PY=35240648600 | en_HK |
dc.identifier.scopusauthorid | Qiu, JW=7403309861 | en_HK |
dc.identifier.issnl | 1615-9853 | - |