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Article: Subcellular localization of SUN2 is regulated by lamin a and Rab5

TitleSubcellular localization of SUN2 is regulated by lamin a and Rab5
Authors
Liang, YChiu, PHYip, KYChan, SY
Issue Date2011
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
Plos One, 2011, v. 6 n. 5 How to Cite?
DOI: http://dx.doi.org/10.1371/journal.pone.0020507
AbstractSUN2 is an inner nuclear membrane protein with a conserved Sad1/UNC-84 homology SUN-domain at the C-terminus. Intriguingly, SUN2 has also been reported to interact with Rab5, which localizes in early endosomes. To clarify the dual subcellular localization of SUN2, we investigated its localization in lamin A/C deficient cells rescued with lamin A or lamin C isoform, and in HeLa cells transfected with Rab5 or its mutants. We found that expression of lamin A but not lamin C partly restored the nuclear envelope localization of SUN2. SUN2 was redistributed to endosomes upon overexpression of Rab5, but remained on the nuclear envelope when the SUN domain was deleted. To explore the physiological function of SUN2 in vesicle trafficking and endocytosis, we demonstrated the colocalization of endogenous SUN2 and Rab5. Moreover, overexpression of SUN2 stimulated the uptake of transferrin while suppression of SUN2 expression attenuated the process. These findings support a role of SUN2 in endocytosis. © 2011 Liang et al.
Persistent Identifierhttp://hdl.handle.net/10722/142453
ISSN
1932-6203
2021 Impact Factor: 3.752
2020 SCImago Journal Rankings: 0.990
PubMed Central ID
PMC3105078
ISI Accession Number ID
WOS:000291097600084
Funding AgencyGrant Number
Research Grants Council of the Hong Kong Special Administrative Region, ChinaHKU 7176/01M
Edward Sai-Kim Hotung Paediatric Education and Research Fund
Faculty Core Imaging Facility of the Li Ka Shing Faculty of Medicine, the University of Hong Kong
Funding Information:

This work was supported by a grant from the Research Grants Council of the Hong Kong Special Administrative Region, China (Project No. HKU 7176/01M) and Edward Sai-Kim Hotung Paediatric Education and Research Fund. This work was also supported by the Faculty Core Imaging Facility of the Li Ka Shing Faculty of Medicine, the University of Hong Kong. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

References
References in Scopus
Grants
Characterisation of three new genes expressed in human heart

 

DC FieldValueLanguage
dc.contributor.authorLiang, Yen_HK
dc.contributor.authorChiu, PHen_HK
dc.contributor.authorYip, KYen_HK
dc.contributor.authorChan, SYen_HK
dc.date.accessioned2011-10-28T02:46:20Z-
dc.date.available2011-10-28T02:46:20Z-
dc.date.issued2011en_HK
dc.identifier.citationPlos One, 2011, v. 6 n. 5en_HK
dc.identifier.issn1932-6203en_HK
dc.identifier.urihttp://hdl.handle.net/10722/142453-
dc.description.abstractSUN2 is an inner nuclear membrane protein with a conserved Sad1/UNC-84 homology SUN-domain at the C-terminus. Intriguingly, SUN2 has also been reported to interact with Rab5, which localizes in early endosomes. To clarify the dual subcellular localization of SUN2, we investigated its localization in lamin A/C deficient cells rescued with lamin A or lamin C isoform, and in HeLa cells transfected with Rab5 or its mutants. We found that expression of lamin A but not lamin C partly restored the nuclear envelope localization of SUN2. SUN2 was redistributed to endosomes upon overexpression of Rab5, but remained on the nuclear envelope when the SUN domain was deleted. To explore the physiological function of SUN2 in vesicle trafficking and endocytosis, we demonstrated the colocalization of endogenous SUN2 and Rab5. Moreover, overexpression of SUN2 stimulated the uptake of transferrin while suppression of SUN2 expression attenuated the process. These findings support a role of SUN2 in endocytosis. © 2011 Liang et al.en_HK
dc.languageengen_US
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.actionen_HK
dc.relation.ispartofPLoS ONEen_HK
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subject.meshImmunohistochemistry-
dc.subject.meshIntracellular Signaling Peptides and Proteins - genetics - metabolism-
dc.subject.meshLamin Type A - genetics - metabolism-
dc.subject.meshMembrane Proteins - genetics - metabolism-
dc.subject.meshRab5 GTP-Binding Proteins - genetics - metabolism-
dc.titleSubcellular localization of SUN2 is regulated by lamin a and Rab5en_HK
dc.typeArticleen_HK
dc.identifier.emailChan, SY:sychan@hkucc.hku.hken_HK
dc.identifier.authorityChan, SY=rp00356en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pone.0020507en_HK
dc.identifier.pmid21655223-
dc.identifier.pmcidPMC3105078-
dc.identifier.scopuseid_2-s2.0-79957838252en_HK
dc.identifier.hkuros196750en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79957838252&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume6en_HK
dc.identifier.issue5en_HK
dc.identifier.spagee20507en_US
dc.identifier.epagee20507en_US
dc.identifier.eissn1932-6203-
dc.identifier.isiWOS:000291097600084-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectCharacterisation of three new genes expressed in human heart-
dc.identifier.scopusauthoridLiang, Y=41561746000en_HK
dc.identifier.scopusauthoridChiu, PH=41560901300en_HK
dc.identifier.scopusauthoridYip, KY=41562461100en_HK
dc.identifier.scopusauthoridChan, SY=7404255082en_HK
dc.identifier.issnl1932-6203-

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