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Article: LIM domain protein FHL1B interacts with PP2A catalytic β subunit - A novel cell cycle regulatory pathway

TitleLIM domain protein FHL1B interacts with PP2A catalytic β subunit - A novel cell cycle regulatory pathway
Authors
Wong, CHFung, YWWNg, EKOLee, SMYWaye, MMYTsui, SKW
KeywordsCell cycle
Co-immunoprecipitation
Regulatory pathway
Yeast two-hybrid screening
Issue Date2010
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/febslet
Citation
Febs Letters, 2010, v. 584 n. 22, p. 4511-4516 How to Cite?
DOI: http://dx.doi.org/10.1016/j.febslet.2010.10.022
AbstractFour-and-a-half LIM domain protein 1B (FHL1B) is an alternatively-spliced isoform of FHL1. In this study, FHL1B was demonstrated to interact with the β catalytic subunit (Cβ) of a type 2A protein phosphatase (PP2A) by yeast two-hybrid screening. Domain studies using a small-scale yeast two-hybrid interaction assay revealed the mediation of protein-protein interaction by FHL1B's C-terminus. Interaction between FHL1B and PP2A was further verified by co-immunoprecipitation. FHL1B was also shown to shuttle between nucleus and cytoplasm at different phases of the cell cycle. These data suggest that the FHL1B/PP2A Cβ interaction may illustrate a novel cell-cycle regulatory pathway. Structured summary: MINT- 8044739: FHL1B (uniprotkb: Q13642-2) physically interacts (MI: 0915) with PP2Acbeta (uniprotkb: P62714) by two hybrid (MI: 0018). MINT- 8044769, MINT- 8044778: FHL1B (uniprotkb: Q13642-2) physically interacts (MI: 0915) with PP2Acbeta (uniprotkb: P62714) by anti bait coimmunoprecipitation (MI: 0006). © 2010 Federation of European Biochemical Societies.
Persistent Identifierhttp://hdl.handle.net/10722/142523
ISSN
0014-5793
2021 Impact Factor: 3.864
2020 SCImago Journal Rankings: 1.593
ISI Accession Number ID
WOS:000284148000004
Funding AgencyGrant Number
Research Grants Council, Hong KongCUHK4116/00 M
Croucher Foundation
Funding Information:

This study was supported by earmarked grants from the Research Grants Council, Hong Kong (Ref. No.: CUHK4116/00 M) and the Croucher Foundation. We would like to thank Dr. David Wilmshurst for editing a draft of this paper.

References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorWong, CHen_HK
dc.contributor.authorFung, YWWen_HK
dc.contributor.authorNg, EKOen_HK
dc.contributor.authorLee, SMYen_HK
dc.contributor.authorWaye, MMYen_HK
dc.contributor.authorTsui, SKWen_HK
dc.date.accessioned2011-10-28T02:50:25Z-
dc.date.available2011-10-28T02:50:25Z-
dc.date.issued2010en_HK
dc.identifier.citationFebs Letters, 2010, v. 584 n. 22, p. 4511-4516en_HK
dc.identifier.issn0014-5793en_HK
dc.identifier.urihttp://hdl.handle.net/10722/142523-
dc.description.abstractFour-and-a-half LIM domain protein 1B (FHL1B) is an alternatively-spliced isoform of FHL1. In this study, FHL1B was demonstrated to interact with the β catalytic subunit (Cβ) of a type 2A protein phosphatase (PP2A) by yeast two-hybrid screening. Domain studies using a small-scale yeast two-hybrid interaction assay revealed the mediation of protein-protein interaction by FHL1B's C-terminus. Interaction between FHL1B and PP2A was further verified by co-immunoprecipitation. FHL1B was also shown to shuttle between nucleus and cytoplasm at different phases of the cell cycle. These data suggest that the FHL1B/PP2A Cβ interaction may illustrate a novel cell-cycle regulatory pathway. Structured summary: MINT- 8044739: FHL1B (uniprotkb: Q13642-2) physically interacts (MI: 0915) with PP2Acbeta (uniprotkb: P62714) by two hybrid (MI: 0018). MINT- 8044769, MINT- 8044778: FHL1B (uniprotkb: Q13642-2) physically interacts (MI: 0915) with PP2Acbeta (uniprotkb: P62714) by anti bait coimmunoprecipitation (MI: 0006). © 2010 Federation of European Biochemical Societies.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/febsleten_HK
dc.relation.ispartofFEBS Lettersen_HK
dc.subjectCell cycleen_HK
dc.subjectCo-immunoprecipitationen_HK
dc.subjectRegulatory pathwayen_HK
dc.subjectYeast two-hybrid screeningen_HK
dc.subject.meshCatalytic Domain-
dc.subject.meshCell Cycle-
dc.subject.meshIntracellular Signaling Peptides and Proteins - genetics - metabolism-
dc.subject.meshMuscle Proteins - genetics - metabolism-
dc.subject.meshProtein Phosphatase 2 - chemistry - metabolism-
dc.titleLIM domain protein FHL1B interacts with PP2A catalytic β subunit - A novel cell cycle regulatory pathwayen_HK
dc.typeArticleen_HK
dc.identifier.emailNg, EKO: ngko@hku.hken_HK
dc.identifier.authorityNg, EKO=rp01364en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.febslet.2010.10.022en_HK
dc.identifier.pmid20969868-
dc.identifier.scopuseid_2-s2.0-78249236209en_HK
dc.identifier.hkuros184332en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78249236209&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume584en_HK
dc.identifier.issue22en_HK
dc.identifier.spage4511en_HK
dc.identifier.epage4516en_HK
dc.identifier.isiWOS:000284148000004-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridWong, CH=8942307600en_HK
dc.identifier.scopusauthoridFung, YWW=54890939200en_HK
dc.identifier.scopusauthoridNg, EKO=21135553700en_HK
dc.identifier.scopusauthoridLee, SMY=35233892600en_HK
dc.identifier.scopusauthoridWaye, MMY=7006687733en_HK
dc.identifier.scopusauthoridTsui, SKW=7004961364en_HK
dc.identifier.citeulike8070217-
dc.identifier.issnl0014-5793-

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