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- Publisher Website: 10.2500/ajra.2011.25.3604
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- PMID: 21679501
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Article: M 1and M 3 muscarinic receptors mediate relaxation and contraction in canine nasal veins
Title | M 1and M 3 muscarinic receptors mediate relaxation and contraction in canine nasal veins | ||||
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Authors | |||||
Issue Date | 2011 | ||||
Publisher | OceanSide Publications, Inc. The Journal's web site is located at http://www.oceansidepubl.com/ajr/index.htm | ||||
Citation | American Journal Of Rhinology And Allergy, 2011, v. 25 n. 2, p. e60-e65 How to Cite? | ||||
Abstract | Background: Acetylcholine (ACh) has been shown to induce nasal congestion via vasorelaxation of intranasal posterior collecting veins (PCV) coupled with vasocontraction of extranasal outflow veins (dorsal nasal vein [DNV] and sphenopalatine vein [SPV]). The aim of this study was to characterize the muscarinic receptor subtype(s) involved in ACh-induced relaxation and contraction in canine nasal veins. Methods: PCV, DNV, and SPV were isolated from the canine nose. In vitro isometric tension of segments from these veins was monitored to reflect vascular reactivity. ACh concentration-response curve was studied in the presence of muscarinic receptor subtype inhibitors. Immunohistochemical localization of M 1-M 5 receptor subtypes in the veins was performed. Results: ACh-induced relaxation in PVC was inhibited by pertussis toxin (PTX; inhibitor of G-protein that couples M2/M4 receptors), methoctramine (selective M 2 muscarinic receptor inhibitor), muscarinic toxin 7 (MT-7; selective M 1 muscarinic receptor inhibitor), and 4-diphenylacetoxy-methylpiperidine methiodide (4-DAMP; selective M3 muscarinic receptor inhibitor). ACh-induced contraction in SPV and DNV was potentiated by PTX and methoctramine but was inhibited by MT-7 and 4-DAMP. Immunohistochemistry confirmed the presence of five muscarinic receptor subtypes in the endothelium of nasal veins, with staining of M 3 > M 1 > M 5 > M 2 > M 4 in PVC but M 2 > M 4 > M 3 > M 1 > M 5 in outflow veins. M1 and M3 receptor subtypes were localized in the smooth muscles of both types of veins. Conclusion: The results show that ACh relaxes intranasal veins and contracts extranasal veins primarily via M 1 and M 3 muscarinic receptor subtypes, implying the therapeutic value of M 1/M 3-specific or highly selective anticholinergics on nasal congestion. Copyright © 2011, OceanSide Publications, Inc. | ||||
Persistent Identifier | http://hdl.handle.net/10722/139663 | ||||
ISSN | 2023 Impact Factor: 2.5 2023 SCImago Journal Rankings: 0.709 | ||||
ISI Accession Number ID |
Funding Information: Supported by CRCG Grant 200607176113 from The University of Hong Kong | ||||
References | |||||
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DC Field | Value | Language |
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dc.contributor.author | Lung, MA | en_HK |
dc.date.accessioned | 2011-09-23T05:53:10Z | - |
dc.date.available | 2011-09-23T05:53:10Z | - |
dc.date.issued | 2011 | en_HK |
dc.identifier.citation | American Journal Of Rhinology And Allergy, 2011, v. 25 n. 2, p. e60-e65 | en_HK |
dc.identifier.issn | 1945-8924 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/139663 | - |
dc.description.abstract | Background: Acetylcholine (ACh) has been shown to induce nasal congestion via vasorelaxation of intranasal posterior collecting veins (PCV) coupled with vasocontraction of extranasal outflow veins (dorsal nasal vein [DNV] and sphenopalatine vein [SPV]). The aim of this study was to characterize the muscarinic receptor subtype(s) involved in ACh-induced relaxation and contraction in canine nasal veins. Methods: PCV, DNV, and SPV were isolated from the canine nose. In vitro isometric tension of segments from these veins was monitored to reflect vascular reactivity. ACh concentration-response curve was studied in the presence of muscarinic receptor subtype inhibitors. Immunohistochemical localization of M 1-M 5 receptor subtypes in the veins was performed. Results: ACh-induced relaxation in PVC was inhibited by pertussis toxin (PTX; inhibitor of G-protein that couples M2/M4 receptors), methoctramine (selective M 2 muscarinic receptor inhibitor), muscarinic toxin 7 (MT-7; selective M 1 muscarinic receptor inhibitor), and 4-diphenylacetoxy-methylpiperidine methiodide (4-DAMP; selective M3 muscarinic receptor inhibitor). ACh-induced contraction in SPV and DNV was potentiated by PTX and methoctramine but was inhibited by MT-7 and 4-DAMP. Immunohistochemistry confirmed the presence of five muscarinic receptor subtypes in the endothelium of nasal veins, with staining of M 3 > M 1 > M 5 > M 2 > M 4 in PVC but M 2 > M 4 > M 3 > M 1 > M 5 in outflow veins. M1 and M3 receptor subtypes were localized in the smooth muscles of both types of veins. Conclusion: The results show that ACh relaxes intranasal veins and contracts extranasal veins primarily via M 1 and M 3 muscarinic receptor subtypes, implying the therapeutic value of M 1/M 3-specific or highly selective anticholinergics on nasal congestion. Copyright © 2011, OceanSide Publications, Inc. | en_HK |
dc.language | eng | en_US |
dc.publisher | OceanSide Publications, Inc. The Journal's web site is located at http://www.oceansidepubl.com/ajr/index.htm | en_HK |
dc.relation.ispartof | American Journal of Rhinology and Allergy | en_HK |
dc.subject.mesh | Acetylcholine - pharmacology | - |
dc.subject.mesh | Nasal Obstruction - drug therapy | - |
dc.subject.mesh | Receptor, Muscarinic M1 - antagonists and inhibitors - metabolism | - |
dc.subject.mesh | Receptor, Muscarinic M3 - antagonists and inhibitors - metabolism | - |
dc.subject.mesh | Veins - drug effects - metabolism - pathology | - |
dc.title | M 1and M 3 muscarinic receptors mediate relaxation and contraction in canine nasal veins | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Lung, MA: makylung@hkucc.hku.hk | en_HK |
dc.identifier.authority | Lung, MA=rp00319 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.2500/ajra.2011.25.3604 | en_HK |
dc.identifier.pmid | 21679501 | - |
dc.identifier.scopus | eid_2-s2.0-79953248653 | en_HK |
dc.identifier.hkuros | 194223 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-79953248653&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 25 | en_HK |
dc.identifier.issue | 2 | en_HK |
dc.identifier.spage | e60 | en_HK |
dc.identifier.epage | e65 | en_HK |
dc.identifier.eissn | 1945-8932 | - |
dc.identifier.isi | WOS:000292636400003 | - |
dc.publisher.place | United States | en_HK |
dc.relation.project | Muscarinic receptor subtypes in the control of nasal patency | - |
dc.identifier.scopusauthorid | Lung, MA=7006411781 | en_HK |
dc.identifier.issnl | 1945-8932 | - |