Culture of chondrocytes of hereditary multiple exostosis in vitro

Abstract

Aim: To observe growth and related biological characters by culturing chondrocytes of hereditary multiple exostosis in vitro from human and to establish bases for further research on the etiology of hereditary multiple exostosis. Methods: Chondrocytes were culture primarily from exostosis and passaged. The morphology of chondrocytes was observed by microscope. Meanwhile the abilities of cell proliferation and adhesive rates were detected and were compared with control cartilage chondrocytes. Results: The living chondrocytes still were 95% after complete digestion. After passaged, exostosis chondrocytes grew in mololayer culture had a stellate appearance with more sticklike structures in the cytoplasm and had many obvious granules. Proliferation of exostosis chondrocytes turned slowly with passaged incresed, and the obvious difference existed between the 2th passaged and the primary exostosis chondrocytes( P < 0.01, t = 3.203) Most of the primary exostosis chondrocytes could adhere dishes within 24 hours. But with passaged incresed, the adhesive abilities turned down. The obvious difference existed between the 4th passaged and the primary exostosis chondrocytes. (P < 0.01, t = 4.611). Conclusion: The morphology and biological characters (proliferation, adhesive ability) of exostosis chondrocytes are different from control normal cartilage chondrocytes. It can establish some culture bases for further etiology research of hereditary multiple exostosis.