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- Publisher Website: 10.3760/cma.j.issn.0366-6999.2011.15.004
- Scopus: eid_2-s2.0-79961075103
- PMID: 21933554
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Article: Transplantation of human bone marrow-derived mesenchymal stem cells transfected with ectodysplasin for regeneration of sweat glands
Title | Transplantation of human bone marrow-derived mesenchymal stem cells transfected with ectodysplasin for regeneration of sweat glands | ||||||||||
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Authors | |||||||||||
Keywords | Bone marrow-derived mesenchymal stem cells Ectodysplasin Regeneration Sweat gland | ||||||||||
Issue Date | 2011 | ||||||||||
Publisher | Zhonghua Yixuehui. The Journal's web site is located at http://www.cmj.org/ | ||||||||||
Citation | Chinese Medical Journal, 2011, v. 124 n. 15, p. 2260-2268 How to Cite? | ||||||||||
Abstract | BACKGROUND: Patients with severe full-thickness burn injury suffer from their inability to maintain body temperature through perspiration because the complete destructed sweat glands can not be regenerated. Bone marrow-derived mesenchymal stem cells (BM-MSCs) represent an ideal stem-cell source for cell therapy because of their easy purification and multipotency. In this study, we attempted to induce human BM-MSCs to differentiate into sweat gland cells for sweat gland regeneration through ectodysplasin (EDA) gene transfection. METHODS: The dynamic expression of EDA and EDA receptor (EDAR) were firstly observed in the sweat gland formation during embryological development. After transfection with EDA expression vector, human BM-MSCs were transplanted into the injured areas of burn animal models. The regeneration of sweat glands was identified by perspiration test and immunohistochemical analysis. RESULTS: Endogenous expression of EDA and EDAR correlated with sweat gland development in human fetal skin. After EDA transfection, BM-MSC acquired a sweat-gland-cell phenotype, evidenced by their expression of sweat gland markers by flow cytometry analysis. Immunohistochemical staining revealed a markedly contribution of EDA-transfected BM-MSCs to the regeneration of sweat glands in the scalded paws. Positive rate for perspiration test for the paws treated with EDA-transfected BM-MSCs was significantly higher than those treated with BM-MSCs or EDA expression vector (P < 0.05). CONCLUSIONS: Our results confirmed the important role of EDA in the development of sweat gland. BM-MSCs transfected with EDA significantly improved the sweat-gland regeneration. This study suggests the potential application of EDA-modified MSCs for the repair and regeneration of injured skin and its appendages. | ||||||||||
Persistent Identifier | http://hdl.handle.net/10722/137499 | ||||||||||
ISSN | 2021 Impact Factor: 6.133 2020 SCImago Journal Rankings: 0.537 | ||||||||||
ISI Accession Number ID |
Funding Information: This work was supported by grants from the National Basic Science and Development Program of China (973 Program, No. 2005CB522603) and National Natural Science Foundation of China (No. 81000011, 81000835), Distinguished Young Talents in Higher Education of Guangdong (No. LYM091182009), and Shenzhen Technological R&D Foundation (No. JC201005280429A). |
DC Field | Value | Language |
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dc.contributor.author | Cai, S | en_US |
dc.contributor.author | Pan, Y | en_US |
dc.contributor.author | Han, B | en_US |
dc.contributor.author | Sun, TZ | en_US |
dc.contributor.author | Sheng, ZY | en_US |
dc.contributor.author | Fu, XB | en_US |
dc.date.accessioned | 2011-08-26T14:26:29Z | - |
dc.date.available | 2011-08-26T14:26:29Z | - |
dc.date.issued | 2011 | en_US |
dc.identifier.citation | Chinese Medical Journal, 2011, v. 124 n. 15, p. 2260-2268 | en_US |
dc.identifier.issn | 0366-6999 | - |
dc.identifier.uri | http://hdl.handle.net/10722/137499 | - |
dc.description.abstract | BACKGROUND: Patients with severe full-thickness burn injury suffer from their inability to maintain body temperature through perspiration because the complete destructed sweat glands can not be regenerated. Bone marrow-derived mesenchymal stem cells (BM-MSCs) represent an ideal stem-cell source for cell therapy because of their easy purification and multipotency. In this study, we attempted to induce human BM-MSCs to differentiate into sweat gland cells for sweat gland regeneration through ectodysplasin (EDA) gene transfection. METHODS: The dynamic expression of EDA and EDA receptor (EDAR) were firstly observed in the sweat gland formation during embryological development. After transfection with EDA expression vector, human BM-MSCs were transplanted into the injured areas of burn animal models. The regeneration of sweat glands was identified by perspiration test and immunohistochemical analysis. RESULTS: Endogenous expression of EDA and EDAR correlated with sweat gland development in human fetal skin. After EDA transfection, BM-MSC acquired a sweat-gland-cell phenotype, evidenced by their expression of sweat gland markers by flow cytometry analysis. Immunohistochemical staining revealed a markedly contribution of EDA-transfected BM-MSCs to the regeneration of sweat glands in the scalded paws. Positive rate for perspiration test for the paws treated with EDA-transfected BM-MSCs was significantly higher than those treated with BM-MSCs or EDA expression vector (P < 0.05). CONCLUSIONS: Our results confirmed the important role of EDA in the development of sweat gland. BM-MSCs transfected with EDA significantly improved the sweat-gland regeneration. This study suggests the potential application of EDA-modified MSCs for the repair and regeneration of injured skin and its appendages. | - |
dc.language | eng | en_US |
dc.publisher | Zhonghua Yixuehui. The Journal's web site is located at http://www.cmj.org/ | - |
dc.relation.ispartof | Chinese Medical Journal | en_US |
dc.subject | Bone marrow-derived mesenchymal stem cells | - |
dc.subject | Ectodysplasin | - |
dc.subject | Regeneration | - |
dc.subject | Sweat gland | - |
dc.subject.mesh | Bone Marrow Cells - cytology | - |
dc.subject.mesh | Ectodysplasins - genetics - metabolism | - |
dc.subject.mesh | Mesenchymal Stem Cell Transplantation - methods | - |
dc.subject.mesh | Mesenchymal Stem Cells - cytology - metabolism | - |
dc.subject.mesh | Sweat Glands - cytology - metabolism | - |
dc.title | Transplantation of human bone marrow-derived mesenchymal stem cells transfected with ectodysplasin for regeneration of sweat glands | en_US |
dc.type | Article | en_US |
dc.identifier.email | Cai, S: caisa@hku.hk | en_US |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.3760/cma.j.issn.0366-6999.2011.15.004 | - |
dc.identifier.pmid | 21933554 | - |
dc.identifier.scopus | eid_2-s2.0-79961075103 | - |
dc.identifier.hkuros | 191420 | en_US |
dc.identifier.volume | 124 | en_US |
dc.identifier.issue | 15 | - |
dc.identifier.spage | 2260 | en_US |
dc.identifier.epage | 2268 | en_US |
dc.identifier.isi | WOS:000294584100004 | - |
dc.publisher.place | China | - |
dc.identifier.issnl | 0366-6999 | - |