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- Publisher Website: 10.1002/jmv.1890450114
- Scopus: eid_2-s2.0-0028899254
- PMID: 7714494
- WOS: WOS:A1995QJ60600013
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Article: Evidence for lytic infection by Epstein-Barr virus in mucosal lymphocytes instead of nasopharyngeal epithelial cells in normal individuals
Title | Evidence for lytic infection by Epstein-Barr virus in mucosal lymphocytes instead of nasopharyngeal epithelial cells in normal individuals |
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Authors | |
Keywords | Immunohistochemistry In situ hybridization Latency Nasopharynx |
Issue Date | 1995 |
Publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/32763 |
Citation | Journal Of Medical Virology, 1995, v. 45 n. 1, p. 71-77 How to Cite? |
Abstract | Normal nasopharyngeal tissues from 23 individuals who died of causes unrelated to the upper respiratory system and had no evidence of Epstein-Barr virus (EBV)-related diseases were studied using in situ hybridisation (ISH) and immunohistochemistry for the detection of EBV RNA and expression of EBV proteins, respectively. ISH using 35S-labelled riboprobe for EBV EBER RNA showed occasional to a few EBER+ lymphocytes in the stroma of nasopharyngeal mucosa in 14/16 cases with available paraffin-embedded tissues. In addition, very rare intraepithelial EBER+ lymphocytes were also detected in 3/16 cases. However, in none of these cases was EBER detected in the epithelial cells. Similar results were obtained using a nonradioactive ISH method for EBER (Dako). In 3/23 cases, immunostaining using monoclonal antibodies for EBV proteins on cryostat sections showed occasional cells in the stroma expressing EBV nuclear antigen 2 (EBNA2), latent membrane protein-1 (LMP), and switch protein encoded by BZLF1 gene (ZEBRA) in two cases and only very rare LMP+ and ZEBRA+ cells in one other case. Double immunostaining combining alkaline phosphatase anti-alkaline phosphatase (APAAP) for CD markers and indirect immunofluorescence for LMP showed that the LMP+ cells were either CD19+ or less frequently CD3+, but none were CD68+. These results show that both B and T lymphocytes harbouring EBV can be found in the normal nasopharyngeal tissues. Interestingly, EBV proteins associated with lytic viral replication - diffuse early antigen (EA-D), viral capsid antigen (VCA), or membrane antigen (MA) - were also detected in rare cells in the stroma in one case, and in another case only one MA+ cell was detected. These results provide evidence for a lytic type of infection by EBV in the normal nasopharynx involving a very small proportion of stromal lymphocytes and support the view that nasopharyngeal lymphocytes can act as a reservoir for EBV in normal individuals. |
Persistent Identifier | http://hdl.handle.net/10722/148040 |
ISSN | 2023 Impact Factor: 6.8 2023 SCImago Journal Rankings: 1.560 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Tao, Q | en_HK |
dc.contributor.author | Srivastava, G | en_HK |
dc.contributor.author | Chan, ACL | en_HK |
dc.contributor.author | Chung, LP | en_HK |
dc.contributor.author | Loke, SL | en_HK |
dc.contributor.author | Ho, FCS | en_HK |
dc.date.accessioned | 2012-05-29T06:10:32Z | - |
dc.date.available | 2012-05-29T06:10:32Z | - |
dc.date.issued | 1995 | en_HK |
dc.identifier.citation | Journal Of Medical Virology, 1995, v. 45 n. 1, p. 71-77 | en_HK |
dc.identifier.issn | 0146-6615 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/148040 | - |
dc.description.abstract | Normal nasopharyngeal tissues from 23 individuals who died of causes unrelated to the upper respiratory system and had no evidence of Epstein-Barr virus (EBV)-related diseases were studied using in situ hybridisation (ISH) and immunohistochemistry for the detection of EBV RNA and expression of EBV proteins, respectively. ISH using 35S-labelled riboprobe for EBV EBER RNA showed occasional to a few EBER+ lymphocytes in the stroma of nasopharyngeal mucosa in 14/16 cases with available paraffin-embedded tissues. In addition, very rare intraepithelial EBER+ lymphocytes were also detected in 3/16 cases. However, in none of these cases was EBER detected in the epithelial cells. Similar results were obtained using a nonradioactive ISH method for EBER (Dako). In 3/23 cases, immunostaining using monoclonal antibodies for EBV proteins on cryostat sections showed occasional cells in the stroma expressing EBV nuclear antigen 2 (EBNA2), latent membrane protein-1 (LMP), and switch protein encoded by BZLF1 gene (ZEBRA) in two cases and only very rare LMP+ and ZEBRA+ cells in one other case. Double immunostaining combining alkaline phosphatase anti-alkaline phosphatase (APAAP) for CD markers and indirect immunofluorescence for LMP showed that the LMP+ cells were either CD19+ or less frequently CD3+, but none were CD68+. These results show that both B and T lymphocytes harbouring EBV can be found in the normal nasopharyngeal tissues. Interestingly, EBV proteins associated with lytic viral replication - diffuse early antigen (EA-D), viral capsid antigen (VCA), or membrane antigen (MA) - were also detected in rare cells in the stroma in one case, and in another case only one MA+ cell was detected. These results provide evidence for a lytic type of infection by EBV in the normal nasopharynx involving a very small proportion of stromal lymphocytes and support the view that nasopharyngeal lymphocytes can act as a reservoir for EBV in normal individuals. | en_HK |
dc.language | eng | en_US |
dc.publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/32763 | en_HK |
dc.relation.ispartof | Journal of Medical Virology | en_HK |
dc.subject | Immunohistochemistry | en_HK |
dc.subject | In situ hybridization | en_HK |
dc.subject | Latency | en_HK |
dc.subject | Nasopharynx | en_HK |
dc.subject.mesh | Adult | en_US |
dc.subject.mesh | Aged | en_US |
dc.subject.mesh | Aged, 80 And Over | en_US |
dc.subject.mesh | Epithelial Cells | en_US |
dc.subject.mesh | Epithelium - Virology | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Herpesviridae Infections - Virology | en_US |
dc.subject.mesh | Herpesvirus 4, Human - Isolation & Purification - Physiology | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Lymphocytes - Virology | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Middle Aged | en_US |
dc.subject.mesh | Nasal Mucosa - Virology | en_US |
dc.subject.mesh | Nasopharynx - Virology | en_US |
dc.subject.mesh | Rna, Viral - Analysis | en_US |
dc.subject.mesh | Viral Proteins - Analysis | en_US |
dc.subject.mesh | Virus Latency | en_US |
dc.subject.mesh | Virus Replication | en_US |
dc.title | Evidence for lytic infection by Epstein-Barr virus in mucosal lymphocytes instead of nasopharyngeal epithelial cells in normal individuals | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Srivastava, G: sgopesh@hkucc.hku.hk | en_HK |
dc.identifier.email | Chung, LP: lpchung@hkucc.hku.hk | en_HK |
dc.identifier.authority | Srivastava, G=rp00365 | en_HK |
dc.identifier.authority | Chung, LP=rp00249 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1002/jmv.1890450114 | en_HK |
dc.identifier.pmid | 7714494 | - |
dc.identifier.scopus | eid_2-s2.0-0028899254 | en_HK |
dc.identifier.hkuros | 5248 | - |
dc.identifier.volume | 45 | en_HK |
dc.identifier.issue | 1 | en_HK |
dc.identifier.spage | 71 | en_HK |
dc.identifier.epage | 77 | en_HK |
dc.identifier.isi | WOS:A1995QJ60600013 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Tao, Q=7102578359 | en_HK |
dc.identifier.scopusauthorid | Srivastava, G=7202242238 | en_HK |
dc.identifier.scopusauthorid | Chan, ACL=16047349300 | en_HK |
dc.identifier.scopusauthorid | Chung, LP=24315879100 | en_HK |
dc.identifier.scopusauthorid | Loke, SL=7006559512 | en_HK |
dc.identifier.scopusauthorid | Ho, FCS=7103408147 | en_HK |
dc.identifier.issnl | 0146-6615 | - |