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- Publisher Website: 10.1049/iet-nbt.2010.0023
- Scopus: eid_2-s2.0-84861503069
- PMID: 22423865
- WOS: WOS:000301380000002
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Article: Hepatitis B surface antigen-antibody interactions studied by optical tweezers
| Title | Hepatitis B surface antigen-antibody interactions studied by optical tweezers | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Authors | |||||||||
| Issue Date | 2012 | ||||||||
| Publisher | The Institution of Engineering and Technology. | ||||||||
| Citation | IET Nanobiotechnology, 2012, v. 6 n. 1, p. 9-15 How to Cite? | ||||||||
| Abstract | The protein-protein interactions between hepatitis B surface antigen (HBsAg) and its antibodies (anti-HBs) were studied by measuring the binding force between microspheres coated with such proteins using optical tweezers. The interaction force between the protein-coated microspheres was found to be strongly influenced by the acidity of the surrounding liquid medium, as well as the experimental temperature, and it reaches a maximum value at around pH 7.5 and temperature around 37 degrees C. By measuring the protein distribution on the surfaces of the microspheres and their contact areas using scanning electron microscopy, the specific binding force between an HBsAg and anti-HBs protein pair is estimated to be around 4.8 pN at the optimum pH value and temperature at an applied loading rate of around 1 pN/s. © 2012 The Institution of Engineering and Technology. | ||||||||
| Persistent Identifier | http://hdl.handle.net/10722/149089 | ||||||||
| ISSN | 2023 Impact Factor: 3.8 2023 SCImago Journal Rankings: 0.512 | ||||||||
| ISI Accession Number ID |
Funding Information: The work described in this paper was supported by grants from the Research Grants Council (Project No. HKU 716908E), as well as from the University Grants Committee (Project No. SEG-HKU06) of the Hong Kong Special Administration Region, P.R. China. A. H. W. Ngan would like to acknowledge support in the form of a Senior Research Fellowship from the Croucher Foundation. | ||||||||
| References | |||||||||
| Grants |
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Zhou, ZL | en_HK |
| dc.contributor.author | Tang, B | en_HK |
| dc.contributor.author | Ngan, AHW | en_HK |
| dc.contributor.author | Dong, ZN | en_HK |
| dc.contributor.author | Wu, YS | en_HK |
| dc.date.accessioned | 2012-06-22T06:23:40Z | - |
| dc.date.available | 2012-06-22T06:23:40Z | - |
| dc.date.issued | 2012 | en_HK |
| dc.identifier.citation | IET Nanobiotechnology, 2012, v. 6 n. 1, p. 9-15 | en_HK |
| dc.identifier.issn | 1751-8741 | en_HK |
| dc.identifier.uri | http://hdl.handle.net/10722/149089 | - |
| dc.description.abstract | The protein-protein interactions between hepatitis B surface antigen (HBsAg) and its antibodies (anti-HBs) were studied by measuring the binding force between microspheres coated with such proteins using optical tweezers. The interaction force between the protein-coated microspheres was found to be strongly influenced by the acidity of the surrounding liquid medium, as well as the experimental temperature, and it reaches a maximum value at around pH 7.5 and temperature around 37 degrees C. By measuring the protein distribution on the surfaces of the microspheres and their contact areas using scanning electron microscopy, the specific binding force between an HBsAg and anti-HBs protein pair is estimated to be around 4.8 pN at the optimum pH value and temperature at an applied loading rate of around 1 pN/s. © 2012 The Institution of Engineering and Technology. | en_HK |
| dc.language | eng | en_US |
| dc.publisher | The Institution of Engineering and Technology. | en_HK |
| dc.relation.ispartof | IET Nanobiotechnology | en_HK |
| dc.subject.mesh | Antigen-antibody complex - chemistry | - |
| dc.subject.mesh | Hepatitis B antibodies - chemistry - immunology | - |
| dc.subject.mesh | Hepatitis B surface antigens - chemistry - immunology | - |
| dc.subject.mesh | Optical tweezers | - |
| dc.subject.mesh | Protein binding | - |
| dc.title | Hepatitis B surface antigen-antibody interactions studied by optical tweezers | en_HK |
| dc.type | Article | en_HK |
| dc.identifier.email | Tang, B: tangbin@hku.hk | en_HK |
| dc.identifier.email | Ngan, AHW: hwngan@hkucc.hku.hk | - |
| dc.identifier.authority | Tang, B=rp00081 | en_HK |
| dc.identifier.authority | Ngan, AHW=rp00225 | - |
| dc.description.nature | link_to_subscribed_fulltext | - |
| dc.identifier.doi | 10.1049/iet-nbt.2010.0023 | en_HK |
| dc.identifier.pmid | 22423865 | - |
| dc.identifier.scopus | eid_2-s2.0-84861503069 | en_HK |
| dc.identifier.hkuros | 199918 | en_US |
| dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-84861503069&selection=ref&src=s&origin=recordpage | en_HK |
| dc.identifier.volume | 6 | en_HK |
| dc.identifier.issue | 1 | en_HK |
| dc.identifier.spage | 9 | en_HK |
| dc.identifier.epage | 15 | en_HK |
| dc.identifier.isi | WOS:000301380000002 | - |
| dc.publisher.place | United Kingdom | en_HK |
| dc.relation.project | Nanomechanical characterization of bone using AFM nanoindentation | - |
| dc.identifier.scopusauthorid | Wu, YS=55229269400 | en_HK |
| dc.identifier.scopusauthorid | Dong, ZN=35975825300 | en_HK |
| dc.identifier.scopusauthorid | Ngan, AHW=55229573100 | en_HK |
| dc.identifier.scopusauthorid | Tang, B=24554184100 | en_HK |
| dc.identifier.scopusauthorid | Zhou, ZL=23017545300 | en_HK |
| dc.identifier.issnl | 1751-8741 | - |