File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1074/jbc.273.20.12120
- Scopus: eid_2-s2.0-0032524286
- PMID: 9575157
- WOS: WOS:000073629800022
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: The role of negative superhelicity and length of homology in the formation of paranemic joints promoted by RecA protein
Title | The role of negative superhelicity and length of homology in the formation of paranemic joints promoted by RecA protein |
---|---|
Authors | |
Issue Date | 1998 |
Publisher | American Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/ |
Citation | Journal Of Biological Chemistry, 1998, v. 273 n. 20, p. 12120-12127 How to Cite? |
Abstract | Escherichia coli RecA protein pairs homologous DNA molecules to form paranemic joints when there is an absence of a free end in the region of homologous contact. Paranemic joints are a key intermediate in homologous recombination and are important in understanding the mechanism for a search of hemology. The efficiency of paranemic joint formation depended on the length of homology and the topological forms of the duplex DNA. The presence of negative superhelicity increased the pairing efficiency and reduced the minimal length of homology required for paranemic joint formation. Negative superhelicity stimulated joint formation by favoring the initial unwinding of duplex DNA that occurred during the homology search and was not essential in the maintenance of the paired structure. Regardless of length of homology, formation of paranemic joints using circular duplex DNA required the presence of more than six negative supercoils. Above six negative turns, an increasing degree of negative superhelicity resulted in a linear increase in the pairing efficiency. These results support a model of two distinct kinds of DNA unwinding occurring in paranemic joint formation: an initial unwinding caused by heterologous contacts during synapsis and a later one during pairing of the homologous molecules. |
Persistent Identifier | http://hdl.handle.net/10722/147434 |
ISSN | 2020 Impact Factor: 5.157 2023 SCImago Journal Rankings: 1.766 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wong, BC | en_US |
dc.contributor.author | Chiu, SK | en_US |
dc.contributor.author | Chow, SA | en_US |
dc.date.accessioned | 2012-05-29T06:03:42Z | - |
dc.date.available | 2012-05-29T06:03:42Z | - |
dc.date.issued | 1998 | en_US |
dc.identifier.citation | Journal Of Biological Chemistry, 1998, v. 273 n. 20, p. 12120-12127 | en_US |
dc.identifier.issn | 0021-9258 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/147434 | - |
dc.description.abstract | Escherichia coli RecA protein pairs homologous DNA molecules to form paranemic joints when there is an absence of a free end in the region of homologous contact. Paranemic joints are a key intermediate in homologous recombination and are important in understanding the mechanism for a search of hemology. The efficiency of paranemic joint formation depended on the length of homology and the topological forms of the duplex DNA. The presence of negative superhelicity increased the pairing efficiency and reduced the minimal length of homology required for paranemic joint formation. Negative superhelicity stimulated joint formation by favoring the initial unwinding of duplex DNA that occurred during the homology search and was not essential in the maintenance of the paired structure. Regardless of length of homology, formation of paranemic joints using circular duplex DNA required the presence of more than six negative supercoils. Above six negative turns, an increasing degree of negative superhelicity resulted in a linear increase in the pairing efficiency. These results support a model of two distinct kinds of DNA unwinding occurring in paranemic joint formation: an initial unwinding caused by heterologous contacts during synapsis and a later one during pairing of the homologous molecules. | en_US |
dc.language | eng | en_US |
dc.publisher | American Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/ | en_US |
dc.relation.ispartof | Journal of Biological Chemistry | en_US |
dc.subject.mesh | Bacteriophage Phi X 174 - Genetics | en_US |
dc.subject.mesh | Dna, Bacterial - Genetics - Metabolism | en_US |
dc.subject.mesh | Dna, Circular - Genetics - Metabolism | en_US |
dc.subject.mesh | Dna-Binding Proteins - Metabolism | en_US |
dc.subject.mesh | Escherichia Coli - Genetics | en_US |
dc.subject.mesh | Microvirus - Genetics | en_US |
dc.subject.mesh | Rec A Recombinases - Metabolism | en_US |
dc.subject.mesh | Recombination, Genetic | en_US |
dc.subject.mesh | Sequence Homology, Nucleic Acid | en_US |
dc.title | The role of negative superhelicity and length of homology in the formation of paranemic joints promoted by RecA protein | en_US |
dc.type | Article | en_US |
dc.identifier.email | Wong, BC:bcwwong@hkucc.hku.hk | en_US |
dc.identifier.authority | Wong, BC=rp00369 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1074/jbc.273.20.12120 | en_US |
dc.identifier.pmid | 9575157 | - |
dc.identifier.scopus | eid_2-s2.0-0032524286 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0032524286&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 273 | en_US |
dc.identifier.issue | 20 | en_US |
dc.identifier.spage | 12120 | en_US |
dc.identifier.epage | 12127 | en_US |
dc.identifier.isi | WOS:000073629800022 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Wong, BC=35733052400 | en_US |
dc.identifier.scopusauthorid | Chiu, SK=7202291671 | en_US |
dc.identifier.scopusauthorid | Chow, SA=7201827867 | en_US |
dc.identifier.issnl | 0021-9258 | - |