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Article: Substitution of lysine at 627 position in PB2 protein does not change virulence of the 2009 pandemic H1N1 virus in mice

TitleSubstitution of lysine at 627 position in PB2 protein does not change virulence of the 2009 pandemic H1N1 virus in mice
Authors
Zhu, HWang, JWang, PSong, WZheng, ZChen, RGuo, KZhang, TPeiris, JSMChen, HGuan, Y
KeywordsInfluenza virus
Pathogenicity
PB2 627 residue
Temperature-sensitivity
Virulence
Issue Date2010
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yviro
Citation
Virology, 2010, v. 401 n. 1, p. 1-5 How to Cite?
DOI: http://dx.doi.org/10.1016/j.virol.2010.02.024
AbstractA lysine at the 627 position (627K) of PB2 protein of influenza virus has been recognized as a determinant for host adaptation and a virulent element for some influenza viruses. While seasonal influenza viruses exclusively contained 627K, the pandemic (H1N1) 2009 possessed a glutamic acid (627E), even after circulation in humans for more than 6. months. To explore the potential role of E627K substitution in PB2 in the pandemic (H1N1) 2009 virus, we compared pathogenicity and growth properties between a recombinant virus containing 627K PB2 gene and the parental A/California/4/2009 strain containing 627E. Our results showed that substitution of 627K in PB2 gene does not confer higher virulence and growth rate for the pandemic (H1N1) 2009 virus in mice and cell culture respectively, suggesting 627K is not required for human adaptation of the pandemic (H1N1) 2009 virus. © 2010 Elsevier Inc.
Persistent Identifierhttp://hdl.handle.net/10722/141725
ISSN
0042-6822
2023 Impact Factor: 2.8
2023 SCImago Journal Rankings: 0.838
PubMed Central ID
PMC4034467
ISI Accession Number ID
WOS:000276575000001
Funding AgencyGrant Number
Li Ka Shing Foundation
University Grants Committee of the Hong Kong SARAoE/M-12/06
Research Grant Council of Hong Kong SARHKU 7488/05M
7500/06M
National Institutes of Health(NIH)HSN266200700005C
Funding Information:

We gratefully acknowledge our colleagues from IIII and SKLEID for their excellent technical assistance. This study was supported by the Li Ka Shing Foundation, the Area of Excellence Scheme of the University Grants Committee of the Hong Kong SAR (grant AoE/M-12/06), the Research Grant Council of Hong Kong SAR (HKU 7488/05M and 7500/06M), and the National Institutes of Health(NIH, National Institute of Allergy and Infectious Diseases contract HSN266200700005C).

References
References in Scopus
Grants
Control of Pandemic and Inter-pandemic Influenza
Molecular basis of genesis and determinants for pathogenicity of avian influenza H5N1 virus genotype Z

 

DC FieldValueLanguage
dc.contributor.authorZhu, Hen_HK
dc.contributor.authorWang, Jen_HK
dc.contributor.authorWang, Pen_HK
dc.contributor.authorSong, Wen_HK
dc.contributor.authorZheng, Zen_HK
dc.contributor.authorChen, Ren_HK
dc.contributor.authorGuo, Ken_HK
dc.contributor.authorZhang, Ten_HK
dc.contributor.authorPeiris, JSMen_HK
dc.contributor.authorChen, Hen_HK
dc.contributor.authorGuan, Yen_HK
dc.date.accessioned2011-09-27T02:59:28Z-
dc.date.available2011-09-27T02:59:28Z-
dc.date.issued2010en_HK
dc.identifier.citationVirology, 2010, v. 401 n. 1, p. 1-5en_HK
dc.identifier.issn0042-6822en_HK
dc.identifier.urihttp://hdl.handle.net/10722/141725-
dc.description.abstractA lysine at the 627 position (627K) of PB2 protein of influenza virus has been recognized as a determinant for host adaptation and a virulent element for some influenza viruses. While seasonal influenza viruses exclusively contained 627K, the pandemic (H1N1) 2009 possessed a glutamic acid (627E), even after circulation in humans for more than 6. months. To explore the potential role of E627K substitution in PB2 in the pandemic (H1N1) 2009 virus, we compared pathogenicity and growth properties between a recombinant virus containing 627K PB2 gene and the parental A/California/4/2009 strain containing 627E. Our results showed that substitution of 627K in PB2 gene does not confer higher virulence and growth rate for the pandemic (H1N1) 2009 virus in mice and cell culture respectively, suggesting 627K is not required for human adaptation of the pandemic (H1N1) 2009 virus. © 2010 Elsevier Inc.en_HK
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yviroen_HK
dc.relation.ispartofVirologyen_HK
dc.subjectInfluenza virusen_HK
dc.subjectPathogenicityen_HK
dc.subjectPB2 627 residueen_HK
dc.subjectTemperature-sensitivityen_HK
dc.subjectVirulenceen_HK
dc.subject.meshAmino Acid Substitutionen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCell Lineen_HK
dc.subject.meshDisease Outbreaksen_HK
dc.subject.meshDogsen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHumansen_HK
dc.subject.meshInfluenza A Virus, H1N1 Subtype - genetics - pathogenicityen_HK
dc.subject.meshInfluenza, Human - epidemiology - virologyen_HK
dc.subject.meshLysine - geneticsen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Inbred BALB Cen_HK
dc.subject.meshViral Proteins - geneticsen_HK
dc.subject.meshVirulence - geneticsen_HK
dc.subject.meshVirus Replicationen_HK
dc.titleSubstitution of lysine at 627 position in PB2 protein does not change virulence of the 2009 pandemic H1N1 virus in miceen_HK
dc.typeArticleen_HK
dc.identifier.emailZhu, H: zhuhch@hku.hken_HK
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_HK
dc.identifier.emailChen, H: hlchen@hku.hken_HK
dc.identifier.emailGuan, Y: yguan@hkucc.hku.hken_HK
dc.identifier.authorityZhu, H=rp01535en_HK
dc.identifier.authorityPeiris, JSM=rp00410en_HK
dc.identifier.authorityChen, H=rp00383en_HK
dc.identifier.authorityGuan, Y=rp00397en_HK
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1016/j.virol.2010.02.024en_HK
dc.identifier.pmid20303563-
dc.identifier.pmcidPMC4034467-
dc.identifier.scopuseid_2-s2.0-77950949967en_HK
dc.identifier.hkuros171990-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77950949967&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume401en_HK
dc.identifier.issue1en_HK
dc.identifier.spage1en_HK
dc.identifier.epage5en_HK
dc.identifier.eissn1096-0341-
dc.identifier.isiWOS:000276575000001-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectControl of Pandemic and Inter-pandemic Influenza-
dc.relation.projectMolecular basis of genesis and determinants for pathogenicity of avian influenza H5N1 virus genotype Z-
dc.identifier.scopusauthoridZhu, H=25724029300en_HK
dc.identifier.scopusauthoridWang, J=35228130300en_HK
dc.identifier.scopusauthoridWang, P=13907209900en_HK
dc.identifier.scopusauthoridSong, W=23490429800en_HK
dc.identifier.scopusauthoridZheng, Z=35747739800en_HK
dc.identifier.scopusauthoridChen, R=35745563500en_HK
dc.identifier.scopusauthoridGuo, K=25623179500en_HK
dc.identifier.scopusauthoridZhang, T=35747659600en_HK
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_HK
dc.identifier.scopusauthoridChen, H=26643315400en_HK
dc.identifier.scopusauthoridGuan, Y=7202924055en_HK
dc.identifier.citeulike6906216-
dc.identifier.issnl0042-6822-

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