File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1111/j.1432-1033.1983.tb07745.x
- Scopus: eid_2-s2.0-0021062267
- PMID: 6688781
- WOS: WOS:A1983RQ53300014
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Evidence that 2-allyl-2-isopropylacetamide, phenobarbital and 3,5-diethoxycarbonyl-1,4-dihydrocollidine induce the same cytochrome P450 mRNA in chick embryo liver
Title | Evidence that 2-allyl-2-isopropylacetamide, phenobarbital and 3,5-diethoxycarbonyl-1,4-dihydrocollidine induce the same cytochrome P450 mRNA in chick embryo liver |
---|---|
Authors | |
Issue Date | 1983 |
Publisher | Blackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/EJB |
Citation | European Journal Of Biochemistry, 1983, v. 136 n. 2, p. 327-332 How to Cite? |
Abstract | The induction of cytochrome P450 in chick embryo liver has been studied using three different porphyrinogenic drugs, 2-allyl-2-isopropylacetamide, 3,5-diethoxycarbonyl-1,4-dihydrocollidine and phenobarbital. Pulse-labelling studies have shown that for each drug the cytochrome P450 synthesised either in ovo or in a wheat germ translation system reacted immunologically with antibody raised against the purified 2-allyl-2-isopropylacetamide-induced enzyme (M(r) = 50,000). To investigate whether this is due to the three drugs inducing the same protein or different proteins with common immunological determinants, nucleic acid hybridization studies have been carried out using a recently characterised 2-allyl-2-isopropylacetamide-induced cytochrome P450 cloned cDNA probe. It has been shown that the mRNA induced by each drug hybridizes within this probe and all are of similar size. The melting profile of the mRNA · cDNA hybrids indicates that the mRNAs induced by the three drugs have at least 98% homology with the cDNA probe. Restriction endonuclease digestions of total chick embryo genomal DNA and a chick cytochrome P450 genomal clone indicates that the cytochrome P450 gene homologous with the cDNA probe is represented in the genome only once. These results strongly suggest that the three drugs cause increased levels of the same cytochrome P450 mRNA, possibly due to enhanced expression of the same gene. Results are also presented which show that other cytochrome P450-inducing drugs, 3-methylcholanthrene, β-naphthoflavone or pregnenolone-16α-carbonitrile do not increase the level of the 2-allyl-2-isopropylacetamide-inducible mRNA but rather reduce it to a level which was lower than that of the untreated controls. |
Persistent Identifier | http://hdl.handle.net/10722/147739 |
ISSN | |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Brooker, JD | en_US |
dc.contributor.author | Srivastava, G | en_US |
dc.contributor.author | Borthwick, IA | en_US |
dc.date.accessioned | 2012-05-29T06:09:00Z | - |
dc.date.available | 2012-05-29T06:09:00Z | - |
dc.date.issued | 1983 | en_US |
dc.identifier.citation | European Journal Of Biochemistry, 1983, v. 136 n. 2, p. 327-332 | en_US |
dc.identifier.issn | 0014-2956 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/147739 | - |
dc.description.abstract | The induction of cytochrome P450 in chick embryo liver has been studied using three different porphyrinogenic drugs, 2-allyl-2-isopropylacetamide, 3,5-diethoxycarbonyl-1,4-dihydrocollidine and phenobarbital. Pulse-labelling studies have shown that for each drug the cytochrome P450 synthesised either in ovo or in a wheat germ translation system reacted immunologically with antibody raised against the purified 2-allyl-2-isopropylacetamide-induced enzyme (M(r) = 50,000). To investigate whether this is due to the three drugs inducing the same protein or different proteins with common immunological determinants, nucleic acid hybridization studies have been carried out using a recently characterised 2-allyl-2-isopropylacetamide-induced cytochrome P450 cloned cDNA probe. It has been shown that the mRNA induced by each drug hybridizes within this probe and all are of similar size. The melting profile of the mRNA · cDNA hybrids indicates that the mRNAs induced by the three drugs have at least 98% homology with the cDNA probe. Restriction endonuclease digestions of total chick embryo genomal DNA and a chick cytochrome P450 genomal clone indicates that the cytochrome P450 gene homologous with the cDNA probe is represented in the genome only once. These results strongly suggest that the three drugs cause increased levels of the same cytochrome P450 mRNA, possibly due to enhanced expression of the same gene. Results are also presented which show that other cytochrome P450-inducing drugs, 3-methylcholanthrene, β-naphthoflavone or pregnenolone-16α-carbonitrile do not increase the level of the 2-allyl-2-isopropylacetamide-inducible mRNA but rather reduce it to a level which was lower than that of the untreated controls. | en_US |
dc.language | eng | en_US |
dc.publisher | Blackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/EJB | en_US |
dc.relation.ispartof | European Journal of Biochemistry | en_US |
dc.subject.mesh | Acetamides - Pharmacology | en_US |
dc.subject.mesh | Allylisopropylacetamide - Pharmacology | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Chemical Phenomena | en_US |
dc.subject.mesh | Chemistry | en_US |
dc.subject.mesh | Chick Embryo | en_US |
dc.subject.mesh | Cytochrome P-450 Enzyme System - Biosynthesis | en_US |
dc.subject.mesh | Dna | en_US |
dc.subject.mesh | Dicarbethoxydihydrocollidine - Pharmacology | en_US |
dc.subject.mesh | Enzyme Induction - Drug Effects | en_US |
dc.subject.mesh | Liver - Enzymology | en_US |
dc.subject.mesh | Nucleic Acid Hybridization | en_US |
dc.subject.mesh | Phenobarbital - Pharmacology | en_US |
dc.subject.mesh | Pyridines - Pharmacology | en_US |
dc.subject.mesh | Rna, Messenger - Metabolism | en_US |
dc.title | Evidence that 2-allyl-2-isopropylacetamide, phenobarbital and 3,5-diethoxycarbonyl-1,4-dihydrocollidine induce the same cytochrome P450 mRNA in chick embryo liver | en_US |
dc.type | Article | en_US |
dc.identifier.email | Srivastava, G:gopesh@pathology.hku.hk | en_US |
dc.identifier.authority | Srivastava, G=rp00365 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1111/j.1432-1033.1983.tb07745.x | - |
dc.identifier.pmid | 6688781 | - |
dc.identifier.scopus | eid_2-s2.0-0021062267 | en_US |
dc.identifier.volume | 136 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.spage | 327 | en_US |
dc.identifier.epage | 332 | en_US |
dc.identifier.isi | WOS:A1983RQ53300014 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.issnl | 0014-2956 | - |