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Article: Human sperm binding is mediated by the sialyl-lewis x Oligosaccharide on the zona pellucida

TitleHuman sperm binding is mediated by the sialyl-lewis x Oligosaccharide on the zona pellucida
Authors
Pang, PCChiu, PCNLee, CLChang, LYPanico, MMorris, HRHaslam, SMKhoo, KHClark, GFYeung, WSBDell, A
Issue Date2011
PublisherAmerican Association for the Advancement of Science. The Journal's web site is located at http://sciencemag.org
Citation
Science, 2011, v. 333 n. 6050, p. 1761-1764 How to Cite?
DOI: http://dx.doi.org/10.1126/science.1207438
AbstractHuman fertilization begins when spermatozoa bind to the extracellular matrix coating of the oocyte, known as the zona pellucida (ZP). One spermatozoan then penetrates this matrix and fuses with the egg cell, generating a zygote. Although carbohydrate sequences on the ZP have been implicated in sperm binding, the nature of the ligand was unknown. Here, ultrasensitive mass spectrometric analyses revealed that the sialyl-Lewis x sequence [NeuAcα2-3Galβ1-4(Fucα1-3)GlcNAc], a well-known selectin ligand, is the most abundant terminal sequence on the N- and O-glycans of human ZP. Sperm-ZP binding was largely inhibited by glycoconjugates terminated with sialyl-Lewis x sequences or by antibodies directed against this sequence. Thus, the sialyl-Lewis xsequence represents the major carbohydrate ligand for human sperm-egg binding.
Persistent Identifierhttp://hdl.handle.net/10722/144614
ISSN
0036-8075
2023 Impact Factor: 44.7
2023 SCImago Journal Rankings: 11.902
ISI Accession Number ID
WOS:000295121500047
Funding AgencyGrant Number
Biotechnology and Biological Sciences Research Council (BBSRC)BBF0083091
Royal Society
Breeden-Adams Foundation
Life Sciences Mission Enhancement Reproductive Biology Program
state of Missouri
University Research Committee, University of Hong Kong
Taiwan National Science CouncilNSC99-3112-B-001-025
Funding Information:

This work was supported by the Biotechnology and Biological Sciences Research Council (BBSRC), grant BBF0083091 (to A. D., H. R. M., and S. M. H.); a Royal Society International Joint Project Award (to A. D. and K-H. K.); the Breeden-Adams Foundation and a Life Sciences Mission Enhancement Reproductive Biology Program funded by the state of Missouri (to G. F. C.); and the University Research Committee, University of Hong Kong (to P.C.N. and W.S.B.Y). The proteomics and sulfoglycomics data were acquired at the National Research Program for Genomic Medicine (NRPGM) Core Facilities for Proteomics and Glycomics funded by the Taiwan National Science Council (NSC99-3112-B-001-025). An intellectual property disclosure has been filed with the University of Missouri that covers technology described in this paper.

References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorPang, PCen_HK
dc.contributor.authorChiu, PCNen_HK
dc.contributor.authorLee, CLen_HK
dc.contributor.authorChang, LYen_HK
dc.contributor.authorPanico, Men_HK
dc.contributor.authorMorris, HRen_HK
dc.contributor.authorHaslam, SMen_HK
dc.contributor.authorKhoo, KHen_HK
dc.contributor.authorClark, GFen_HK
dc.contributor.authorYeung, WSBen_HK
dc.contributor.authorDell, Aen_HK
dc.date.accessioned2012-02-03T06:15:54Z-
dc.date.available2012-02-03T06:15:54Z-
dc.date.issued2011en_HK
dc.identifier.citationScience, 2011, v. 333 n. 6050, p. 1761-1764en_HK
dc.identifier.issn0036-8075en_HK
dc.identifier.urihttp://hdl.handle.net/10722/144614-
dc.description.abstractHuman fertilization begins when spermatozoa bind to the extracellular matrix coating of the oocyte, known as the zona pellucida (ZP). One spermatozoan then penetrates this matrix and fuses with the egg cell, generating a zygote. Although carbohydrate sequences on the ZP have been implicated in sperm binding, the nature of the ligand was unknown. Here, ultrasensitive mass spectrometric analyses revealed that the sialyl-Lewis x sequence [NeuAcα2-3Galβ1-4(Fucα1-3)GlcNAc], a well-known selectin ligand, is the most abundant terminal sequence on the N- and O-glycans of human ZP. Sperm-ZP binding was largely inhibited by glycoconjugates terminated with sialyl-Lewis x sequences or by antibodies directed against this sequence. Thus, the sialyl-Lewis xsequence represents the major carbohydrate ligand for human sperm-egg binding.en_HK
dc.languageengen_US
dc.publisherAmerican Association for the Advancement of Science. The Journal's web site is located at http://sciencemag.orgen_HK
dc.relation.ispartofScienceen_HK
dc.rightsScience. Copyright © American Association for the Advancement of Science.en_US
dc.subject.meshEgg Proteins - chemistry - metabolism-
dc.subject.meshMembrane Glycoproteins - chemistry - metabolism-
dc.subject.meshOligosaccharides - chemistry - immunology - metabolism-
dc.subject.meshReceptors, Cell Surface - chemistry - metabolism-
dc.subject.meshSperm-Ovum Interactions-
dc.titleHuman sperm binding is mediated by the sialyl-lewis x Oligosaccharide on the zona pellucidaen_HK
dc.typeArticleen_HK
dc.identifier.emailChiu, PCN:pchiucn@hku.hken_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityChiu, PCN=rp00424en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1126/science.1207438en_HK
dc.identifier.pmid21852454-
dc.identifier.scopuseid_2-s2.0-80053133500en_HK
dc.identifier.hkuros198173en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80053133500&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume333en_HK
dc.identifier.issue6050en_HK
dc.identifier.spage1761en_HK
dc.identifier.epage1764en_HK
dc.identifier.eissn1095-9203-
dc.identifier.isiWOS:000295121500047-
dc.publisher.placeUnited Statesen_HK
dc.identifier.f100013396969-
dc.identifier.scopusauthoridPang, PC=23028555800en_HK
dc.identifier.scopusauthoridChiu, PCN=25959969200en_HK
dc.identifier.scopusauthoridLee, CL=53865228300en_HK
dc.identifier.scopusauthoridChang, LY=37044638400en_HK
dc.identifier.scopusauthoridPanico, M=7005321077en_HK
dc.identifier.scopusauthoridMorris, HR=7401534652en_HK
dc.identifier.scopusauthoridHaslam, SM=7005784726en_HK
dc.identifier.scopusauthoridKhoo, KH=7102371762en_HK
dc.identifier.scopusauthoridClark, GF=7402874991en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK
dc.identifier.scopusauthoridDell, A=7103412653en_HK
dc.identifier.issnl0036-8075-

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