Correlation Of Estrogen Receptor Alpha Gene Polymorphisms And Bone Mineral Density In Chinese Women With Chronic Periodontitis

Abstract

Background Periodontitis And Osteoporosis Are One Of The Frequently Encountered Diseases In Post-Menopausal Women. Estrogen Receptors (Ers) Regulated Bone Metabolism. To Investigate The Possible Effect Of Er-Alpha (Α) Gene Polymorphisms On Bone Mineral Density (Bmd) In Pre- And Post-Menopausal Chinese Women With Chronic Periodontitis (Cp), We Provided Sufficient Quantitative Information Concerning The Correlation Between Er Gene Polymorphisms And Bmd In Periodontitis. Methods Sixty-Five Post-Menopausal And Eighty Pre-Menopausal Cp Women, And Sixty Post-Menopausal Healthy Individuals Were Recruited In This Study. Genomic Dna Was Extracted From Oral Mucosa Swab Sample Of Each Subject By The Chelex-100 Method. Determination Of The Er-Α Polymorphisms Was Performed By Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (Pcr-Rflp) Technique With Xbai And Pvuii Enzyme. The Index For Periodontal Examination Includes Clinical Attachment Loss (Cal) And Probing Pocket Depth (Ppd). Bmd Was Measured By Dual-Energy X-Ray Absorptiometry (Dexa). Results There Were No Significant Differences Between The Er-Α Genotypes Of Pvuii And Xbai And Bmd In Post-Menopausal And Pre-Menopausal Cp Patients, Respectively (P >0.05). However, There Was Association Between Preand Post-Menopausal Cp Patients At Bmd Of Lumbar Spine L2-L4 (P=0.027) And Ward's Bmd (P=0.004). Furthermore, The Post-Menopausal Cp Women Who Carried Pvuii Tt Genotype Presented Significantly Lower Ward's Bmd Than The Pre-Menopausal Cp Women (P=0.007), Meanwhile, The Post-Menopausal Cp Women Who Carried Xbai Aa Genotype Presented Significantly Lower Spine L2-L4 Bmd Than The Pre-Menopausal Cp Women (P=0.003). Conclusions Er-Α Gene Polymorphisms May Be A Susceptible Indicator For Bmd Variation Of Lumbar Spine L2-L4 And Ward In Chinese Pre- And Post-Menopausal Women Patients With Cp.