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- Publisher Website: 10.1007/978-1-60761-738-9_6
- Scopus: eid_2-s2.0-84934439368
- PMID: 20694663
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Article: Raf/MEK/MAPK signaling stimulates the nuclear translocation and transactivating activity of FOXM1.
Title | Raf/MEK/MAPK signaling stimulates the nuclear translocation and transactivating activity of FOXM1. |
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Authors | |
Keywords | Cyclin B1 promoter FOXM1 G2/M hTERT-BJ1 Nuclear translocation Raf/MEK/MAPK signaling U0126 |
Issue Date | 2010 |
Citation | Methods In Molecular Biology (Clifton, N.J.), 2010, v. 647, p. 113-123 How to Cite? |
Abstract | The proliferation-associated transcription factor FOXM1 is essential for cell cycle progression into mitosis. Using synchronized human fibroblasts we detected, by immunostaining, that FOXM1 is localized predominantly in the cytoplasm in cells at late-G1 and S phases. Nuclear translocation occurs just before progression into the G2/M phase of the cell cycle and requires activity of the Raf/MEK/MAPK signaling pathway. Using pharmacological modulators, we demonstrated that activity of the Raf/MEK/MAPK pathway is both necessary and sufficient for the nuclear translocation of FOXM1. Consistent with FoxM1c being the major isoform expressed in proliferating fibroblasts, constitutively active MEK1 enhances the transactivating effect of FOXM1c, but not FOXM1b, on the cyclin B1 promoter in transient reporter assays. Here, we describe in detail the methods involved in generating these findings, which support the notion that FOXM1 is an effector of Raf/MEK/MAPK signaling in G2/M regulation. |
Persistent Identifier | http://hdl.handle.net/10722/147628 |
ISSN | 2023 SCImago Journal Rankings: 0.399 |
DC Field | Value | Language |
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dc.contributor.author | Ma, RY | en_US |
dc.contributor.author | Tong, TH | en_US |
dc.contributor.author | Leung, WY | en_US |
dc.contributor.author | Yao, KM | en_US |
dc.date.accessioned | 2012-05-29T06:05:05Z | - |
dc.date.available | 2012-05-29T06:05:05Z | - |
dc.date.issued | 2010 | en_US |
dc.identifier.citation | Methods In Molecular Biology (Clifton, N.J.), 2010, v. 647, p. 113-123 | en_US |
dc.identifier.issn | 1940-6029 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/147628 | - |
dc.description.abstract | The proliferation-associated transcription factor FOXM1 is essential for cell cycle progression into mitosis. Using synchronized human fibroblasts we detected, by immunostaining, that FOXM1 is localized predominantly in the cytoplasm in cells at late-G1 and S phases. Nuclear translocation occurs just before progression into the G2/M phase of the cell cycle and requires activity of the Raf/MEK/MAPK signaling pathway. Using pharmacological modulators, we demonstrated that activity of the Raf/MEK/MAPK pathway is both necessary and sufficient for the nuclear translocation of FOXM1. Consistent with FoxM1c being the major isoform expressed in proliferating fibroblasts, constitutively active MEK1 enhances the transactivating effect of FOXM1c, but not FOXM1b, on the cyclin B1 promoter in transient reporter assays. Here, we describe in detail the methods involved in generating these findings, which support the notion that FOXM1 is an effector of Raf/MEK/MAPK signaling in G2/M regulation. | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Methods in molecular biology (Clifton, N.J.) | en_US |
dc.subject | Cyclin B1 promoter | - |
dc.subject | FOXM1 | - |
dc.subject | G2/M | - |
dc.subject | hTERT-BJ1 | - |
dc.subject | Nuclear translocation | - |
dc.subject | Raf/MEK/MAPK signaling | - |
dc.subject | U0126 | - |
dc.subject.mesh | Active Transport, Cell Nucleus - Drug Effects | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Cell Line | en_US |
dc.subject.mesh | Cell Nucleus - Drug Effects - Metabolism | en_US |
dc.subject.mesh | Fibroblasts - Metabolism | en_US |
dc.subject.mesh | Forkhead Transcription Factors - Metabolism | en_US |
dc.subject.mesh | Genes, Reporter - Genetics | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Map Kinase Signaling System - Drug Effects | en_US |
dc.subject.mesh | Mice | en_US |
dc.subject.mesh | Mitogen-Activated Protein Kinase Kinases - Metabolism | en_US |
dc.subject.mesh | Transcriptional Activation - Drug Effects | en_US |
dc.subject.mesh | Raf Kinases - Metabolism | en_US |
dc.title | Raf/MEK/MAPK signaling stimulates the nuclear translocation and transactivating activity of FOXM1. | en_US |
dc.type | Article | en_US |
dc.identifier.email | Yao, KM:kmyao@hku.hk | en_US |
dc.identifier.authority | Yao, KM=rp00344 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1007/978-1-60761-738-9_6 | - |
dc.identifier.pmid | 20694663 | - |
dc.identifier.scopus | eid_2-s2.0-84934439368 | en_US |
dc.identifier.volume | 647 | en_US |
dc.identifier.spage | 113 | en_US |
dc.identifier.epage | 123 | en_US |
dc.identifier.scopusauthorid | Ma, RY=8323783700 | en_US |
dc.identifier.scopusauthorid | Tong, TH=8323783200 | en_US |
dc.identifier.scopusauthorid | Leung, WY=7201504543 | en_US |
dc.identifier.scopusauthorid | Yao, KM=7403234578 | en_US |
dc.identifier.issnl | 1064-3745 | - |