Total flavonoids and its anti-tumor activities by different solvent extract from Spatholobus suberectus

Abstract

Objective: To study the total flavonoids and anti-tumor activities of different solvent extract from Spatholobus suberectus and determine the extraction solvent and extraction method. Method: Using ethanol or acetone aqueous solution as the solvent, different extracts were prepared from S. suberectus by refluxing or ultrasonic extraction.The inhibitory activity on the proliferation of HT-29 cancer cell was determined by MTT;The content of total flavonoids and formononetin were determined by spectrophotometry and HPLC, respectively. Result : The content of total flavonoids and the cytotoxic activity were highest in the 60% ethanol 60 min-refluxing extract. Conclusion : Using 60% ethanol as extraction solvent, refluxing as extraction method, obtained extract from S. suberectus showed highest anti-tumor activity on HT-29 cancer cell. 目的:探討不同溶劑對雞血藤提取物中的總黃酮含量和抗腫瘤活性的影響。方法:分別用不同濃度的乙醇水溶液、丙酮水溶液作為提取溶劑,利用回流法、超聲提取法制備雞血藤提取物;采用四甲基噻唑藍(MTT)法測定提取物對結腸癌HT-29細胞增殖的抑制活性;采用分光光度法測定提取物中總黃酮含量;采用HPLC法測定提取物中芒柄花素含量。結果:60%乙醇回流60 min所得提取物總黃酮含量最高并且對HT-29細胞增殖的抑制活性最強。結論:雞血藤抗腫瘤活性提取物的制備以60%乙醇回流提取為佳。