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Conference Paper: SIRT1 enhances endothelium-dependent relaxation through an eNOS-independent mechanism
Title | SIRT1 enhances endothelium-dependent relaxation through an eNOS-independent mechanism |
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Authors | |
Keywords | Biology |
Issue Date | 2012 |
Publisher | Federation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/ |
Citation | The 2012 Annual Meeting of Experimental Biology (EB 2012), San Diego, CA., 21-25 April 2012. In The FASEB Journal, 2012, v. 26 meeting abstract, no. 671.1 How to Cite? |
Abstract | SIRT1 is a class III NAD+-dependent deacetylase which involved in a wide range of cellular processes and possesses vascular protective effects. SIRT1 maintains vascular homeostasis by reducing endothelial activation, preventing endothelial senescence, and promoting endothelium-dependent relaxations of vascular smooth muscle. To elucidate the molecular mechanisms underlying the SIRT1-mediated vascular protection, an endothelium-specific SIRT1 transgenic mouse model was engineered. The vascular responsiveness of the thoracic aorta was determined using a Mulvany-Halpern wire myograph for the recording of isometric force. The results showed that selective over-expression of SIRT1 in endothelial cells significantly enhanced acetylcholine-evoked aortic relaxation in mice of different ages [8, 12, 16 and 20 weeks] and prevented high fat diet-induced endothelial dysfunction. The enhanced endothelium-dependent relaxation in the aorta of these SIRT1 transgenic mice persisted in the presence of L-NAME, an inhibitor of NO synthesis. Moreover, the eNOS activity was not significantly different from that in preparations of wild type mice. These findings suggest that an eNOS/NO independent mechanism is involved in the vascular regulation by SIRT1. |
Description | Session - 671. Endothelial Mechanisms - Poster: no. 671.1 |
Persistent Identifier | http://hdl.handle.net/10722/149246 |
ISSN | 2023 Impact Factor: 4.4 2023 SCImago Journal Rankings: 1.412 |
DC Field | Value | Language |
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dc.contributor.author | Xu, C | en_US |
dc.contributor.author | Vanhoutte, PM | en_US |
dc.contributor.author | Wang, Y | en_US |
dc.date.accessioned | 2012-06-22T06:32:05Z | - |
dc.date.available | 2012-06-22T06:32:05Z | - |
dc.date.issued | 2012 | en_US |
dc.identifier.citation | The 2012 Annual Meeting of Experimental Biology (EB 2012), San Diego, CA., 21-25 April 2012. In The FASEB Journal, 2012, v. 26 meeting abstract, no. 671.1 | en_US |
dc.identifier.issn | 0892-6638 | - |
dc.identifier.uri | http://hdl.handle.net/10722/149246 | - |
dc.description | Session - 671. Endothelial Mechanisms - Poster: no. 671.1 | - |
dc.description.abstract | SIRT1 is a class III NAD+-dependent deacetylase which involved in a wide range of cellular processes and possesses vascular protective effects. SIRT1 maintains vascular homeostasis by reducing endothelial activation, preventing endothelial senescence, and promoting endothelium-dependent relaxations of vascular smooth muscle. To elucidate the molecular mechanisms underlying the SIRT1-mediated vascular protection, an endothelium-specific SIRT1 transgenic mouse model was engineered. The vascular responsiveness of the thoracic aorta was determined using a Mulvany-Halpern wire myograph for the recording of isometric force. The results showed that selective over-expression of SIRT1 in endothelial cells significantly enhanced acetylcholine-evoked aortic relaxation in mice of different ages [8, 12, 16 and 20 weeks] and prevented high fat diet-induced endothelial dysfunction. The enhanced endothelium-dependent relaxation in the aorta of these SIRT1 transgenic mice persisted in the presence of L-NAME, an inhibitor of NO synthesis. Moreover, the eNOS activity was not significantly different from that in preparations of wild type mice. These findings suggest that an eNOS/NO independent mechanism is involved in the vascular regulation by SIRT1. | - |
dc.language | eng | en_US |
dc.publisher | Federation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/ | - |
dc.relation.ispartof | The FASEB Journal | en_US |
dc.subject | Biology | - |
dc.title | SIRT1 enhances endothelium-dependent relaxation through an eNOS-independent mechanism | en_US |
dc.type | Conference_Paper | en_US |
dc.identifier.email | Vanhoutte, PM: vanhoutt@hku.hk | en_US |
dc.identifier.email | Wang, Y: yuwanghk@hku.hk | en_US |
dc.identifier.authority | Vanhoutte, PM=rp00238 | en_US |
dc.identifier.authority | Wang, Y=rp00239 | en_US |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.hkuros | 200067 | en_US |
dc.identifier.volume | 26 | - |
dc.identifier.issue | meeting abstract | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0892-6638 | - |