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Article: Developmental regulation of apoptosis and BCL-2/BAX expression during B lymphopoiesis in normal and RAG-2-/- mouse bone marrow
Title | Developmental regulation of apoptosis and BCL-2/BAX expression during B lymphopoiesis in normal and RAG-2-/- mouse bone marrow |
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Authors | |
Issue Date | 1998 |
Publisher | Federation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/ |
Citation | Faseb Journal, 1998, v. 12 n. 5, p. A1080 How to Cite? |
Abstract | B cell genesis in mouse bone marrow (BM) represents a finely controlled balance between cell production and apoptotic cell selection. However, the molecular signals that modulate B cell selection in BM are unclear. In the present study, we have examined Bcl-2 and Bax protein expression among precursor B cells at phenotypically defined differentiation stages in normal mouse BM cell suspensions using Western blotting and flow cytometry. The mean concentration of Bcl-2 and Bax per cell changed during successive stages of B cell development, high Bax/Bcl-2 ratios generally correlating with high incidences of hypodiploid apoptotic cells detected by flow cytometry among large pre-B cells and immature B lymphocytes, respectively. Further, the B220+μ- early precursor B cells in RAG-2-/- mouse BM showed a high apoptotic incidence ex vivo and an accelerated rate of entry into the apoptotic pathway in short term culture, together with an elevated Bax/Bcl-2 protein ratio. Changes in Bax/Bcl-2 ratio, similarly correlated with modified apoptotic rates among precursor B cells, were also observed in interleukin-7 gene modified mice. These findings suggest that Bcl-2 family proteins are implicated in regulating apoptotic selection of precursor B cells during their development in mouse BM. |
Persistent Identifier | http://hdl.handle.net/10722/148481 |
ISSN | 2023 Impact Factor: 4.4 2023 SCImago Journal Rankings: 1.412 |
DC Field | Value | Language |
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dc.contributor.author | Osmond, DG | en_US |
dc.contributor.author | Lu, L | en_US |
dc.date.accessioned | 2012-05-29T06:13:12Z | - |
dc.date.available | 2012-05-29T06:13:12Z | - |
dc.date.issued | 1998 | en_US |
dc.identifier.citation | Faseb Journal, 1998, v. 12 n. 5, p. A1080 | en_US |
dc.identifier.issn | 0892-6638 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/148481 | - |
dc.description.abstract | B cell genesis in mouse bone marrow (BM) represents a finely controlled balance between cell production and apoptotic cell selection. However, the molecular signals that modulate B cell selection in BM are unclear. In the present study, we have examined Bcl-2 and Bax protein expression among precursor B cells at phenotypically defined differentiation stages in normal mouse BM cell suspensions using Western blotting and flow cytometry. The mean concentration of Bcl-2 and Bax per cell changed during successive stages of B cell development, high Bax/Bcl-2 ratios generally correlating with high incidences of hypodiploid apoptotic cells detected by flow cytometry among large pre-B cells and immature B lymphocytes, respectively. Further, the B220+μ- early precursor B cells in RAG-2-/- mouse BM showed a high apoptotic incidence ex vivo and an accelerated rate of entry into the apoptotic pathway in short term culture, together with an elevated Bax/Bcl-2 protein ratio. Changes in Bax/Bcl-2 ratio, similarly correlated with modified apoptotic rates among precursor B cells, were also observed in interleukin-7 gene modified mice. These findings suggest that Bcl-2 family proteins are implicated in regulating apoptotic selection of precursor B cells during their development in mouse BM. | en_US |
dc.language | eng | en_US |
dc.publisher | Federation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/ | en_US |
dc.relation.ispartof | FASEB Journal | en_US |
dc.title | Developmental regulation of apoptosis and BCL-2/BAX expression during B lymphopoiesis in normal and RAG-2-/- mouse bone marrow | en_US |
dc.type | Article | en_US |
dc.identifier.email | Lu, L:liweilu@hkucc.hku.hk | en_US |
dc.identifier.authority | Lu, L=rp00477 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.scopus | eid_2-s2.0-33749364038 | en_US |
dc.identifier.volume | 12 | en_US |
dc.identifier.issue | 5 | en_US |
dc.identifier.spage | A1080 | en_US |
dc.publisher.place | United States | en_US |
dc.identifier.issnl | 0892-6638 | - |