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Article: Synthesis and characterization of fluorescent copolymer containing rare earth metal complex and its interaction with DNA

TitleSynthesis and characterization of fluorescent copolymer containing rare earth metal complex and its interaction with DNA
Authors
Hu, XXLi, PHYeung, KWKChu, PKWu, SLXu, ZS
Keywordsbiomaterials
DNA
fluorescent copolymer
functionalization of polymers
luminescence
rare earth
Issue Date2010
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/36444
Citation
Journal Of Polymer Science, Part A: Polymer Chemistry, 2010, v. 48 n. 24, p. 5961-5967 How to Cite?
DOI: http://dx.doi.org/10.1002/pola.24413
AbstractA complex of Eu 3+, acrylic acid (AA), and 1, 10-phenanthroline (Phen) was synthesized. The structure and fluorescence of Eu(AA) 3Phen was characterized with elemental analysis, FTIR, 1H NMR, and fluorescence spectroscopy. A novel copolymer containing rare earth complex, poly(PEGMA-co-NIPAm-co-Eu(AA)3Phen) (PPNEu), was prepared by free radical copolymerization in methanol with azodiisobutyronitrile as initiator. 1H NMR, fluorescence spectroscopy, UV-vis spectroscopy, and TEM were used to characterize this copolymer. The interaction of PPNEu with deoxyribonucleic acid (DNA) was studied by fluorescence spectroscopy, UV-vis spectroscopy and agarose gel electrophoresis. The results of fluorescence, UV-vis absorption, and agarose electrophoresis indicated that the PPNEu could interact with DNA in an electrostatic bonding mode. The TEM observation showed that the PPNEu could form spherical micelles in water solution small than 100 nm; the efficient complexation of PPNEu with DNA occurred. These results suggested the potential of the PPNEu as gene detective reagent and gene delivery carrier. © 2010 Wiley Periodicals, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/139552
ISSN
0887-624X
2021 Impact Factor: 2.869
2020 SCImago Journal Rankings: 0.768
ISI Accession Number ID
WOS:000285485700038
Funding AgencyGrant Number
National Natural Science Foun dation of China50973027
Specialized Research Fund for the Doctoral Program of Higher Education20094208110002
Funding Information:

This work is supported by the National Natural Science Foun dation of China (No 50973027) and Specialized Research Fund for the Doctoral Program of Higher Education (No 20094208110002) The authors are grateful to Gongwu Song and his group Faculty of Chemistry and Chemical Engineering, for their help in fluorescence spectra determination, Loon Ma and his group Faculty of Biology Science for their help in DNA complexation determination respectively

References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorHu, XXen_HK
dc.contributor.authorLi, PHen_HK
dc.contributor.authorYeung, KWKen_HK
dc.contributor.authorChu, PKen_HK
dc.contributor.authorWu, SLen_HK
dc.contributor.authorXu, ZSen_HK
dc.date.accessioned2011-09-23T05:51:35Z-
dc.date.available2011-09-23T05:51:35Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of Polymer Science, Part A: Polymer Chemistry, 2010, v. 48 n. 24, p. 5961-5967en_HK
dc.identifier.issn0887-624Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/139552-
dc.description.abstractA complex of Eu 3+, acrylic acid (AA), and 1, 10-phenanthroline (Phen) was synthesized. The structure and fluorescence of Eu(AA) 3Phen was characterized with elemental analysis, FTIR, 1H NMR, and fluorescence spectroscopy. A novel copolymer containing rare earth complex, poly(PEGMA-co-NIPAm-co-Eu(AA)3Phen) (PPNEu), was prepared by free radical copolymerization in methanol with azodiisobutyronitrile as initiator. 1H NMR, fluorescence spectroscopy, UV-vis spectroscopy, and TEM were used to characterize this copolymer. The interaction of PPNEu with deoxyribonucleic acid (DNA) was studied by fluorescence spectroscopy, UV-vis spectroscopy and agarose gel electrophoresis. The results of fluorescence, UV-vis absorption, and agarose electrophoresis indicated that the PPNEu could interact with DNA in an electrostatic bonding mode. The TEM observation showed that the PPNEu could form spherical micelles in water solution small than 100 nm; the efficient complexation of PPNEu with DNA occurred. These results suggested the potential of the PPNEu as gene detective reagent and gene delivery carrier. © 2010 Wiley Periodicals, Inc.en_HK
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/36444en_HK
dc.relation.ispartofJournal of Polymer Science, Part A: Polymer Chemistryen_HK
dc.rightsJournal of Polymer Science Part A, Polymer Chemistry. Copyright © John Wiley & Sons, Inc.-
dc.subjectbiomaterialsen_HK
dc.subjectDNAen_HK
dc.subjectfluorescent copolymeren_HK
dc.subjectfunctionalization of polymersen_HK
dc.subjectluminescenceen_HK
dc.subjectrare earthen_HK
dc.titleSynthesis and characterization of fluorescent copolymer containing rare earth metal complex and its interaction with DNAen_HK
dc.typeArticleen_HK
dc.identifier.emailYeung, KWK:wkkyeung@hkucc.hku.hken_HK
dc.identifier.authorityYeung, KWK=rp00309en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/pola.24413en_HK
dc.identifier.scopuseid_2-s2.0-78649537645en_HK
dc.identifier.hkuros192185en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78649537645&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume48en_HK
dc.identifier.issue24en_HK
dc.identifier.spage5961en_HK
dc.identifier.epage5967en_HK
dc.identifier.isiWOS:000285485700038-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHu, XX=8650009700en_HK
dc.identifier.scopusauthoridLi, PH=7404773164en_HK
dc.identifier.scopusauthoridYeung, KWK=13309584700en_HK
dc.identifier.scopusauthoridChu, PK=36040705700en_HK
dc.identifier.scopusauthoridWu, SL=36678457200en_HK
dc.identifier.scopusauthoridXu, ZS=12760201200en_HK
dc.identifier.issnl0887-624X-

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